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Nitric Oxide Treatment and Induced Genes Role Against Phytophthora infestans in Potato

Stickoxidbehandlung und die Rolle induzierter Gene gegen Phytophthora infestans bei Kartoffeln

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Abstract

Effects of sodium nitroprusside (SNP; nitric oxide donor) treatments on enhancement of secondary metabolites production, oxidative stress mediators (\(\mathrm{O}_{2}^{-}\)) accumulation and antioxidant defense enzymes of Potato Spunta Sp. suspension culture cells elicited by a fungal extract from phytophthora infestans mycelium. The obtained data confirmed the significant increase in various oxidative burst (super oxide anion, hydrogen peroxide and total glutathione) contents. The administration of various NO concentrations strongly decreased hydrogen peroxide concentration and superoxide anion levels. Moreover, the SNP treatments regulate elicitor-induced activation of phenylalanine ammonium-lyase and total soluble phenols accumulation. The highest concentrations of NO donor sodium nitroprusside potentiated elicitor-induced H2O2 production. On the other hand, the lowest H2O2 contents coincided with elicitation regulated various activities of enzymes superoxide-dismutase (SOD), ascorbate peroxidase (APX), catalase (CAT) and Phenyl alanine ammonia lyase (PAL) activity, the decrease in H2O2 concentration was probably due to a direct reduction interaction of NO-H2O2. On the other hand, the addition of these previous NO treatments affects mRNA peroxidase gene expression using RT-PCR techniques. In general, the addition of lower concentrations of nitric oxide reduce the mRNA peroxidase activity on contrary, the higher concentrations induced the mRNA peroxidase activity, which induce the hypersensitive reactions against fungus infection.

Zusammenfassung

Auswirkungen von Behandlungen mit Natrium-Nitroprussid (SNP, Stickoxiddonor) auf die Steigerung der Produktion von Sekundärmetaboliten, die Anreicherung von Mediatoren oxidativen Stresses (\(\mathrm{O}_{2}^{-}\)) und antioxidative Abwehrenzyme in Suspensionskulturzellen der Kartoffelsorte Spunta Sp., hervorgerufen durch ein Pilzextrakt aus Myzel der Art Phytophthora infestans. Die erzielten Daten bestätigten die signifikante Steigerung verschiedener oxidativer Bursts (Superoxidanion, Wasserstoffperoxid und Gesamtgehalt Glutathion). Die Verabreichung unterschiedlicher NO-Konzentrationen senkte die Wasserstoffperoxidkonzentration und die Superoxidanionwerte stark. Außerdem regulieren die SNP-Behandlungen die Elicitor-induzierte Aktivierung von Phenylalanin-Ammoniak-Lyase sowie die Anreicherung der Gesamtmenge löslicher Phenole. Die höchsten Konzentrationen NO-Donor-Natriumnitroprussid potenzierten die Elicitor-induzierte H2O2-Produktion. Andererseits fallen die geringsten H2O2-Werte mit verschiedenen Elicitor-regulierten Aktivitäten der Enzyme Superoxiddismutase (SOD), Ascorbatperoxidase (APX), Katalase (CAT) und Phenylalanin-Ammoniak-Lyase (PAL) zusammen. Der Rückgang in der H2O2-Konzentration lag wahrscheinlich an der direkten Reduktionsinteraktion mit NO-H2O2. Die Hinzufügung dieser o. g. NO-Behandlungen wirkt sich allerdings auch auf die mRNA-Peroxidase-Genexpression mit RT-PCR-Techniken aus. Allgemein senkt die Hinzufügung geringerer Konzentrationen Stickoxid die mRNA-Peroxidaseaktivität. Die höheren Konzentrationen induzierten aber die mRNA-Peroxidaseaktivität, was wiederum Überempfindlichkeitsreaktionen gegen die Pilzinfektion induziert.

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Authors and Affiliations

  1. Biochemistry Dept., Faculty of Agriculture, Cairo University, Gamma St, P. Box 12613, Giza, Cairo, Egypt

    Hossam S. El-Beltagi, Osama K. Ahmed & Gaber M. G. Shehab

  2. Medical Biochemistry Department, College of Medicine, Taif University, Taif, Saudi Arabia

    Gaber M. G. Shehab

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  1. Hossam S. El-Beltagi
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  2. Osama K. Ahmed
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  3. Gaber M. G. Shehab
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Correspondence to Hossam S. El-Beltagi.

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H.S. El-Beltagi, O.K. Ahmed and G.M.G. Shehab declare that they have no competing interests.

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El-Beltagi, H.S., Ahmed, O.K. & Shehab, G.M.G. Nitric Oxide Treatment and Induced Genes Role Against Phytophthora infestans in Potato. Gesunde Pflanzen 69, 171–183 (2017). https://doi.org/10.1007/s10343-017-0402-z

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