Abstract
Blood biochemical parameters may provide useful information about the physical condition of the individual, making them a useful tool in ecological studies. However, to avoid biases, factors affecting circulating levels of plasma metabolites must be investigated. In this paper, we analyse the effect of daytime sampling hour on seven plasma metabolites in adult red-legged partridges (Alectoris rufa) with free access to food during the breeding season. We found that sampling hour affected circulating levels of glucose and triglycerides but not those of cholesterol, total protein, uric acid, urea or albumin. A sex effect was found only for glucose, uric acid, urea and triglycerides. Repeated sampling affected all the parameters studied. These results suggest that the effect of sex, sampling hour and repeated sampling should be carefully controlled for (methodologically or by statistical procedures) to avoid undesirable sources of error at least in some blood parameters.
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References
Alonso-Alvarez C (2005) Age-dependent changes in plasma biochemistry of yellow-legged gulls (Larus cachinnans). Comp Biochem Physiol A 140:512–518
Alonso-Alvarez C, Velando A (2003) Female body condition and brood sex ratio in yellow-legged gulls Larus cachinnans. Ibis 145:220–226
Alonso-Alvarez C, Ferrer M, Velando A (2002) The plasmatic index of body condition in yellow-legged gulls Larus cachinnans: a food-controlled experiment. Ibis 144:147–149
Buchanan KL, Evans MR, Goldsmith AR, Bryant DM, Rowe L (2001) Testosterone influences basal metabolic rate in house sparrows: a new cost of dominance signalling? Proc R Soc Lond B 268:1337–1344
Buyse J, Janssens K, Van der Geyten S, Van As P, Decuypere E, Darras VM (2002) Pre- and postprandial changes in plasma hormone and metabolite levels and hepatic deiodinase activities in meal-fed broiler chickens. Br J Nutr 88:641–653
Campbell TW (1994) Hematology. In: Ritchie BW, Harrison GH, Harrison LR (eds) Avian medicine: principles and application. Wingers Publishing, Lake Worth, pp 176–198
Ferrer M (1993) Blood chemistry studies in birds: some applications to ecological problems. Trends Comp Biochem Physiol 1:1031–1044
Ferrer M, Amat JA, Viñuela J (1994) Daily variations of blood chemistry values in the chinstrap penguin (Pygoscelis antartica) during the Antarctic summer. Comp Biochem Physiol 107A:81–84
García-Rodríguez T, Ferrer M, Recio MF, Castroviejo J (1987) Circadian rhythms of determined blood chemistry values in buzzards and eagle owls. Comp Biochem Physiol 88A:663–669
Gwinner E (1975) Circadian and circannual rhythms in birds. In: Farner DS, King JR (eds) Avian biology, vol 5. Academic, New York, pp 221–285
Herichova I, Zeman M, Jurani M, Lamosova D (2004) Daily rhythms of melatonin and selected biochemical parameters in plasma of Japanese quail. Avian Poultry Biol Rev 15:205–210
Jenni L, Jenni-Eiermann S (1996) Metabolic responses to diurnal feeding patterns during the postbreeding, moulting and migratory periods in passerine birds. Funct Ecol 10:73–80
Kaneko J, Harvey J, Bruss M (1997) Clinical biochemistry of domestic animals, 5th edn. Academic, New York, p 932
Kawai T (1973) Clinical aspects of the plasma proteins. Igaku Shoin, Tokyo
Krasnodebska-Depta A, Koncicki A (2002) Effect of short-term heat stress on some blood biochemical indices in turkeys. Medycyna Weterynaryjna 58:223–226
Littell RC, Henry PR, Ammerman CB (1998) Statistical analysis of repeated measures data using SAS procedures. J Anim Sci 76:1216–1231
Minick MC, Duke GE, Fishman SW, Harvey RB (1996) Effects of intragastric nutrients upon simultaneously determined plasma insulin, glucagon, insulin/glucagon ratios and glucose in red-tailed hawks, Buteo jamaicensis Gmelin. Comp Biochem Physiol A 113:173–179
Pérez-Rodríguez L, Alonso-Alvarez C, Viñuela J (2007) Repeated sampling but not sampling hour affects plasma carotenoid levels. Physiol Biochem Zool 80:250–254
Remage-Healey L, Romero LM (2002) Corticosterone and insulin interact to regulate plasma glucose but not lipid concentrations in moulting starlings. Gen Comp Endocrinol 129:88–94
Rodríguez P, Tortosa F, Millán J, Gortazar C (2004) Plasma chemistry reference values from captive red-legged partridges (Alectoris rufa). Br Poult Sci 45:565–567
Rodríguez P, Tortosa F, Villafuerte R (2005) The effects of fasting and refeeding on biochemical parameters in the red-legged partridge (Alectoris rufa). Comp Biochem Physiol A 140:157–164
Rodríguez P, Tortosa FS, Gortazar C (2006) Daily variations of blood biochemical parameters in the red-legged partridge (Alectoris rufa). Eur J Wildl Res 52:277–281
Schalm OW, Jain NC, Carrol EJ (1975) Veterinary hematology, 3rd edn. Lea and Febiger, Philadelphia, p 807
Stevens L (1996) Avian biochemistry and molecular biology. Cambridge Univ. Press, Cambridge
Vidal D, Naranjo V, Mateo R, Gortazar C, de la Fuente J (2006) Analysis of serum biochemical parameters in relation to Mycobacterium bovis infection of European wild boars (Sus scrofa) in Spain. Eur J Wildl Res 52(4):301–304
Wingfield JC, Hunt K, Breuner C, Dunlap K, Fowler GS, Freed L, Lepson J (1997) Environmental stress, field endocrinology, and conservation biology. In: Clemmos JR, Buchholz R (eds) Behavioral approaches to conservation in the wild. Cambridge Univ. Press, Cambridge, pp 95–131
Zaias J, Fox WP, Cray C, Altam NH (2000) Hematologic, plasma protein, and biochemical profiles of brown pelicans (Pelecanus occidentalis). Am J Vet Res 61:771–774
Acknowledgements
We thank Elisa Pérez, Salvador Jesús Luna and Francisco Buenestado for assistance during sample collection and Daniel J. Morrish for reviewing the English. Financial support was provided by the Research Project PAI-02-006 of the Junta de Comunidades de Castilla-La Mancha. L.P.R. was supported by an FPU grant of the Spanish Ministerio de Educacion y Ciencia. C.A.-A. was supported by a Ramon y Cajal fellowship (Ministerio de Educacion y Ciencia, Spain). M.M.-H. was supported by a postgraduate grant (I3P) from CSIC and the Research Proyect CGL2004-02568/BOS of the Spanish Ministerio de Educacion y Ciencia. This study was performed according to Spanish laws.
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Pérez-Rodríguez, L., Alonso-Alvarez, C., Martínez-Haro, M. et al. Variation in plasma biochemical parameters in captive adult red-legged partridges (Alectoris rufa) during daylight hours. Eur J Wildl Res 54, 21–26 (2008). https://doi.org/10.1007/s10344-007-0102-6
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DOI: https://doi.org/10.1007/s10344-007-0102-6