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Seed Priming Through Fungicides, Biocontrol Agents and Botanicals as Effective Method for Controlling Spot Blotch Pathogen, Bipolaris sorokiniana in Barley

Saatgutbehandlung mit Fungiziden, biologischen Bekämpfungsmitteln und Pflanzenextrakten als wirksame Methode zur Bekämpfung des Erregers der Blattfleckenkrankheit Bipolaris sorokiniana bei Gerste

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Abstract

This study was carried out to investigate the efficacy of fungicides (mancozeb/carbendazim), biocontrol agents (Trichoderma viride/Trichoderma harzianum) and botanicals (Eucalyptus globulus/Calotropis procera) as seed priming against barley spot-blotch pathogen, Bipolaris sorokiniana (Sacc.) Shoemaker under greenhouse conditions. Experimental results showed that B. sorokiniana inoculation caused significant reduction in growth, yield attributes and chlorophyll content over un-inoculated control. Disease related parameters which include the disease severity, lesion size and number of lesions were developed in pathogen inoculated plants. However, seed priming with fungicides, biocontrol agents, and botanical extracts, both in the presence or absence of pathogen, resulted in a substantial improvement in growth, yield attributes and chlorophyll content of barley plants over their respective control. Among treatments, seed priming with mancozeb at 300 mg/L had greater growth, yield attributes and chlorophyll content followed by other treatments in both inoculated and un-inoculated plants. Similarly, lowest growth, yield attributes and chlorophyll content were observed in pathogen inoculated plants primed with 20% C. procera leaf extracts. Seed priming followed by pathogen inoculation also reduced disease parameters significantly (p ≥ 0.05) over inoculated control. The principal component analysis (PCA) exhibited significant correlations between various studied attributes with 98.34% overall data variability.

Zusammenfassung

Diese Studie wurde durchgeführt, um die Wirksamkeit von Fungiziden (Mancozeb/Carbendazim), biologischen Bekämpfungsmitteln (Trichoderma viride/Trichoderma harzianum) und Pflanzenextrakten (Eucalyptus globulus/Calotropis procera) zur Saatgutbehandlung gegen den Blatfleckenkrankheitserreger Bipolaris sorokiniana (Sacc.) Shoemaker unter Gewächshausbedingungen bei Gerste zu untersuchen. Experimentelle Ergebnisse zeigten, dass die B. sorokiniana-Inokulation eine signifikante Verringerung des Wachstums, der Ertragseigenschaften und des Chlorophyllgehalts gegenüber der unbeimpften Kontrolle verursachte. Krankheitsbezogene Parameter, die die Schwere der Krankheit, die Größe der Läsion und die Anzahl der Läsionen einschließen, wurden in mit Pathogenen inokulierten Pflanzen entwickelt. Die Saatgutbehandlung mit Fungiziden, biologischen Bekämpfungsmitteln und Pflanzenextrakten führte jedoch sowohl in Gegenwart als auch in Abwesenheit des Krankheitserregers zu einer erheblichen Verbesserung des Wachstums, der Ertragsmerkmale und des Chlorophyllgehalts der Gerstenpflanzen im Vergleich zur jeweiligen Kontrolle. Unter den Behandlungen zeigte die Behandlung mit Mancozeb 300 mg/l ein stärkeres Wachstum, höhere Ertragsmerkmale und einen höheren Chlorophyllgehalt, gefolgt von den anderen Behandlungen sowohl bei beimpften als auch bei nicht beimpften Pflanzen. In ähnlicher Weise wurden das geringste Wachstum, die niedrigsten Ertragsmerkmale und der geringste Chlorophyllgehalt bei mit Krankheitserregern inokulierten Pflanzen beobachtet, die mit 20 %igen C. procera-Blattextrakten behandelt wurden. Die Behandlung des Saatguts, gefolgt von der Pathogen-Inokulation, reduzierte auch die Krankheitsparameter signifikant (p ≥ 0,05) gegenüber der inokulierten Kontrolle. Die Hauptkomponentenanalyse (PCA) zeigte signifikante Korrelationen zwischen verschiedenen untersuchten Attributen mit einer Gesamtvariabilität der Daten von 98,34 %.

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Acknowledgements

Authors are thankful to the Chairman, Department of Botany, Aligarh Muslim University, Aligarh, for providing facilities.

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  1. Plant Pathology and Nematology Laboratories, Department of Botany, Aligarh Muslim University, 202002, Aligarh, UP, India

    Lukman Ahamad

  2. Botany Section, Women’s College, Aligarh Muslim University, 202002, Aligarh, UP, India

    Razia K. Zaidi

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Correspondence to Lukman Ahamad.

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L. Ahamad and R.K. Zaidi declare that they have no competing interests.

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Ahamad, L., Zaidi, R.K. Seed Priming Through Fungicides, Biocontrol Agents and Botanicals as Effective Method for Controlling Spot Blotch Pathogen, Bipolaris sorokiniana in Barley. Gesunde Pflanzen 74, 501–510 (2022). https://doi.org/10.1007/s10343-022-00626-1

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