Abstract
Cross-species microsatellite amplification is an effective way of obtaining microsatellite loci for closely related taxa in bird species. The Reeves's pheasant, Syrmaticus reevesii, is a vulnerable species endemic to China. To improve population genetics and parentage analysis studies in this species, we obtained nine polymorphic microsatellite markers, in addition to the nine markers previously isolated, from the cross-species amplification of 52 markers. The number of alleles per locus varied between two and 12 with expected heterozygosity ranging from 0.298 to 0.714 (n = 107). The success rates of simulated paternity tests using CERVUS software improved at different confidence levels after adding these markers to the previous ones.
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Acknowledgements
The study was supported by the National Natural Science Foundation of China (no. 30570234) and the National Key Project of Scientific and Technical Supporting Programs funded by Ministry of Science and Technology of China (no. 2008BAC39B05). We thank Jian-qiang Li and Yang Qiu for the collection of samples, Dr. Shou-Hsien Li for the evaluation of microsatellite markers, and Lisa Gardner Barillas for the perfection of language.
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Wang, N., Chang, J., Gu, LY. et al. Polymorphic microsatellites in the Reeves's pheasant developed by cross-species amplification. Eur J Wildl Res 55, 627–629 (2009). https://doi.org/10.1007/s10344-009-0307-y
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DOI: https://doi.org/10.1007/s10344-009-0307-y