Zusammenfassung
Von 2003 bis 2007 wurden in verschiedenen Steinobstanbaugebieten in Südwestdeutschland visuelle Bonituren durchgeführt, um die Verbreitung der Europäischen Steinobstvergilbung (European stone fruit yellows, ESFY) in Deutschland zu untersuchen. Probennahmen in ausgewählten Referenzanlagen in den Regionen Neuwieder Becken, Rheinhessen, Vorderpfalz und Südpfalz erfolgten regelmäßig im Sommer und Frühjahr an Bäumen, die ESFY-typische Symptome aufwiesen, aber auch an Trieben von Bäumen mit unsicheren Symptomen. Alle Pflanzenproben wurden mit PCR (Polymerase Chain Reaction = Polymerase-Kettenreaktion) auf Infektion mit Candidatus Phytoplasma prunorum untersucht. Das Phytoplasma konnte in allen untersuchten Regionen auf den kultivierten Prunus-Arten P. armeniaca, P. persica und P. domestica nachgewiesen werden. Auf wilden Prunus-Arten wurden keine Infektionen gefunden. Die Krankheit war am stärksten auf Aprikose verbreitet, während Pfirsich und Pflaume weniger betroffen waren. Eine gute Korrelation zwischen visueller Bonitur und molekularem Nachweis des Erregers konnte für die typischen Sommer- und Wintersymptome sowohl bei P. armeniaca als auch P. persica ermittelt werden.
Mit regelmäßigen Fängen in den Referenzanlagen konnte die Populationsdynamik der Blattsaugerart Cacopsylla pruni über mehrere Jahre in Südwestdeutschland beschrieben werden. Die höchsten Populationsdichten wurden auf wilden Prunus-Arten gefunden. Alle gefangenen Einzeltiere wurden mittels PCR auf den Erreger getestet. Es konnte eine jährliche natürliche Infektionsrate von 1–2% aller Individuen von C. pruni ermittelt werden.
Abstract
From 2003 to 2007 surveys have been conducted in different stone fruit growing regions in southwest Germany to detect European stone fruit yellows (ESFY) disease in Germany. Samplings have been done regularly in selected reference orchards in the regions Neuwieder Becken, Rheinhessen, Vorderpfalz and Südpfalz in summer on trees showing ESFY typical symptoms as well as on branches of trees with unspecific symptoms. All samples have been analysed by PCR for infection with Candidatus Phytoplasma prunorum. The phytoplasma could be detected in all investigated regions on the cultivated Prunus species P. armeniaca, P. persica and P. domestica. No infection was found in wild Prunus species. The main spread of the disease appeared on apricot while peach and European plum were less affected. A good correlation between symptoms and molecular detection of the pathogen could be shown for the typical symptoms in summer and winter for apricot as well as for peach.
During regular psyllid captures in the reference orchards the population dynamics of Cacopsylla pruni could be described in southwest Germany for several years. By PCR-testing all collected insects individually a yearly natural infection rate of about 1–2% of all individuals of C. pruni could be calculated.
Literatur
Ahrens U, Seemüller E (1992) Detection of DNA of plant pathogenic mycoplasmalike organisms by a polymerase chain reaction that amplifies a sequence of the 16S rRNA gene. Phytopathology 82:828–832
Carraro L, Ferrini. F, Ermacora P, Loi N (2002) Role of wild Prunus species in the epidemiology of European stone fruit yellows. Plant Pathol 51:513–517
Carraro L, Ferrini F, Labonne G, Ermacora P, Loi N (2004) Seasonal infectivity of Cacopsylla pruni, vector of European stone fruit yellows phytoplasma. Ann Appl Biol 144:191–195
Carraro L, Loi N, Ermacora P (2001) Transmission characteristics of the European stone fruit yellows phytoplasma and its vector Cacopsylla pruni. Eur J Plant Pathol 107:695–700
Carraro L, Osler R, Loi N, Ermacora P, Refatti E (1998) Transmission of European stone fruit yellows phytoplasma by Cacopsylla pruni. J Plant Pathol 80:233–239
Chabrolin C (1924) Quelques maladies des arbres fruitiers de la Vallée du Rhône. Ann Epiphytes 10:265–333
Fialova R, Navratil M, Valova P, Kocourek F (2004) Epidemiology of Euroepan stone fruit yellows phytoplasma in Czech republic. Acta Hortic 657:483–487
Giunchedi L, Marani F, Credi R (1978) Mycoplasma-like bodies associated with plum decline (leptonecrosis). Phytopath Mediterranea 17:205–209
Jarausch W, Jarausch-Wehrheim B, Danet JL, Broquaire JM, Dosba F, Saillard C, Garnier M (2001) Detection and identification of European stone fruit yellows and other phytoplasmas in wild plants in the surroundings of apricot chlorotic leaf roll-affected orchards in southern France. Eur J Plant Pathol 107:209–217
Jarausch W, Lansac M, Saillard C, Broquaire JM, Dosba F (1998) PCR assay for specific detection of European stone fruit yellows phytoplasmas and its use for epidemiological studies in France. Eur J Plant Pathol 104:17–27
Jarausch B, Mühlenz I, Beck A, Lampe I, Harzer U, Jarausch W (2007) Epidemiology of European stone fruit yellows in Germany. Acta Hortic, in Druck
Kirkpatrick BC (1991) Mycoplasma-like organism-Plant and Invertebrate Pathogens. In: Balows A, Trüper HG, Dworkin M, Harder W, Schleifer KH (eds) The prokaryotes vol 2. Springer Verlag, New York, pp 4050–4067
Labonne G, Lichou J (2004) Data on the life cycle of Cacopsylla pruni, Psyllidae, vector of European stone fruit yellows (ESFY) phytoplasma in France. Acta Hortic 657:465–467
Laimer Da Camara Machado M, Paltrinieri S, Panzer V, Arthofer W, Strommer S, Martini M, Pondrelli M, Bertaccini A (2001) Presence of European stone fruit yellows (ESFY or 16SrX-B) phytoplasmas in apricots in Austria. Plant Pathol 50:130–135
Lederer W, Seemüller E (1992) Demonstration of mycoplasmas in Prunus species in Germany. J Phytopath 134:89–96
Lorenz K-H, Dosba F, Poggi Pollini C, Llacer G, Seemüller E (1994) Phytoplasma diseases of Prunus species in Europe are caused by genetically similar organism. Z Pflanzenkrankh Pflanzenschutz 101:567–575
Lorenz K-H, Schneider B, Ahrens U, Seemüller E (1995) Detection of the apple proliferation and pear decline phytoplasmas by PCR amplification of ribosomal and nonribosomal DNA. Phytopath 85:771–776
Maixner M, Ahrens U, Seemüller E (1995) Detection of the German grapevine yellows (Vergilbungskrankheit). MLO in grapevine, alternative hosts and a vector by a specific PCR procedure. Eur J Plant Pathol 101:241–250
Marcone C, Ragozzino A, Seemüller E (1996) European stone fruit yellows phytoplasma as the cause of peach vein enlargement and other yellows and decline diseases of stone fruits in southern Italy. J Phytopathol 144:559–564
Morvan G (1977) Apricot chlorotic leaf roll. EPPO Bulletin 7:37–55
Poggi Pollini C, Bissani R, Giunchedi L (2001) Occurrence of European stone fruit yellows phytoplasma (ESFYP) Infection in Peach Orchards in Northern-Central Italy. J Phytopath 149:725–730
Poggi Pollini C, Giunchedi L (1993) MLOs and suspected MLO diseases in fruit trees in Emiglia-Romana and Veneto. Phytopath Med 32:65–66
Ramel ME, Gugerli P (2004) Epidemiological Survey of European stone fruit yellows phytoplasma in Two Orchards in Western Switzerland. Acta Hortic 657:459–462
Schneider B, Seemüller E, Smart CD, Kirkpatrick BC (1995) Phylogenetic classification of plant pathogenic mycoplasma-like organisms or phytoplasmas. In: Razin S, TullyJG (eds.). Molecular and Diagnostic Procedures in Mycoplasmology, Vol. I. Academic Press, San Diego, pp 369–380
Seemüller E (1976) Investigations to demonstrate mycoplasmalike organisms in diseased plants by fluorescence microscopy. Acta Hortic 67:109–112
Seemüller E, Schneider B (2004) ‘Candidatus Phytoplasma mali’, ‘Candidatus Phytoplasma pyri’ and ‘Candidatus Phytoplasma prunorum’, the causal agents of apple proliferation, pear decline and European stone fruit yellows, respectively. Int J Syst Evol Microbiol 54:1217–1226
Danksagung
Wir bedanken uns für die Durchführung der Psyllidenfänge und Bonituren in Rheinhessen bei Werner Dahlbender, Günther Hensel und Wiebke Hein und für die Arbeiten im Neuwieder Becken bei Matthias Jönck.
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Jarausch, B., Mühlenz, I., Fuchs, A. et al. Untersuchungen zur Europäischen Steinobstvergilbung (ESFY) in Deutschland. Gesunde Pflanzen 59, 183–192 (2007). https://doi.org/10.1007/s10343-007-0169-8
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DOI: https://doi.org/10.1007/s10343-007-0169-8
Schlüsselwörter
- Candidatus Phytoplasma prunorum
- Prunus-Arten
- Symptomkorrelation
- Cacopsylla pruni
- Populationsdynamik
- Natürlicher Infektionsgrad