Abstract
In vitro micropropagation is the most valid production method used for disease-free, clonal, and mass production of plants developed using unique breeding methods. This study was carried out to establish appropriate protocols for the micropropagation of 10 (Prunus amygdalus) × (P. amygdalus × P. persica) hybrid rootstocks obtained by crossbreeding. The maximum micro shoot and shoot length values were obtained from the medium (MED 11) as 3.92 shoots/explant and 14.59 mm, respectively. The best rooting values were obtained from MS medium supplemented with 1.75 mgL−1 IBA + 0.1 mgL−1 BAP (MED-5) in the rooting study. The highest average root number, root length, and root diameter of shoots 2.79 pcs/explant, 14.36 mm, and 1.15 pcs/explant, respectively, were observed. It is suggested that MS media supplemented with MED-11 (0.1 mg.L−1 IBA + 1.75 mg.L−1 BAP + 0.2 mg.L−1 GA3), MED-12 (0.1 mg.L−1 IBA + 2.0 mg.L−1 BAP + 0.2 mg.L−1 GA3) for regeneration, MED‑5 (1.75 mg.L−1 IBA + 0.1 mg.L−1 BAP), and MED‑6 (2.0 mg.L−1 IBA + 0.1 mg.L−1 BAP) hormone combinations for rooting would be appropriate for the micropropagation of hybrid rootstocks. It is thought that the results of the study can be a guide for new studies to be carried out especially in these species.
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Acknowledgements
The materials used in this study were obtained from the project supported by TUBITAK (Turkish Scientific and Technological Research Institution) (Project name: Evaluation of Prunus Hybrids Obtained by Interspecies Hybridization as Peach Rootstock. Project No: 217O024) apart from project method. For this reason, we would like thank to the Eastern Mediterranean Transitional Zone Agricultural Research Institute for their laboratory and chemical material support in this study.
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E. Bulunuz Palaz, R. Ugur and M. Yaman declare that they have no competing interests.
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Bulunuz Palaz, E., Ugur, R. & Yaman, M. Micropropagation Protocols of New Prunus Hybrids with Significant Rootstock Potential in Fruit Breeding and Cultivation. Erwerbs-Obstbau 65, 1359–1364 (2023). https://doi.org/10.1007/s10341-023-00908-6
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DOI: https://doi.org/10.1007/s10341-023-00908-6