Skip to main content
SpringerLink
Log in

Antioxidant and Antimicrobial Potential of Wild Chestnut Ohrid Diamond Extract—An Opportunity for Creation of New Natural Products

Erwerbs-Obstbau volume 65, pages 83–91 (2023)Cite this article

Abstract

The aim of this research was to prepare aqueous and ethanolic extracts of the seed and leaf of the wild chestnut known as Ohrid diamond and to determine the antioxidant and antimicrobial activity. In general, ethanolic extracts showed higher antioxidant potential compared with the aqueous extracts. Moreover, ethanolic seed extracts had better results in all of the antioxidant tests compared with the ethanolic extracts from the leaves. The ethanolic seed extract was characterized by a statistically significant (p < 0.05) lower IC50 value for the ability to capture DPPH radicals (0.63 mg/ml) compared with all other tested extracts. Furthermore, the extracts showed statistically significantly (p < 0.05) higher antimicrobial activity against five tested bacteria (Staphylococcus aureusATCC25923, Listeria monocytogenesATCC19115, Escherichia coliATCC11230, Shigella sonneiATCC29930, Pseudomonas aeruginosaATCC35554) compared to tetracycline and chloramphenicol. Given the good biological activity that was established, this research can serve as a novel starting point to contribute to greater utilization of this plant in the pharmaceutical, cosmetic, and food industries, and the obtained extracts could substitute for the numerous synthetic additives that are regularly used in these industries.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

USD 39.95

Price includes VAT (Canada)

Fig. 1
Fig. 2
Fig. 3
Fig. 4

References

  • Antov MG, Dordevic TR (2017) Environmental-friendly technologies for the production of antioxidant xylooligosaccharides from wheat chaff. Food Chem 235:175–180. https://doi.org/10.1016/j.foodchem.2017.05.058

    Article  CAS  Google Scholar 

  • Barreira JCM, Ferreira ICFR, Oliveira MBPP, Pereira JA (2008) Antioxidant activities of the extracts from chestnut flower, leaf, skins and fruit. Food Chem 107:1106–1113. https://doi.org/10.1016/j.foodchem.2007.09.030

    Article  CAS  Google Scholar 

  • Bonomo MG, Cafaro C, Russo D, Calabrone L, Milella L, Saturnino C, Capasso A, Salzano G (2018) Antimicrobial Activity, Antioxidant Properties and Phytochemical Screening of Aesculus hippocastanum Mother Tincture against Food-borne Bacteria. Lett Drug Des Discov 17:48–56. https://doi.org/10.2174/1570180816666181108114115

    Article  CAS  Google Scholar 

  • Brand-Williams, W., Cuvelier, M. E., & Berset, C. (1995). Use of a free radical method to evaluate antioxidant activity. LWT-Food Science and Technology, 28, 25–30.

  • Celep AGS, Yilmaz S, Coruh N (2012) Antioxidant Capacity and Cytotoxicity of Aesculus hippocastanum on Breast Cancer MCF‑7 Cells. J Food Drug Anal 20(3):692–698. https://doi.org/10.6227/jfda.2012200318

    Article  Google Scholar 

  • Dahash SL, Abass OK, Abdul-Razaq MM, Al-Kuraishy HM, Al-Gareeb AI (2021) Aesculus hippocastanum-Derived extract β‑Aescin and in vitro antibacterial activity. J Microsc Ultrastruct 9:26–30. https://doi.org/10.4103/JMAU.JMAU_56_19

    Article  Google Scholar 

  • Dinis TCP, Madeira VMC, Almeida LM (1994) Action of phenolic derivatives (acetaminophen, salicylate, and 5‑amino salicylate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Arch Biochem Biophys 315:161–169

    Article  CAS  Google Scholar 

  • Ferreira, I. C. F. R., Baptista, P., Vilas-Boas, M., & Barros, L. (2007). Freeradical scavenging capacity and reducing power of wild edible mushrooms from northeast Portugal: Individual cap and stipe activity. Food Chemistry, 100, 1511–1516. https://doi.org/10.1016/j.foodc hem.2005.11.043

  • Fujimura T, Tsukahara K, Moriwaki S, Hotta M, Kitahara T, Takema Y (2006) A horse chestnut extract, which induces contraction forces in fibroblasts, is a potent anti-aging ingredient. J Cosm Sci 29:369–376

    Google Scholar 

  • Harborne JB, Baxter H (1993) Phytochemical dictionary. Taylor and Francis, London.

    Google Scholar 

  • Hu M, Yang X, Chang X (2021) Bioactive phenolic components and potential health effects of chestnut shell: A review. J Food Biochem 45:e13696. https://doi.org/10.1111/jfbc.1369

    Article  CAS  Google Scholar 

  • Jung BS, Lee NK, Na DS, Yu HH, Paik HD (2016) Comparative analysis of the antioxidant and anticancer activities of chestnut inner shell extracts prepared with various solvents. J Sci Food Agric 96(6):2097–2102. https://doi.org/10.1002/jsfa.7324

    Article  CAS  Google Scholar 

  • Klaus A, Kozarski M, Niksic M, Jakovljevic D, Todorovic N, VanGriensven LJLD (2011) Antioxidative activities and chemical characteriyation of polysaccharides extracted from the basidiomycete Schizophyllum commune. LWT. Food Sci Technol 30:1–7. https://doi.org/10.1016/j.lwt.2011.05.010

    Article  CAS  Google Scholar 

  • Klaus A, Kozarski M, Vunduk J, Todorovic N, Jakovljevic D, Zizak Z, Pavlovic V, Levic S, Niksic M, Van Griensven LJLD (2015) Biological potential of extracts of wild edible Basidiomycete mushroom Grifola forndosa. Food Res Int 67:272–283. https://doi.org/10.1016/j.foodres.2014.11.035

    Article  CAS  Google Scholar 

  • Kozarski M, Klaus A, Nikšić M, Jakovljević D, Helsper JPFG, Van-Griensven LJLD (2011) Antioxidative and immunomodulating activities of polysaccharide extracts of the medicinal mushrooms Agaricus bisporus, Agaricus brasiliensis, Ganoderma lucidum and Phellinus linteus. Food Chem 129:1667–1675. https://doi.org/10.1016/j.foodchem.2011.06.029

    Article  CAS  Google Scholar 

  • Kędzia R, Lis MW (2021) Assessment of the antibacterial activity of chestnut (Castanea sativa) and cloves (Syzygium aromaticum) herbal extracts as an alternative to antibiotics use during post-hatching period of chicks. Sci Technol Innov 11(4):48–54

    Article  Google Scholar 

  • McDaniel DH, Neudecker BA, DiNardo JC, Lewis JA, Maibach HI (2005) Idebenone: A new antioxidant—Part I. Relative assessment of oxidative stress protection capacity compared to commonly known antioxidants. J Cosmet Dermatol 4(1):10–17. https://doi.org/10.1111/j.1473-2165.2005.00152.x

    Article  CAS  Google Scholar 

  • Moure A, Cruz JM, Franco D, Domínguez JM, Sineiro J, Domínguez H, José Núñez M, Parajó JC (2001). Natural antioxidants from residual sources. Food Chemistry, 72(2), 145–171. https://doi.org/10.1016/S0308-8146(00)00223‑5

  • Ribeiro A, Ruphuy G, Lopes JC, Dias MM, Barros L, Barreiro F, Ferreira ICFR (2015) Spray-drying microencapsulation of synergistic antioxidant mushroom extracts and their use as functional food ingredients. Food Chem 188:612–618. https://doi.org/10.1016/j.foodchem.2015.05.061

    Article  CAS  Google Scholar 

  • Scherer R, Godoy HT (2009) Antioxidant activity index (AAI) by the 2,2-diphenyl-1-picrylhydrazyl method. Food Chem 112:654–658. https://doi.org/10.1016/j.foodchem.2008.06.026

    Article  CAS  Google Scholar 

  • Sood S, Mishra M, Sood A, Thakur V (2015) Hypoglycaemic and hypocholesterolimic efficacy of horse chestnut (Aesculus indica) using rat models. J Clin Nutr Diet 1(1):1–8

    Article  Google Scholar 

  • Squillaci G, Apone F, Sena LM, Carola A, Tito A, Bimonte M, Lucia AD, Colucci G, Cara FL, Morana A (2018) Chestnut (Castanea sativa Mill.) industrial wastes as a valued bioresource for the production of active ingredients. Process Biochem 64:228–236. https://doi.org/10.1016/j.procbio.2017.09.017

    Article  CAS  Google Scholar 

  • Stojanova M, Pantić M, Karadelev M, Čuleva B, Nikšić M (2021a) Antioxidant potential of extracts of three mushroom species collected from the Republic of North Macedonia. J Food Process Preserv 45(2):e15155. https://doi.org/10.1111/jfpp.15155

    Article  CAS  Google Scholar 

  • Stojanova M, Djukic D, Moracanin SV, Stojanova MT, Mladenoska I, Bektashi NL (2021b) Determination of antioxidant potential of Agaricus macrosporus and Russula vesca mushroom extract. AGBIOL, Proceedings of III International Agricultural, Biological & Life. Science, Conference, Trakya University, Turkey, pp 400–407

    Google Scholar 

  • Stojanova TM (2019) Soil and Plant Testing Manual for Agrochemical. Analysis (Academic Press, Skopje). ISBN 978-608-231-278-1. COBISS.MK-ID 110378250

  • Sławińska A, Radzki W, Kalbarczyk J (2013) Antioxid Activities Polyphenolics Content Flammulina Velutipes Mushroom Extr Kerla Polonica De Gruyter 59(3):26–36. https://doi.org/10.2478/hepo-2013-0014

    Article  Google Scholar 

  • Takahashi T, Tsurunaga Y, Schmidt WF, Yoshino K (2017) Functional evaluation of horse chestnut seed and its application in the production of compounded paper for effective utilization of an untapped resource. J Wood Sci 63:484–495. https://doi.org/10.1007/s10086-017-1649-9

    Article  CAS  Google Scholar 

  • Tuyen P, Xuan T, Khang DO, Ahmad A, Quan N, Anh TT, Anh LA, Minh T (2017) Phenolic compositions and antioxidant properties in bark, flower, inner skin, kernel and leaf extracts of Castanea crenata Sieb. et Zucc. Antioxidants (basel) 6(2):31. https://doi.org/10.3390/antiox6020031

    Article  CAS  Google Scholar 

  • Vella, F. M., Laratta, B., La Cara, F., & Morana, A. (2018). Recovery of bioactive molecules from chestnut (Castanea sativa Mill.) byproducts through extraction by different solvents. Natural Product Research, 32(9), 1022–1032. https://doi.org/10.1080/14786 419.2017.1378199

  • Vidović S, Zoran-Zeković Z, Mujić I, Lepojević Ž, Radojković M, Živković J (2011) The antioxidant properties of polypore mushroom Daedaleopsis confragosa. Cent Eur J Biol 6(4):575–582. https://doi.org/10.2478/s11535-011-0029-5

    Article  Google Scholar 

  • Yuriy KK, Arsene MMJ, Aliya MV, Podoprigora IV, Volina EG (2021) Assessment of antimicrobial activity of ethanolic and aqueous extracts of Aesculus hippocastanum L. (horse chestnut) bark against bacteria isolated from urine of patients diagnosed positive to urinary tract infections. doi https://doi.org/10.1101/2021.08.23.457193

    Book  Google Scholar 

  • Zhang Y, Yang Z, Liu G, Wu Y, Ouyang J (2020) Inhibitory effect of chestnut (Castanea mollissima Blume) inner skin extract on the activity of alpha-amylase, alpha-glucosidase, dipeptidyl peptidase IV and in vitro digestibility of starches. Food Chem 324:126847. https://doi.org/10.1016/j.foodchem.2020.126847

    Article  CAS  Google Scholar 

  • Zhang Z, Li S, Lian XY (2010) An Overview of Genus Aesculus L.: Ethnobotany, Phytochemistry, and Pharmacological Activities. Pharmaceutical. Crops, vol 1, pp 24–51 https://doi.org/10.2174/2210290601001010024

    Book  Google Scholar 

  • Şeker GK (2019) Horse Chestnut. Chapter 3.26, Nonvitamin and Nonmineral Nutritional. Supplements. https://doi.org/10.1016/B978-0-12-812491-8.00042-4

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Faculty of Agricultural Sciences and Food, University of Ss. Cyril and Methodius, Skopje, North Macedonia

    Monika Stojanova & Marina Todor Stojanova

  2. Faculty of Agronomy Čačak, University of Kragujevac, Čačak, Serbia

    Dragutin Djukic

  3. Agriculture Faculty, Erciyes University, Kayseri, Turkey

    Aziz Şatana

  4. Faculty of Agriculture, University of Belgrade, Belgrade, Serbia

    Blazo Lalevic

Authors
  1. Monika Stojanova
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Dragutin Djukic
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Marina Todor Stojanova
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Aziz Şatana
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Blazo Lalevic
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

Conceptualization, investigation, formal analysis, writing of original draft: Monika Stojanova; data curation, investigation: Dragutin Djukic; investigation, methodology, formal analysis, editing of original draft: Marina Todor Stojanova; data curation: Aziz Satana; data curation, investigation: Blazo Lalevic.

Corresponding author

Correspondence to Monika Stojanova.

Ethics declarations

Conflict of interest

M. Stojanova, D. Djukic, M. T. Stojanova, A. Şatana, and B. Lalevic declare that they have no competing interests.

Ethical standards

For this article no studies with human participants or animals were performed by any of the authors. All studies mentioned were in accordance with the ethical standards indicated in each case.

Additional information

Data Availability Statement

Data related to this article can be found in this study.

Rights and permissions

Reprints and Permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Stojanova, M., Djukic, D., Stojanova, M.T. et al. Antioxidant and Antimicrobial Potential of Wild Chestnut Ohrid Diamond Extract—An Opportunity for Creation of New Natural Products. Erwerbs-Obstbau 65, 83–91 (2023). https://doi.org/10.1007/s10341-022-00668-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10341-022-00668-9

Keywords

  • Wild chestnut
  • Ohrid diamond
  • Extract
  • Antimicrobial activity
  • Antioxidant activity

Search

Navigation