Advertisement

European Journal of Forest Research

, Volume 131, Issue 3, pp 669–681 | Cite as

In vitro morphogenic response and metal accumulation in Albizia lebbeck (L.) cultures grown under metal stress

  • Shahnaz Perveen
  • Mohammad AnisEmail author
  • I. M. Aref
Original Paper

Abstract

An efficient regeneration protocol for rapid mass propagation and uptake of heavy metals in Albizia lebbeck (L.), a fast growing, medicinally as well as economically important timber yielding tree was developed. Nodal segments derived from a 20-year-old tree were cultured on MS (Murashige and Skoog) medium supplemented with 10 μM 6-Benzyladenine (BA) and 1 μM α-Naphthalene acetic acid (NAA) showed optimum shoot regeneration frequency (76.6%), number of shoots (23.2 ± 0.28) per explant and shoot length (2.86 ± 0.08 cm) after 10 weeks of culture. After standardizing a reliable protocol for micropropagation, effects of ZnSO4 (0.06–0.48 mM), CuSO4 (0.02–0.2 mM) and CdCl2 (0.0001–0.001 mM) on shoot morphogenesis were also assessed. The regenerated shoots maintained on maintenance medium (MS + 10.0 μM BA + 1.0 μM NAA) containing ZnSO4 (0.06 mM) showed maximum response in terms of shoot number (24.5 ± 0.83) and length (5.9 ± 0.05 cm) after 10 weeks of culture. Proline content showed an increasing trend while chlorophyll (a and b) content exhibited decreasing trend with an increased metal concentrations compared to MM cultures, and maximum increase in proline and decrease in chlorophyll content was recorded in cultures grown on Cd-enriched medium. Best rooting was accomplished on half strength MS medium with 2.0 μM IBA and ZnSO4 (0.06 mM). The plantlets thus obtained were successfully hardened and transferred to greenhouse with 75% survival rate and exhibited normal morphological characteristics compared to donor plant.

Keywords

Proline Clonal propagation Metal tolerance AAS Chlorophyll content 

Abbreviations

BA

6-Benzyladenine

IBA

Indole-3-butyric acid

DDW

Double distilled water

Kn

Kinetin

MM

Maintenance medium

MS

Murashige and Skoog medium

NAA

α-Naphthalene acetic acid

ZnSO4

Zinc sulfate

chl

chlorophyll

CuSO4

Copper sulfate

CdCl2

Cadmium chloride

AAS

Atomic Absorption Spectrophotometer

Notes

Acknowledgments

Financial support from the DST–FIST (2005–2010) and UGC- SAP (DRS-I) programmes (2009–2014), Govt of India, New Delhi, is highly appreciated. The authors are thankful to Prof. Athar Ali Khan, Department of Statistics, Aligarh Muslim University, Aligarh, for extending his cooperation in data analysis.

References

  1. Agarwal V, Sharma K (2006) Phytotoxic effects of Cu, Zn, Cd and Pb on in vitro regeneration and concomitant protein changes in Holarrhena antidysentrica. Biol Plant 50:307–310CrossRefGoogle Scholar
  2. Agresti A (1996) An introduction to categorical data analysis. Wiley, New YorkGoogle Scholar
  3. Ahmad N, Anis M (2011) An efficient in vitro process for recurrent production of cloned plants of Vitex negundo L. Eur J Forest Res 130:135–144Google Scholar
  4. Ali G, Srivastava PS, Iqbal M (1998) Morphogenic response and proline content in Bacopa monniera cultures grown under copper stress. Plant Sci 138:191–195CrossRefGoogle Scholar
  5. Ali G, Srivastava PS, Iqbal M (2001) Responses of Bacopa monniera cultures to cadmium toxicity. Bull Environ Contam Toxicol 66:342–349PubMedCrossRefGoogle Scholar
  6. Almeida AAFD, Valle RR, Mielke MS, Gomes FP (2007) Tolerance and prospection of phytoremediator woody species of Cd, Pb, Cu and Cr. Braz J plant physiol 19:2Google Scholar
  7. Arnon DI (1949) Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiol 24:1–15PubMedCrossRefGoogle Scholar
  8. Arora M, Kiran B, Rani S, Rani A, Kaur B, Mittal N (2008) Heavy metal accumulation in vegetables irrigated with water from different sources. Food Chem 111:811–815CrossRefGoogle Scholar
  9. Ashraf M, Fooland MR (2007) Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environ Exp Bot 9:206–216CrossRefGoogle Scholar
  10. Balestrasse KB, Gallego SM, Benavides MP, Tomaro ML (2005) Polyamines and proline are affected by cadmium stress in nodules and roots of soybean plants. Plant Soil 270:343–353CrossRefGoogle Scholar
  11. Bates LS, Waldren RP, Teare JD (1973) Rapid determination of free proline for water stress studies. Plant Soil 39:205–207CrossRefGoogle Scholar
  12. Chen CT, Chen LM, Lin CC, Kao CH (2001) Regulation of proline accumulation in detached rice leaves exposed to excess copper. Plant Sci 160:283–290PubMedCrossRefGoogle Scholar
  13. Cobbett CS (2000) Phytochelatins and their roles in heavy metal detoxification. Plant Physiol 123:825–832PubMedCrossRefGoogle Scholar
  14. Dinakar N, Nagajyothi PC, Suresh S, Udaykiran Y, Damodharan T (2008) Phytotoxicity of cadmium on protein, proline and antioxidant enzyme activities in growing Arachis hypogeal L. seedlings. J Environ Sci 20:199–206CrossRefGoogle Scholar
  15. Gatti E (2008) Micropropagation of Ailanthus altissima and in vitro heavy metal tolerance. Biol Plant 52:146–148CrossRefGoogle Scholar
  16. George EF, Hall MA, Klerk GJD (2008) Plant propagation by tissue culture: the background, vol 1, 3rd edn. Springer, Dordrecht, pp 88–90Google Scholar
  17. Ghanaya AB, Charles G, Hourmant A, Hamida JB, Branchard M (2007) Morphological and physiological characteristics of rapeseed plants regenerated in vitro from thin cell layers in presence of zinc. CR Biologies 330:728–734CrossRefGoogle Scholar
  18. Ghanaya AB, Hourmant A, Cerantola S, Kervarec N, Cabon JY, Branchard M, Charles G (2010) Influence of zinc on soluble carbohydrate and free amino acid levels in rapeseed plants regenerated in vitro in the presence of zinc. Plant Cell Tiss Organ Cult 102:191–197CrossRefGoogle Scholar
  19. Guadagnini M, Herzig R, Erismann KH, Müller-Schärer H (1999) In vitro Züchtung, Selektion und Erprobung von metalakkumulierenden Tabakvarianten zur Bodensanierung. TerraTech 6:52–54Google Scholar
  20. Hall JL (2002) Cellular mechanism for heavy metal detoxification and tolerance. J Exp Bot 53:1–11PubMedCrossRefGoogle Scholar
  21. Htwe NN, Maziah M, Ling HC, Zaman FQ, Zain AM (2011) Response of some selected Malaysian rice genotype to callus induction under in vitro salt stress. Afri J Biotech 10:350–362Google Scholar
  22. Hussain TM, Chandrasekhar T, Gopal GR (2008) Micropropagation of Sterculia urens Roxb, an endangered tree species from intact seedlings. Afri J Biotechnol 7:095–101Google Scholar
  23. Jain N, Babbar SB (2000) Recurrent production of plants of black plum Syzygium cumini (L.) Skeels, a myrtaceous fruit tree, from in vitro cultured seedling explants. Plant Cell Rep 19:519–525CrossRefGoogle Scholar
  24. John R, Ahmad P, Gadgil K, Sharma S (2008) Effect of Cadmium and lead on growth biochemical parameters and uptake in Lamna polyrrhiza L. Plant Soil Environ 54:262–270Google Scholar
  25. Kaveriappa KM, Phillips LM, Trigiano RN (1997) Micropropagation of flowering dogwood (Cornus florida) from seedlings. Plant Cell Rep 16:485–489Google Scholar
  26. Khan MA, Ahmad I (2005) Multiple shoot induction and plant regeneration in Litchi (Litchi chinensis Sonn.). Int J Agri Biol 7:524–526Google Scholar
  27. Kiran B, Kudesia R, Rani M, Pal A (2009) Reclaiming degraded land in India through the cultivation of medicinal plants. Bot Res Int 2:174–181Google Scholar
  28. Kirtikar KR, Basu BD (1980) In: Singh B, Singh MP (eds) Indian medicinal plants, India, Part ii, p 937Google Scholar
  29. Kothari SL, Agarwal K, Kumar S (2004) Inorganic nutrient manipulation for highly improved in vitro plant regeneration in Finger Millet- Elevsiae corocana (L.) Gaerth. In Vitro Cell Dev Biol Plant 40:515–519CrossRefGoogle Scholar
  30. Kovacs E, Nyitrai P, Czovek P, Ovari M, Keresztes A (2009) Investigation into the mechanism of the phosphoinositide signalling pathway in the anti-senescence effect of low- concentration stressors on detached barley leaves. Plant Biol 9:420–426Google Scholar
  31. Mackinney G (1941) Absorption of light by chlorophyll solution. J Biol Chem 140:315–322Google Scholar
  32. Maiti RK, Pinero JLH, Oreja JG, Santiago DL (2004) Plant based bioremediation and mechanisms of heavy metal tolerance of plants: a review. Proc Indian Natn Sci Acad B 70:1–12Google Scholar
  33. Mamun ANK, Matin MN, Bari MA, Siddique NA, Sultana RS, Rahman MH, Musa ASM (2004) Micropropagation of woody legume (Albizia lebbeck) through tissue culture. Pak J Bio Sci 7:1099–1103CrossRefGoogle Scholar
  34. Marschner H (1995) Mineral nutrition of higher plants. Academic press, CambridgeGoogle Scholar
  35. Mohan BS, Hosetti BB (2006) Phytotoxicity of cadmium on the physiological dynamics of Salvinia natans L. grown in macrophyte ponds. J Environ Boil 27:701–704Google Scholar
  36. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiol Plant 15:473–497CrossRefGoogle Scholar
  37. Nehnevajova E, Herzig R, Erismann KH, Schwitzguebel JP (2007) In vitro breeding of Brassica juncea L. To enhance metal accumulation and extraction properties. Plant Cell Rep 26:429–437PubMedCrossRefGoogle Scholar
  38. Nyitrai P, Mayer M, Keresztes O (2007) Involvement of the phosphoinositide signaling pathway in anti senescence effect of low concentration stressors on detached barley leaves. Plant Biol 9:420–426PubMedCrossRefGoogle Scholar
  39. Oncel I, Kele Y, Ustun AS (2000) Interactive effects of temperature and heavy metal stress on the growth and some biochemical compounds in wheat seedlings. Environ Poll 107:315–320CrossRefGoogle Scholar
  40. Perveen S, Varshney A, Anis M (2011) Influence of cytokinins, basal media and pH on adventitious shoot regeneration from excised root cultures of Albizia lebbeck L. J For Res 22:47–52CrossRefGoogle Scholar
  41. Prasad MNV, Malee P, Waloszek A, Bajko M, Strzalka K (2001) Physiological responses of Lemna trisulca L. (duckweed) to cadmium and copper bioaccumulation. Plant Sci 161:881–889CrossRefGoogle Scholar
  42. Purnhauser L, Gyalai G (1993) Effect of copper on shoot and root regeneration in wheat, triticale, and rape and tobacco tissue cultures. Plant Cell Tiss Org Cult 35:131–139CrossRefGoogle Scholar
  43. Rabier J, Prudent P, Szymanska B, Mevy JP (2003) Metal accumulating plants: mediums role. J de Physique Archives IV France 107:1115–1118CrossRefGoogle Scholar
  44. Ratsch G, Christian K (2004) Enzyklopadie der psychoktiven Pflanzen, Botanik, Ethnopharmakologie and Anwendungen, 17th edn. Springer, BerlinGoogle Scholar
  45. Rosa GDL, Videa JRP, Montes M, Parsons JG, Aguilera IC, Gardea-Torresdey JL (2004) Cadmium uptake and translocation in Tumble weed (Salsola kali), a potential Cd- hyper accumulator desert plant species: ICP/OES and XAS studies. Chemosphere 55:1159–1168PubMedCrossRefGoogle Scholar
  46. Rout GR, Samantaray S, Das P (1999) In vitro selection and biochemical characterisation of zinc and manganese adapted callus lines in Brassica spp. Plant Sci 146:89–100CrossRefGoogle Scholar
  47. Sharma SS, Dietz KJ (2006) The significance of amino acids and acid-derived molecules in plant responses and adaptation to heavy metal stress. J Exp Bot 57:711–726PubMedCrossRefGoogle Scholar
  48. Sharmila P, Pardha Saradhi P (2002) Proline accumulation in heavy metal stressed plants: an adaptive strategy. In: Prasad MNV, Strzalka K (eds) Physiology and biochemistry of metal toxicity and tolerance in plants. Kluwer, Dordrecht, pp 179–200Google Scholar
  49. Singh AN, Zeng DN, Chen FS (2005) Heavy metal concentrations in redeveloping soil of mine spoil under plantations of certain native woody species in dry tropical environment. India J Environ Sci 20:168–174Google Scholar
  50. Spirochova IK, Punchocharova J, Kafka Z, Kubal M, Soudek P, Vevek T (2003) Accumulation of heavy metals by in vitro cultures of plants. Water Air Soil Poll Focus 3:269–276CrossRefGoogle Scholar
  51. Srivastava PS, Ali G, Iqbal M, Narula A, Bharti N (2002) Micropropagation of Bacopa and effect of heavy metals on growth performance. In: Nandi SK, Palni LMS, Kumar A (eds) Role of plant tissue culture in biodiversity conservation and economic development. Gyanodaya Prakashan, Nanital, pp 325–344Google Scholar
  52. Sugla T, Purkayastha J, Singh SK (2007) Micropropagation of Pongamia pinnata through enhanced axillary branching. In Vitro Cell Dev Biol Plant 43:409–414CrossRefGoogle Scholar
  53. Szafranska K, Cvikrova M, Kowalska U, Gorecka K, Gorecki R, Martincova O, Janas KM (2010) Influence of copper ions on growth, lipid peroxidation, and proline and polyamines content in carrot rosettes obtained from anther culture. doi: 10.1007/s11738-010-0610y
  54. Tantrey MS, Agnihotry RK (2010) Chlorophyll and proline content of Gram (Cicer arietinum L.) under cadmium and mercury treatments. Res J Agri Sci 1:119–122Google Scholar
  55. R Development Core Team (2011) R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. ISBN 3-900051-07-0, URL http://www.R-project.org/
  56. Vankatramany P (1968) Silviculture of genus Albizia and species. Silviculture of Indian trees. No. 22. Government of India, Delhi, p 54Google Scholar
  57. Welch RM (1995) Micronutrient nutrition of plants. Crit Rev Plant Sci 14:49–87Google Scholar
  58. Xu J, Yin H, Li X (2009) Protective effect of proline against cadmium toxicity in micropropagated hyperaccumulator, Solanum nigrum. Plant Cell Rep 28:325–333PubMedCrossRefGoogle Scholar
  59. Zengin FK, Kirbag S (2007) Effect of copper on chlorophyll, proline and abcisic acid level of Sunflower (Helianthus annuus L.) seedlings. J Environ Biol 28:561–566PubMedGoogle Scholar
  60. Zenk MH (1996) Heavy meal detoxification in higher plants- a review. Gene 179:21–30PubMedCrossRefGoogle Scholar
  61. Zhang-Pei, Zhou Qin, Jiang-Haidong (2007) Alleviate effects of exogenous ascorbate acid on Cd stress of rape seedlings. Plant Physiol 46:73–77Google Scholar

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  1. 1.Plant Biotechnology Laboratory, Department of BotanyAligarh Muslim UniversityAligarhIndia
  2. 2.Department of Plant Production, College of Food and Agricultural SciencesKing Saud UniversityRiyadhSaudi Arabia

Personalised recommendations