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Mung Bean Genotypes Demonstrate a Correlative Response at Biochemical and Molecular Level Under Salinity Stress

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Abstract

Salt stress is one of the main environmental factors inhibiting the growth of plants because it adversely disturbs the balance between reactive oxygen species content and antioxidant defense, causing oxidative stress. In this research, the effect of salt stress at different concentrations of NaCl (3, 6, 9 dS m−1) was investigated on 5 mung bean genotypes (9, 1015, 1018, 1021 and Gohar), specifically for antioxidant activity and proline content. In this regard, the relative expression of key genes involved in proline synthesis (P5 CS, P5CR) and degradation (PDH) were also studied in the leaves of tolerant genotype (1015) and susceptible genotype (9) by qRT-PCR under only 9 dS m−1 NaCl. Given the results, the activity of antioxidant enzymes, including ascorbate peroxidase, catalase, and guaiacol peroxidase significantly were increased particularly in genotype 1015 under 9 dS m−1 NaCl. Salinity stress led the elevation of proline contents. As a result, proline accumulation increased due to P5 CS, P5CR upregulation, and PDH down-regulation. On the other hand, incrementing proline in tolerant genotype (1015) versus susceptible genotype (9) implicates the key role of proline in salinity stress withstanding. Conclusively, the study results could be efficiently applicable in advance plant breeding programs.

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Acknowledgements

We appreciate the University of Tehran for supporting the current research

Funding

This study was funded by University of Tehran with grant number: 7101007/6/05

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Authors and Affiliations

  1. Department of Agronomy and Plant breeding, College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran

    Nasim Khalifeh, Manijeh Sabokdast Nodehi, Alireza Abbasi & Sajjad Sobhanverdi

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  1. Nasim Khalifeh
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  2. Manijeh Sabokdast Nodehi
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  3. Alireza Abbasi
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  4. Sajjad Sobhanverdi
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Contributions

Corresponding author declares that all co-authors equally contributed to ending up this study until obtaining results and preparing manuscript. Contribution details follows here: Concept design; Manijeh Sabokdast Nodehi, and Alireza Abbasi., Material and methods; Nasim Khalifeh, Data analysis; Sajjad Sobhanverdi, and Nasim Khalifeh., Manuscript preparation; Manijeh Sabokdast Nodehi, and Sajjad Sobhanverdi.

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Correspondence to Manijeh Sabokdast Nodehi.

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Conflict of interest

N. Khalifeh, M. Sabokdast Nodehi, A. Abbasi and S. Sobhanverdi declare that they have no competing interests.

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Khalifeh, N., Sabokdast Nodehi, M., Abbasi, A. et al. Mung Bean Genotypes Demonstrate a Correlative Response at Biochemical and Molecular Level Under Salinity Stress. Gesunde Pflanzen 75, 911–919 (2023). https://doi.org/10.1007/s10343-022-00735-x

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