European Journal of Forest Research

, Volume 131, Issue 3, pp 669–681 | Cite as

In vitro morphogenic response and metal accumulation in Albizia lebbeck (L.) cultures grown under metal stress

  • Shahnaz Perveen
  • Mohammad AnisEmail author
  • I. M. Aref
Original Paper


An efficient regeneration protocol for rapid mass propagation and uptake of heavy metals in Albizia lebbeck (L.), a fast growing, medicinally as well as economically important timber yielding tree was developed. Nodal segments derived from a 20-year-old tree were cultured on MS (Murashige and Skoog) medium supplemented with 10 μM 6-Benzyladenine (BA) and 1 μM α-Naphthalene acetic acid (NAA) showed optimum shoot regeneration frequency (76.6%), number of shoots (23.2 ± 0.28) per explant and shoot length (2.86 ± 0.08 cm) after 10 weeks of culture. After standardizing a reliable protocol for micropropagation, effects of ZnSO4 (0.06–0.48 mM), CuSO4 (0.02–0.2 mM) and CdCl2 (0.0001–0.001 mM) on shoot morphogenesis were also assessed. The regenerated shoots maintained on maintenance medium (MS + 10.0 μM BA + 1.0 μM NAA) containing ZnSO4 (0.06 mM) showed maximum response in terms of shoot number (24.5 ± 0.83) and length (5.9 ± 0.05 cm) after 10 weeks of culture. Proline content showed an increasing trend while chlorophyll (a and b) content exhibited decreasing trend with an increased metal concentrations compared to MM cultures, and maximum increase in proline and decrease in chlorophyll content was recorded in cultures grown on Cd-enriched medium. Best rooting was accomplished on half strength MS medium with 2.0 μM IBA and ZnSO4 (0.06 mM). The plantlets thus obtained were successfully hardened and transferred to greenhouse with 75% survival rate and exhibited normal morphological characteristics compared to donor plant.


Proline Clonal propagation Metal tolerance AAS Chlorophyll content 





Indole-3-butyric acid


Double distilled water




Maintenance medium


Murashige and Skoog medium


α-Naphthalene acetic acid


Zinc sulfate




Copper sulfate


Cadmium chloride


Atomic Absorption Spectrophotometer



Financial support from the DST–FIST (2005–2010) and UGC- SAP (DRS-I) programmes (2009–2014), Govt of India, New Delhi, is highly appreciated. The authors are thankful to Prof. Athar Ali Khan, Department of Statistics, Aligarh Muslim University, Aligarh, for extending his cooperation in data analysis.


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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  1. 1.Plant Biotechnology Laboratory, Department of BotanyAligarh Muslim UniversityAligarhIndia
  2. 2.Department of Plant Production, College of Food and Agricultural SciencesKing Saud UniversityRiyadhSaudi Arabia

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