Skip to main content

Advertisement

Log in

Genetic Characterization and Conservation of Grapevine Germplasms in Kayseri Province of Turkey

Genetische Charakterisierung und Erhaltung genetischer Ressourcen von Weinreben in der türkischen Provinz Kayseri

  • Original Article / Originalbeitrag
  • Published:
Erwerbs-Obstbau Aims and scope Submit manuscript

Abstract

Turkey is within the region where the viticulture has been traditionally practiced since ancient times. The Cappadocia location, including the province of Kayseri, is an important grape production area with its autochthonous grape cultivars and unique tradition in history. Due to viticulture history, this region is rich in vine genetic resources. This survey study was carried out in vineyards areas and residential gardens in 23 different regions of Kayseri province between 2017 and 2019. A total of 174 local genotypes from different locations were determined and were further examined through molecular characterization with SSR markers. A total of 112 bands were amplified by 12 SSR primers, all of which were polymorphic with a 100% polymorphism. The highest number of polymorphic bands (18) was produced by the primer Scu8vv, while the lowest number of polymorphic bands (3) was found in VMC8D3 and VMC8E6. An UPGMA dendrogram was created via scoring the bands and the genetic similarity between the genotypes was determined between 0.63–1.0. In conclusion, a wide range of genetic diversity was determined in grapes of Kayseri indicating an ancient residential germplasm collection that could be used for breeding studies.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Aslantaş Ş (2010) Molecular characterization of western Mediterranean grape varieties and their genetic relationship with country vine sources. Master’s Thesis. Ankara University, Biotechnology Institute, Ankara, p 55 (in Turkish)

    Google Scholar 

  • Benito A, Muñoz-Organero G, Deandrés MT, Ocete R, García-Muñoz S, López MÁ, Arroyo-García R, Cabello F (2016) Ex situ ampelographical characterization of wild Vitis vinifera from fifty-one Spanish populations. Aust J Grape Wine Res 23:143–152

    Article  Google Scholar 

  • Boz Y, Bakır M, Çelikkol BP, Kazan K, Yılmaz F, Çakır B, Aslantaş Ş, Söylemezoğlu G, Yaşasın AS, Özer C, Çelik H, Ergül A (2011) Genetic characterization of grape (Vitis vinifera L.) germplasm from Southeast Anatolia by SSR markers. Vitis 50(3):99–106

    Google Scholar 

  • Dice LR (1945) Measures of the amount of ecologic association between species. Ecology 26:297–302

    Article  Google Scholar 

  • Dilli Y (2008) Studies on characterization of some important grape varieties, types and clones in the Aegean region with microsatellite (SSR) markers. PhD Thesis. Ege University, Institute of Science, İzmir, p 96 (in Turkish)

    Google Scholar 

  • Dong Z, Liu W, Li X, Tan W, Zhao Q, Wang M, Ren R, Ma X, Tang X (2018) Genetic relationships of 34 grapevine varieties and construction of molecular fingerprints by SSR markers. Biotechnol Biotechnol Equip 32(4):942–950

    Article  Google Scholar 

  • Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus (Madison) 12:13–15

    Google Scholar 

  • Ferlito F, Nicolosi E, La Malfa S, Cicala A, Gentile A (2018) First characterization of minor and neglected Vitis vinifera L. cultivars from Mount Etna. Hort Sci (Prague) 45(1):37–46

    Article  CAS  Google Scholar 

  • Garcia-Muñoz S, Muñoz-Organero G, De Andrés MT, Cabello F (2011) Ampelography—an old technique with future uses: the case of minor varieties of Vitis vinifera L. from the Balearic Islands. J Int Sci Vigne Vin 45(3):125–137

    Google Scholar 

  • Halász G, Veres A, Kozma P, Kıss E, Balogh A, Gallı Z, Szőke A, Hoffmann S, Heszk L (2005) Microsatellite fingerprinting of grapevine (Vitis vinifera L.) varieties of the Carpathian basin. Vitis 44(4):173–180

    Google Scholar 

  • Karagöz A, Tan A, Özbek K, Yıldız A, Keskin E, Bilgin A, Aykas L, Deniz D (2020) Current situation and future in the field of plant genetic resources in agriculture. Turkey Agricultural Engineering IX. Technical Congress Proceedings Book-Ankara,Turkey. (in Turkish)

    Google Scholar 

  • Knezović Z, Mandić A, Perić N, Beljo J, Mihaljević MJ (2017) Morphological and genetic characterization of vine grape cultivars of Herzegovina. Croat Rev Econ Bus Soc Stat 3(2):1–9

    Article  Google Scholar 

  • Marak CK, Laskar MA (2010) Analysis of phenetic relationship between citrus indica Tanaka and a few commercially important citrus species by ISSR markers. Sci Hortic 124:345–348

    Article  CAS  Google Scholar 

  • Muganu M, Dangl G, Maui Aradhya M, Frediani M, Scossa A, Stover A (2009) Ampelographic and DNA characterization of local grapevine accessions of the Tuscia area (Latium, Italy). Am J Enol Vitic 60(1):110–115

    CAS  Google Scholar 

  • Peakall R, Smouse PE (2012) GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research-an update. Bioinformatics 28:2537–2539. https://doi.org/10.1093/bioinformatics/bts460

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Popescu CF, Maul E, Dejeu LC, Dinu D, Gheorge RN, Laucou V, Lacombe T, Migliaro D, Crespan M (2017) Identification and characterization of Romanian grapevine genetic resources. Vitis 56:173–180

    Google Scholar 

  • Rohlf FJ (2000) NTSYS-pc, numerical taxonomy and multivarite analysis, system, version 2.11. Exeter Setauket, New York

    Google Scholar 

  • Sabir A (2008) Ampelographic and molecular characterization of some grape varieties and rootstocks. PhD Thesis. Çukurova University, Institute of Science, Adana, p 154 (in Turkish)

    Google Scholar 

  • Sabir A, Ikten H, Mutlu N, Sari D (2018) Genetic identification and conservation of local Turkish grapevine (Vitis vinifera L.) genotypes on the edge of extinction. Erwerbs-Obstbau 60:31–38

    Article  Google Scholar 

  • Scholz M, Selbig J (2006) Visualization and analysis of molecular data. In: Weckwerth W (ed) Metabolomics: methods and protocols. Methods in molecular biology, vol 358. Humana Press, New York, pp 87–104

    Chapter  Google Scholar 

  • Tan A (2010) Turkey plant genetic resources and conservation. Anadolu J AARI 20(1):9–37 (in Turkish)

    Google Scholar 

  • Tekdal D, Sarlar S (2016) Local grapevine genetic resources and importance. Vineyard Gard Sci J 3(3):19–25 (in Turkish)

    Google Scholar 

  • This P, Jung A, Boccacci P, Borrego J, Botta R, Costantini L, Crespan M, Dangl GS, Eisenheld C, Ferreiramonteiro F, Grando S, Ibanez J, Lacombe T, Laucou V, Magalhaes R, Meredith CP, Milani N, Peterlunger E, Regner F, Zulini L, Maul E (2004) Development of a standard set of microsatellite reference alleles for identification of grape. Theor Appl Genet 109(7):1448–1458

    Article  CAS  Google Scholar 

  • Uzun A (2009) Characterization of genetic diversity in citrus fruits with SRAP markers. PhD Thesis. Çukurova University, Institute of Science, Adana, p 369 (in Turkish)

    Google Scholar 

  • Yüksel C (2008) SSRs (simple sequence repeats) based genetic characterization of Manisa, İzmir, Muğla and Kütahya grapevine germplasms. PhD Thesis. Ankara University, Institute of Science, Ankara, p 59 (in Turkish)

    Google Scholar 

  • Yıldırım N (2010) Characterization of black grape groups based on SSR (simple sequence repeat) markers and their genetic relationship with country grapevine sources. Master’s Thesis. Ankara University, Biotechnology Institute, Ankara, p 71 (in Turkish)

    Google Scholar 

  • Yılmaz N, Alas T, Abak K (2012) Collection of local vegetables genotypes with their wild relatives in North Cyprus. Acta Hortic 960:135–138

    Article  Google Scholar 

Download references

Acknowledgements

This study was supported by Erciyes University Scientific Research Projects Unit with the project number FDK-2018-8048.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Aydın Uzun.

Ethics declarations

Conflict of interest

G. Yılmaz, A. Uzun, A. Sabır and H. Pınar declare that they have no competing interests.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Yılmaz, G., Uzun, A., Sabır, A. et al. Genetic Characterization and Conservation of Grapevine Germplasms in Kayseri Province of Turkey. Erwerbs-Obstbau 64 (Suppl 1), 55–63 (2022). https://doi.org/10.1007/s10341-022-00665-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10341-022-00665-y

Keywords

Schlüsselwörter

Navigation