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European Journal of Wildlife Research

, Volume 60, Issue 3, pp 547–550 | Cite as

A DNA assay for rapid discrimination between beaver species as a tool for alien species management

  • R. McEwing
  • C. Frosch
  • F. Rosell
  • R. Campbell-PalmerEmail author
Short Communication

Abstract

The confirmed presence of alien North American beavers in some regions of Eurasia may compete with and hinder the successful recolonisation of the native Eurasian species back to its former range. Distinguishing the two species in the field can be problematic, time consuming and expensive, thereby potentially limiting appropriate conservation actions. Here, a rapid and inexpensive genetic SNP assay is described that can separate the two species from either non-invasively collected samples or samples taken directly from restrained individuals. We applied these new genetic assays to free-living beavers of unknown origin sampled in Scotland.

Keywords

Castor fiber Castor canadensis Real-time PCR SNP Wildlife management Non-invasive sampling 

Notes

Acknowledgments

We are grateful to Stefanie Venske (Nature Erlebnis Zentrum Wappenschmiiede, Germany) for providing samples as well as Casten Nowak, Robert Kraus (Conservation Genetics Group, Senckenberg, Germany), Helen Senn (WildGenes Laboratory, UK) for discussions, and continued support from Martin Gaywood, (Scottish Natural Heritage), Liz Haliwell, (Countryside Council for Wales) and the Scottish Beaver Trial. This work was funded by The Royal Zoological Society of Scotland.

References

  1. Danilov P, Kanshiev V, Fyodorov F (2011a) Differences of the morphology of the North American and Eurasian beavers in Karelia. In: Sjöberg G, Ball JP (eds) Restoring the European Beaver: 50 Years of Experience. Pensoft Publishers, Sofia, pp 49–54Google Scholar
  2. Danilov P, Kanshiev V, Fyodorov F (2011b) Characteristics of North American and Eurasian beaver ecology in Karelia. In: Sjöberg G, Ball JP (eds) Restoring the European Beaver: 50 Years of Experience. Pensoft Publishers, Sofia, pp 55–72Google Scholar
  3. Dewas M, Herr J, Schley L, Angst C, Manet B, Landry P, Catusee M (2012) Recovery and status of native and introduced beavers Castor fiber and Castor canadensis in France and neighbouring countries. Mammal Rev 42:144–165CrossRefGoogle Scholar
  4. Djoshkin W, Safonow W (1972) Die Biber der Alten und der Neuen Welt. Neue Brehm Bücherei 437, Wittenberg-LutherstadtGoogle Scholar
  5. Durka W, Babik W, Ducroz JK, Heidecke D, Rosell F, Samjaa R, Stubbe M (2005) Mitochondrial phylogeography of the Eurasian beaver Castor fiber L. Mol Ecol 14:3843–3856Google Scholar
  6. Halley DJ, Rosell F (2002) The beaver’s reconquest of Eurasia: status, population development and management of a conservation success. Mammal Rev 32:153–178CrossRefGoogle Scholar
  7. Herr J, Schley L (2009) Barbed wire hair traps as a tool for remotely collecting hair samples from beavers (Castor sp.). Lutra 52:123–127Google Scholar
  8. Horn S, Durka W, Wolf R, Ermala A, Stubbe A, Stubbe M, Hofreiter M (2011) Mitochondrial genomes reveal slow rates of molecular evolution and the timing of speciation in beavers (Castor), one of the largest rodent species. PLoS One 6:e14622. doi: 10.1371/journal.pone.0014622 PubMedCentralCrossRefPubMedGoogle Scholar
  9. Kuehn R, Schwab G, Schröder W, Rottmann O (2000) Differentiation of Castor fiber and Castor canadensis by non-invasive molecular methods. Zoo Biol 19:511–515CrossRefPubMedGoogle Scholar
  10. Lahti S, Helminen M (1974) The beaver Castor fiber (L.) and C. canadensis (Kuhl) in Finland. Acta Theriol 19:177–189CrossRefGoogle Scholar
  11. Lavrov VL (1996) Hybridization between Castor fiber orientoeuropaeus (Lavr, 1981) and Castor canadensis (Kühl, 1820). Bull Mosc Soc Nat Biol Ser 101:20–21 (In Russian with English summary)Google Scholar
  12. Lavrov LS, Orlov VN (1973) Karyotypes and taxonomy of modern beavers (Castor, Castoridae, Mammalia). Zoologicheskii Zhurnal 52:734–743 (In Russian with English summary)Google Scholar
  13. McEwing R, Kitchener AC, Holleley C, Kilshaw K, O’Donoghue P (2011) An allelic discrimination SNP assay for distinguishing the mitochondrial lineages of European wildcats and domestic cats. Conserv Genet Resour 4:163–165CrossRefGoogle Scholar
  14. Michaux J, Manet B, Herr J, Eugene M, Dalbeck L, Denne R, Schwoerer ML, Venske S, Rosell F, Schley L (2011) Current status of the distribution of the North American beaver (Castor canadensis) in the greater region of Belgium, Luxembourg and Western Germany. Abstract, 85th Annual Conference of the German Society of Mammalogy, 13–17 September 2011, LuxembourgGoogle Scholar
  15. Morin PA, Luikart G, Wayne RK, The SNP workshop group, Morin PA, Luikart G, Wayne RK (2004) SNP’s in ecology, evolution and conservation. Trends Ecol Evol 19:208–216CrossRefGoogle Scholar
  16. Osborn DJ (1955) Techniques of sexing beaver, Castor canadensis. J Mammal 36:141–142CrossRefGoogle Scholar
  17. Parker H, Nummi P, Hartman G, Rosell F (2012) Invasive North American beaver Castor canadensis in Eurasia: a review of potential consequences and strategy for eradication. Wildl Biol 18:354–365CrossRefGoogle Scholar
  18. Rosell F, Sun L (1999) Use of anal gland secretion to distinguish the two beaver species Castor canadensis and C. fiber. Wildl Biol 5:119–123Google Scholar
  19. Rosell F, Bozser O, Collen P, Parker H (2005) Ecological impact of beavers Castor fiber and Castor canadensis and their ability to modify ecosystems. Mammal Rev 32:248–276CrossRefGoogle Scholar
  20. Zurowski W (1983) Worldwide beaver symposium, Helsinki 1982: opening remarks. Acta Zool Fenn 174:85–86Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • R. McEwing
    • 1
  • C. Frosch
    • 2
  • F. Rosell
    • 3
  • R. Campbell-Palmer
    • 3
    • 4
    Email author
  1. 1.WildGenes LaboratoryRoyal Zoological Society of ScotlandEdinburghUK
  2. 2.Conservation Genetics GroupSenckenberg Research Institute and Natural History Museum FrankfurtGelnhausenGermany
  3. 3.Faculty of Arts and Sciences, Department of Environmental and Health StudiesTelemark University CollegeBø i TelemarkNorway
  4. 4.Conservation ProgrammesRoyal Zoological Society of ScotlandEdinburghUK

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