Abstract
5 BB grapevine rootstock cuttings were utilized to create the explants used in the experiment. The one-node micro-cuttings were used in the experiment. The sterilized explants were planted in Murashige and Skoog (MS) basic nutrient medium with 1 mg L–1 6‑benzylaminopurine (BAP). After 2 weeks of planting, the shoots were transferred to MS nutrient medium in which 1 mg L−1 indole-3-butyric acid (IBA) for rooting and elongation of shoot. After 3 weeks, the shoots were obtained by cutting 2–3 cm from the top of the elongated shoots and were transferred to MS medium containing 1 mg L−1 IBA containing different doses of sorbitol (0 M, 0.2 M, 0.4 M) and salicylic acid (SA) (0 mM, 0.5 mM, 1 mM and 2 mM) to examine their rooting and drought responses. After drought and different doses of SA applications, some parameters of shoot growth and physiological features were determined. According to the overall findings of the evaluated properties, the 0.2 M dose of sorbitol produced more effective drought stress in this study as compared with the other tested doses. According to this study, adding 2 mM SA to the nutritive medium had a positive impact on reducing the adverse effects of drought stress, degree of damage (1–4), tolerance rate, ion flux (%), rate of cell membrane damage (%), and proportional water content (%). It was determined that the negative effect of drought stress on the plants was reduced with the application of 1 and 2 mM SA and the highest sorbitol dose.
Similar content being viewed by others
References
Abid M, Ali S, Qi LK, Zahoor R, Tian Z, Jiang D, Snider JL, Dai T (2018) Physiological and biochemical changes during drought and recovery periods at tillering and jointing stages in wheat (Triticum aestivum L.). Sci Rep 8(1):4615. https://doi.org/10.1038/s41598-018-21441-7
Ahmad A, Aslam Z, Naz M, Hussain S, Javed T, Aslam S, Raza A, Hayssam MA, Siddiqui MH, Salem MZM, Hano C, Shabbir R, Ahmar S, Saeed T, Jamal MA (2021) Exogenous salicylic acid-induced drought stress tolerance in wheat (Triticum aestivum L.) grown under hydroponic culture. PLoS ONE 17(6):e270729. https://doi.org/10.1371/journal.pone.0270729
Asgher M, Khan MIR, Anjum NA, Khan NA (2015) Minimizing toxicity of cadmium in plants—Role of plant growth regulators. Protoplasma 252:399–413. https://doi.org/10.1007/s00709-014-0710-4
Bidabadi SS, Ghobadi C, Baninasab B (2012) Influence of salicylic acid on morphological and physiological responses of banana (’Musa acuminata’ cv. ‘Berangan’, AAA) shoot tips to in vitro water stress induced by polyethylene glycol. Plant Omics 5(1):33–39
Bilir Ekbiç H (2017) Effects of different salicylic acid doses on salt tolerance of American vine rootstocks. Bangladesh J Bot 46(2):639–645
Bilir Ekbic H, Gecene I, Ekbic E (2022) Determination of the tolerance of fox Grapes (Vitis labrusca L.) to drought stress by PEG application in vitro. Erwerbs-Obstbau 64:87–94. https://doi.org/10.1007/s10341-022-00669-8
Brito G, Costa A, Fonseca HMAC, Santos CV (2003) Response of Olea europaea ssp. maderensis in vitro shoots exposed to osmotic stress. Sci Hortic 97(3–4):411–417. https://doi.org/10.1016/S0304-4238(02)00216-9
Bundig C, Blume C, Peterha C, Winkelmann T (2016) Changed composition of metabolites in Solanum tuberosum subjected to osmotic stress in vitro: Is sorbitol taken up? Plant Cell Tiss Organ Cult 127:195–206. https://doi.org/10.1007/s11240-016-1042-1
Chaves MM, Zarrouk O, Francisco R, Costa JM, Santos T, Regalado AP, Rodrigues ML, Lopes CM (2010) Grapevine under deficit irrigation: hints from physiological and molecular data. Ann Bot 105:661–676. https://doi.org/10.1093/aob/mcq030
Farooq M, Basra SMA, Wahid A, Ahmad N, Saleem BA (2009a) Improving the drought tolerance in rice (Oryza sativa L.) by exogenous application of salicylic acid. J Agron Crop Sci 195:237–246. https://doi.org/10.1111/j.1439-037X.2009.00365.x
Gambetta GA, Herrera JC, Dayer S, Feng Q, Hochberg U, Castellarin SD (2020) The physiology of drought stress in grapevine: towards an integrative definition of drought tolerance. J Exp Bot 71(16):4658–4676. https://doi.org/10.1093/jxb/eraa245
Gangopadhyay G, Basu S, Gupta S (1997) In vitro selection and physiological characterization of NaCl- and mannitol-adapted callus lines in Brassica juncea. Plant Cell Tissue Organ Cult 50:161–169
Gopal J, Iwama K (2007) In vitro screening of potato against water-stress mediated through sorbitol and polyethylene glycol. Plant Cell Rep 26:693–700. https://doi.org/10.1007/s00299-006-0275-6
Hayat Q, Hayat S, Irfan M, Ahmad A (2010) Effect of exogenous salicylic acid under changing environment: a review. Environ Exp Bot 68:14–25. https://doi.org/10.1016/j.envexpbot.2009.08.005
Hayat S, Hasan SA, Fariduddin Q, Ahmad A (2008) Growth of tomato (Lycopersicon esculentum) in response to salicylic acid under water stress. J Plant Interact 3(4):297–304. https://doi.org/10.1080/17429140802320797
Jamali B, Eshghi S, Tafazoli E (2011) Vegetative and reproductive growth of strawberry plants cv. ‘Pajaro’ affected by salicylic acid and nickel. J Agric Sci Technol 13:895–904
Khalvandi M, Siosemardeh A, Roohi E, Keramati S (2021) Salicylic acid alleviated the effect of drought stress on photosynthetic characteristics and leaf protein pattern in winter wheat. Heliyon 7(1):e5908. https://doi.org/10.1016/j.heliyon.2021.e05908
Khan MIR, Mehar F, Tasir SP, Anjum NA, Khan NA (2015) Salicylic acid-induced abiotic stress tolerance and underlying mechanisms in plants. Front Plant Sci 6:462. https://doi.org/10.3389/fpls.2015.00462
Kumlay AM, Eryiğit T (2011) Bitkilerde büyüme ve gelişmeyi düzenleyici maddeler: Bitki Hormonları. Iğdır Üni Fen Bilimleri Enst 1(2):47–56
Lawlor DW, Cornic G (2002) Photosynthetic carbon assimilation and associated metabolism in relation to water deficits in higher plants. Plant Cell Environ 25(2):275–294. https://doi.org/10.1046/j.0016-8025.2001.00814.x
Miura K, Tada Y (2014) Regulation of water, salinity, and cold stress responses by salicylic acid. Front Plant Sci 5(4):1–12. https://doi.org/10.3389/fpls.2014.00004
Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15(3):473–497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Nazar R, Umar S, Khan NA, Sareer O (2015) Salicylic acid supplementation improves photosynthesis and growth in mustard through changes in proline accumulation and ethylene formation under drought stress. S Afr J Bot 98:84–94. https://doi.org/10.1016/j.sajb.2015.02.005
Ordish G (1972) The great wine blight. Scribner, New York
Ozden M, Demirel U, Kahraman A (2009) Effects of proline on antioxidant system in leaves of grapevine (Vitis vinifera L.) exposed to oxidative stress by H2O2. Sci Hortic 119(2):163–168. https://doi.org/10.1016/j.scienta.2008.07.031
Patni B, Ansari S (2019) Role of exogenous application of salicylic acid on medicinal plants under drought stress: a review. J Stress Physiol Biochem 15(4):76–85
Placide R, Carpentier S, Swennen R (2012) Development of in vitro technique to screen for drought tolerant banana varieties by sorbitol induced osmotic stress. Afr J Plant Sci 6(15):416–425. https://doi.org/10.5897/AJPS12.101
Qaseem MF, Qureshi R, Shaheen H (2019) Effects of pre-anthesis drought, heat and their combination on the growth, yield and physiology of diverse wheat (Triticum aestivum L.) genotypes varying in sensitivity to heat and drought stress. Sci Rep 9(1):6955. https://doi.org/10.1038/s41598-019-43477-z
Rabeh MRM, Gadalla EG, Ahmed SA, Shehata MS (2021) Primitive effects of culture system, sorbitol and salicylic acid on growth of tissue culture derived date palm plants (Phoenix dactylifera L.) Amri cv. during acclimatization stage. Menoufia J Plant Prod 6(4):221–233. https://doi.org/10.21608/MJPPF.2021.169831
Rai MK, Kalia RK, Singh R, Gangola MP, Dhawan AK (2011) Developing stress tolerant plants through in vitro selection—An overview of the recent progress. Environ Exp Bot 71(1):89–98. https://doi.org/10.1016/j.envexpbot.2010.10.021
Sadeghipour O, Aghaei P (2012) Biochemical changes of common bean (Phaseolus vulgaris L.) to pretreatment with salicylic acid (SA) under water stress conditions. Int J Biosci IJB 2(8):14–22
Saruhan N, Saglam A, Kadioglu A (2011) Salicylic acid pretreatment induces drought tolerance and delays leaf rolling by inducing antioxidant systems in maize genotypes. Acta Physiol Plant 34(1):97–106. https://doi.org/10.1007/s11738-011-0808-7
Shirasu K, Nakajima H, Rajasekhar VK, Dixon RA, Lamb C (1997) Salicylic acid potentiates an agonist-dependent gain control that amplifies pathogen signals in the activation of defense mechanisms. Plant Cell 9(2):261–270
Sivritepe N, Erturk U, Yerlikaya C, Turkan I, Bor M, Ozdemir F (2008) Response of the cherry rootstock to water stress induced in vitro. Biol plant 52(3):573–576
Turhan E, Dardeniz A, Müftüoğlu NM (2005) Determining the tolerances to salinity stress of some american grapevine rootstocks. Bahçe 34(2):11–19
Vanhove AC, Vermaelen W, Panis B, Swennen R, Carpentier SC (2012) Screening the banana biodiversity for drought tolerance: can an in vitro growth model and proteomics be used as a tool to discover tolerant varieties and understand homeostasis. Front Plant Sci 3(176):1–10. https://doi.org/10.3389/fpls.2012.00176
Yamasaki S, Dillenburg LR (1999) Measurements of leaf relative water content in Araucaria angustifolia. Rev Bras Fisiol Veg 11(2):69–75
Yang C, Menz C, Fraga H, Costafreda-Aumedes S, Leolini L, Ramos MC, Molitorf D, Leeuwen C, Santos JA (2021) Assessing the grapevine crop water stress indicator over the flowering-veraison phase and the potential yield lose rate in important European wine regions. Agric Water Manag 261:107349. https://doi.org/10.1016/j.agwat.2021.107349
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
H. Bilir Ekbic and M. İlhan declare that they have no competing interests.
Additional information
Publisher’s Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
This article was presented in summary at the 4th International Cappadocia Scientific Research Congress (April 16–17, 2023, Nevsehir, Turkiye).
Rights and permissions
Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
About this article
Cite this article
Bilir Ekbic, H., İlhan, M. Effect of Different Salicylic Acid Doses on Kober 5 BB Grapevine Rootstock in Vitro Drought Stress Conditions. Applied Fruit Science 66, 649–656 (2024). https://doi.org/10.1007/s10341-023-00997-3
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10341-023-00997-3