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A serological study on brucellosis in wild boars in Germany

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Abstract

A recent outbreak of brucellosis in an outdoor pig herd, where wild boars were identified as the most probable source of infection, prompted us to conduct a serological study on wild boars from five federal states of Germany. A total of 885 sera were examined using a combination of screening and confirmatory testing, i.e. indirect enzyme-linked immunosorbent assay (ELISA) followed by complement fixation and slow agglutination tests. Seroprevalences of Brucella suis antibodies in the various regions were between 0 and 28.5% in the ELISA. After confirmatory testing, the amount of positive sera was lower and reached only up to 12.1%, dependent on the method. Although wild boars usually harbour B. suis biovar 2, which is less virulent for humans, a zoonotic risk for persons dealing with these animals and their carcasses cannot be ruled out.

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References

  • Cvetnic Z, Mitak M, Ocepek M, Lojkic M, Terzic S, Jemersic L, Humski A, Habrun B, Sostaric B, Brstilo M, Krt B, Garin-Bastuji B (2003) Wild boars (Sus scrofa) as reservoirs of Brucella suis biovar 2 in Croatia. Acta Vet Hung 51:465–473

    Article  PubMed  CAS  Google Scholar 

  • Cvetnic Z, Toncic J, Spicic S, Lojkic M, Terzic S, Jemersic L, Humski A, Curic S, Mitak M, Habrun B, Brstilo M, Ocepek M, Krt B (2004) Brucellosis in wild boar (Sus scrofa) in the Republic of Croatia. Vet Med (Czech) 49:115–122

    Google Scholar 

  • Cvetnic Z, Spicic S, Curic S, Jukic B, Lojkic M, Albert D, Thiebaud M, Garin-Bastuji B (2005) Isolation of Brucella suis biovar 3 from horses in Croatia. Vet Rec 156:584–585

    PubMed  CAS  Google Scholar 

  • Dedek J (1983) Zur Epizootiologie der Schweinebrucellose unter besonderer Berücksichtigung von Erregerreservoiren. Mh Vet Med 38:852–856

    Google Scholar 

  • Deutz A, Fuchs K, Schuller W, Nowotny N, Auer H, Aspock H, Stunzner D, Kerbl U, Klement C, Kofer J (2003) Seroepidemiological studies of zoonotic infections in hunters in southeastern Austria-prevalences, risk factors, and preventive methods. Berl Munch Tierarztl Wochenschr 116 306–311 (article in German)

    PubMed  Google Scholar 

  • EC (2000) Commission Regulation (EC) No 535/2002 of 21 March 2002 amending Annex C to Council Directive 64/432/EEC and amending decision 2000/330/EC. EC Official J L80:22–28

    Google Scholar 

  • EEC (1964) Council Directive 64/432/EEC of 26 June 1964 on animal health problems affecting intra-Community trade in bovine animals and swine. EC Official J 121:1977–2012

    Google Scholar 

  • Gall D, Nielsen K (2004) Serological diagnosis of bovine brucellosis: a review of test performance and cost comparison. Rev Sci Tech 23:989–1002

    PubMed  CAS  Google Scholar 

  • Godfroid J, Käsbohrer A (2002) Brucellosis in the European Union and Norway at the turn of the twenty-first century. Vet Microbiol 90:135–145

    Article  PubMed  Google Scholar 

  • Godfroid J, Michel P, Uytterhaegen L, De Smedt C, Rasseneur F, Boelaert F, Saegerman C, Patigny X (1994) Brucellose enzootique a Brucella suis biovare 2 chez le sanglier (Sus scrofa) en Belgique. Ann Med Vet 138:263–268

    Google Scholar 

  • Godfroid J, Cloeckaert A, Liautard J-P, Kohler S, Fretin D, Walravens K, Garin-Bastuji B, Letesson J-J (2005) From the discovery of the Malta fever’s agent to the discovery of a marine mammal reservoir, brucellosis has continuously been a re-emerging zoonosis. Vet Res 36:313–326

    Article  PubMed  Google Scholar 

  • Gurtler M, Alter T, Kasimir S, Linnebur M, Fehlhaber K (2005) Prevalence of Yersinia enterocolitica in fattening pigs. J Food Prot 68:850–854

    PubMed  Google Scholar 

  • Haerer G, Nicolet J, Bacciarini L, Gottstein B, Giacometti M (2001) Causes of death, zoonoses, and reproduction in the European brown hare in Switzerland. Schweiz Arch Tierheilkd 143:193–201 (article in German)

    PubMed  CAS  Google Scholar 

  • Hayashidani H, Kanzaki N, Kaneko Y, Okatani AT, Taniguchi T, Kaneko K-I, Ogawa M (2002) Occurrence of Yersiniosis and Listeriosis in wild boars in Japan. J Wildl Dis 38:202–205

    PubMed  Google Scholar 

  • Heinritzi K, Aigner K, Erber M, Kersjes C, von Wangenheim B (1999) Brucellose und Aujeszky-Krankheit in einem Wildschweingatter (Fallbeschreibung). Tierarztl Prax 27:41–46

    CAS  Google Scholar 

  • Hurvell B, Lindberg AA (1973) Serological cross-reactions between different Brucella species and Yersinia enterocolitica immunochemical studies on phenol-water extracted lipopolysaccharides from Brucella abortus and Yersinia enterocolitica IX. Acta Pathol Microbiol Scand [B] Microbiol Immunol 81:113–119

    CAS  Google Scholar 

  • Kautzsch S, Seyfarth D, Schone R, Stehmann R (1995) An outbreak of brucellosis in pigs and conclusions for characteristics of this epidemic disease. Berl Munch Tierarztl Wochenschr 108:201–205 (article in German)

    PubMed  CAS  Google Scholar 

  • OIE, World Organisation for Animal Health (2005) http://www.oie.int/hs2

  • Pannwitz S (1984) Serologische Übersichtsuntersuchungen auf Brucellose bei Wildschweinen. Mh Vet Med 39:627–628

    Google Scholar 

  • Paton NI, Tee N, Vu CH, Teo T (2001) Visceral abscesses due to Brucella suis infection in a retired pig farmer. Clin Infect Dis 32:129–130

    Article  Google Scholar 

  • Teyssou R, Morvan J, Leleu JP, Roumegou P, Goullin B, Carteron B (1989) A case of brucellosis in man due to Brucella suis biovar 2. Med Mal Infect 19:160–161 (article in French)

    Article  Google Scholar 

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Acknowledgements

The authors thank Michaela Ganss for excellent technical assistance, Günter Steinbach for his support and Volker Kaden for providing the samples from Mecklenburg-West Pomerania, Saxony-Anhalt and Baden-Wuerttemberg. We declare that the experiments comply with the current laws of Germany.

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Correspondence to F. Melzer.

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Melzer, F., Lohse, R., Nieper, H. et al. A serological study on brucellosis in wild boars in Germany. Eur J Wildl Res 53, 153–157 (2007). https://doi.org/10.1007/s10344-006-0072-0

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  • DOI: https://doi.org/10.1007/s10344-006-0072-0

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