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Attenuation of Negative Effects of Saline Stress in two Lettuce Cultivars by Salicylic Acid and Glycine Betaine

Abschwächung der negativen Auswirkungen von Salzstress bei 2 Kopfsalatkultursorten durch Salicylsäure und Glycinbetain

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Abstract

Reducing destructive effects of lettuce plants irrigated with saline water was investigated in two field experiments conducted at Ismailia Governorate in 2012–2013 and 2013–2014. Two lettuce (Lactuca sativa L.) cultivars, Great Lakes and Balady, irrigated with saline water (3.22 dS/m) were treated using two levels of salicylic acid (SA) and glycine betaine (GB) as a foliar application (200, 400 ppm and 15, 30 Mm, respectively). Generally, application of SA and GB enhanced growth parameters, i. e. plant height; root length; number of leaves per plant; plant fresh and dry weight; leaf relative water content (LRWC); membrane permeability (MP); lipid peroxidation (MDA); chlorophyll a, b and carotenoid content in leaf; proline; total soluble sugars (TSS); superoxide dismutase (SOD) and peroxidase (POD) activity; and Na, K, Ca content. GB treatments significantly increased most recorded growth parameters, especially at 30 mM, while this treatment decreased values of MP, (MDA) and Na content in two lettuce cultivars.

Zusammenfassung

Die Minderung der destruktiven Wirkung der Bewässerung von Kopfsalatpflanzen mit salzhaltigem Wasser wurde in 2 Feldversuchen 2012–2013 und 2013–2014 im ägyptischen Gouvernement al-Isma’iliyya (Ismailia) untersucht. Die 2 Kopfsalatkultursorten (Lactuca sativa L.) Great Lakes und Balady wurden mit salzhaltigem Wasser (3,22 dS/m) gegossen und die Blätter mit 2 Dosierungen Salicylsäure (SA) und Glycinbetain (GB) behandelt (200 und 400 ppm bzw. 15 und 30 Mm). Allgemein steigerte die Anwendung von SA und GB die Wachstumsparameter, d. h. Pflanzenhöhe, Wurzellänge, Anzahl der Blätter pro Pflanze, Frisch- und Trockengewicht der Pflanze, Wassergehalt im Verhältnis zum gesamten Blatt („leaf relative water content“, LRWC), Membranpermeabilität (MP), Lipidperoxidation (MDA), Chlorophyll a und b, Karotinoidgehalt der Blätter, Prolin, Gesamtgehalt löslicher Zucker („total soluble sugars“, TSS), Aktivität von Superoxiddismutase (SOD) und Peroxidase (POD) sowie Gehalt an Na, K und Ca. GB-Behandlungen steigerten die meisten beobachteten Wachstumsparameter signifikant, insbesondere bei 30 mM, senkten aber die MP, die MDA und den Na-Gehalt in 2 Kopfsalatsorten.

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  1. Agricultural Botany Department, Faculty of Agriculture, Ain Shams University, Shoubra El-Kheima, Cairo, Egypt

    G. S. Khalifa, M. Abdelrassoul, Amira M. Hegazi & M. H. Elsherif

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  1. G. S. Khalifa
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  2. M. Abdelrassoul
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  3. Amira M. Hegazi
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Correspondence to Amira M. Hegazi.

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Khalifa, G.S., Abdelrassoul, M., Hegazi, A.M. et al. Attenuation of Negative Effects of Saline Stress in two Lettuce Cultivars by Salicylic Acid and Glycine Betaine. Gesunde Pflanzen 68, 177–189 (2016). https://doi.org/10.1007/s10343-016-0376-2

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