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The Biological Control of Fusarium oxysporum, the Causal Agent of Potato Rot

Die biologische Bekämpfung von Fusarium oxysporum, dem Erreger der Trockenfäule bei Kartoffeln

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Abstract

This study was carried out in vivo and in vitro to search the biological control possibilities of soil borne dry rot causal agent Fusarium oxysporum causing yield losses in potato. In this study, 2 Pantoea agglomerans (BRTB and RK-92), 2 Bacillus pumilus (RK-103 and TV-67C), 7 Bacillus subtilis (BAB-140, TV-12H, TV-6F, EK‑7, TV-17C, CP‑1 and TV-125A), 3 Bacillus megaterium (TV-103B, TV-87A and TV-91C), 1 Ochrobactrum anthropi (A-16B), 1 Agrobacterium radiobacter (A-16) and 1 Bacillus thuringiensis subsp. kurstaki (BAB-410) bacterial and 2 Trichoderma harzianum (ET 4 and ET 14) fungal isolates tested efficacy in previous studies were used. In pot trial, the experiment was established with the most effective five bacterial strains (BRTB 66.22%; RK-103, 50.90%; BAB-140 50.00%; TV-103B 49.10%; TV-12H 48.65% and TV-6F 48.20%) and two fungal isolates (ET 4; 69.44%, ET 14; 66.66%). BRTB, the most effective bacterial strain, prevented completely the development of the pathogen. Based on the application time of BRTB, infection was not observed on seedlings on growing from tubers inoculated with pathogen 4 h after dipping into the bacterial solution. In storage treatments, BRTB was the most efficacy isolate when compared with others. As a result, BRTB strain of Pantoea agglomerans can be candidate in the biological control of F. oxysporum.

Zusammenfassung

Diese Studie wurde in vivo und in vitro durchgeführt, um die Möglichkeiten der biologischen Bekämpfung des Erregers der Trockenfäule im Boden, Fusarium oxysporum, zu erforschen, der bei Kartoffeln Ertragsverluste verursacht. In dieser Studie wurden 2 Pantoea agglomerans-Isolate (BRTB und RK-92), 2 Bacillus pumilus-Isolate (RK-103 und TV-67C), 7 Bacillus subtilis-Isolate (BAB-140, TV-12H, TV-6F, EK‑7, TV-17C, CP‑1 und TV-125A), 3 Bacillus megaterium-Isolate (TV-103B, TV-87A und TV-91C), ein Ochrobactrum anthropi-Isolat (A-16B), ein Agrobacterium radiobacter-Isolat (A-16), ein Bacillus thuringiensis subsp. kurstaki-Isolat (BAB-410) und 2 Pilzisolate von Trichoderma harzianum (ET 4 und ET 14) verwendet, deren Wirksamkeit in früheren Studien getestet wurde. Im Topfversuch wurde der Versuch mit den fünf wirksamsten Bakterienstämmen (BRTB 66,22 %; RK-103, 50,90 %; BAB-140 50,00 %; TV-103B 49,10 %; TV-12H 48,65 % und TV-6F 48,20 %) und zwei Pilzisolaten (ET 4; 69,44 %, ET 14; 66,66 %) durchgeführt. BRTB, der wirksamste Bakterienstamm, verhinderte die Entwicklung des Pathogens vollständig. Basierend auf der Anwendungszeit von BRTB wurde 4 h nach dem Eintauchen in die bakterielle Lösung keine Infektion an Sämlingen beobachtet, die aus mit dem Erreger inokulierten Knollen gewachsen waren. Bei den Lagerungsbehandlungen war BRTB das wirksamste Isolat im Vergleich zu den anderen. Folglich kann der BRTB-Stamm von Pantoea agglomerans ein Kandidat für die biologische Bekämpfung von F. oxysporum sein.

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Acknowledgements

We would like to thank Prof. Dr. Recep KOTAN for providing the bacterial strains.

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  1. Department of Plant Protection, Faculty of Agriculture, Atatürk University, Erzurum, Turkey

    Gözdenur Çakar & Elif Tozlu

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Correspondence to Elif Tozlu.

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G. Çakar and E. Tozlu declare that they have no competing interests.

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This study is part of Gözdenur ÇAKAR’s master thesis accepted by Atatürk University Graduate School of Natural and Applied Sciences.

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Çakar, G., Tozlu, E. The Biological Control of Fusarium oxysporum, the Causal Agent of Potato Rot. Gesunde Pflanzen 74, 305–315 (2022). https://doi.org/10.1007/s10343-021-00610-1

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