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Integrated Management of Grape Gray Mold Disease Agent Botrytis cinerea in Vitro and Post-harvest

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Abstract

Gray mold caused by Botrytis cinerea is one of the most important diseases of fresh grapes in the field and during the post-harvest period. To control this disease, many fungicides are used. One of the sustainable solutions to replace chemical fungicides is using Trichoderma mycoparasite, medicinal plant essential oils, and new chemical fungicides. In the B. cinerea pathogenesis test, most of them showed the ability to cause disease, and among them the A91 isolate showed the highest pathogenicity. In a cross-culture experiment, most isolates of Trichoderma grew on the A91 isolate colony, and among them, the T39 isolate with 72.47% inhibition showed the highest inhibitory power. Regarding the effect of volatile metabolites, the maximum inhibition rate by the G2 isolate of the T. harzianum antagonist was 42.62%. Nordox fungicide showed the highest inhibition in vitro, with 95.99% inhibition of the pathogen. In the test of the effect of medicinal plant essential oils, the highest inhibition amount of 96.22% was seen for the essential oil of Trachyspermum with a concentration of 1000 mg/L. In cold storage conditions (5 °C), without wounding and wound formation, the highest inhibition of pathogenic fungal growth was achieved with 93.5% and 85%, respectively, by the combined treatment of Trichoderma and potassium phosphide. At a temperature of 20 °C, this compound showed the highest inhibition of B. cinerea A91 mycelium growth. This study showed that essential oils of medicinal plants and the combined application of Trichoderma and chemical fungicides could be the best way to control gray mold.

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Authors and Affiliations

  1. Department of Plant Protection, Faculty of Agriculture, University of Tehran, Tehran, Iran

    Alireza Rahmani

  2. Department of Horticulture, Faculty of Agriculture, University of Tehran, Tehran, Iran

    Yousef Hakimi

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  1. Alireza Rahmani
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Correspondence to Yousef Hakimi.

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A. Rahmani and Y. Hakimi declare that they have no competing interests.

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Rahmani, A., Hakimi, Y. Integrated Management of Grape Gray Mold Disease Agent Botrytis cinerea in Vitro and Post-harvest. Erwerbs-Obstbau 65, 1955–1964 (2023). https://doi.org/10.1007/s10341-022-00813-4

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