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Transcriptomic and metabolomic reprogramming in cotton after Apolygus lucorum feeding implicated in enhancing recruitment of the parasitoid Peristenus spretus

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Abstract

Different types of insect feeding can activate distinct plant resistance mechanisms and trigger the generation of specific volatile compounds. Transcriptomic changes and the genomic basis underlying plant defense in response to chewing herbivores or phloem-feeding insects have been relatively well investigated, while better insight into molecular mechanisms underlying the plant defense response, in particular, volatile emissions triggered by sap-feeding insects such as the green plant bug Apolygus lucorum is needed. Here, we monitored transcriptomic and volatile metabolomic changes in cotton over time during A. lucorum infestation. RNA-seq analysis showed that 1614 transcripts were differentially expressed (log2|Ratio| ≥ 1; q ≤ 0.05) in cotton leaves infested by A. lucorum. All differentially expressed genes (DEGs) in jasmonic acid (JA; 48 genes) and salicylic acid (SA; 5 genes) pathways were upregulated, highlighting a central role of JA in A. lucorum-induced signaling without attenuating the SA pathway. Moreover, all DEGs (30 genes) involved in herbivore-induced volatile biosynthesis were upregulated. Consistently, A. lucorum-induced cotton volatile blends and synthetic methyl salicylate significantly attracted the parasitoid Peristenus spretus. The present data indicated that cotton plants after A. lucorum infestation undergo rapid, extensive transcriptome reprogramming mediated by complex signaling networks in which the JA and SA pathways act synergistically, leading to a specific volatile profile involved in an indirect plant defense.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (31701800, 31972338, 31772176, 31672038 and 31621064), the National Key Research and Development Program of China (2019YFD0300100 and 2017YFD0201900) and the Open Fund Project of State Key Laboratory for Biology of Plant Diseases and Insect Pests (SKLOF201901)

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Authors and Affiliations

  1. Department of Entomology, MOA Key Lab of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing, 100193, China

    Xinzheng Huang & Wangpeng Shi

  2. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China

    Xinzheng Huang, Junfeng Kou, Weixia Jing, Danfeng Liu, Somayyeh Ghasemzadeh, Peiyao Sun & Yongjun Zhang

  3. Institute of Plant Protection, Cangzhou Academy of Agriculture and Forestry Sciences, Cangzhou, 061001, China

    Junfeng Kou

  4. College of Agriculture, Key Laboratory of Oasis Agricultural Pest Management and Plant Protection Resources Utilization, Xinjiang Uygur Autonomous Region, Shihezi University, Shihezi, 832003, China

    Xiaoqiang Han

  5. Laboratory of Ecology and Evolutionary Biology, Yunnan University, Kunming, 650091, China

    Danfeng Liu

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  1. Xinzheng Huang
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  2. Junfeng Kou
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Correspondence to Yongjun Zhang.

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Huang, X., Kou, J., Jing, W. et al. Transcriptomic and metabolomic reprogramming in cotton after Apolygus lucorum feeding implicated in enhancing recruitment of the parasitoid Peristenus spretus. J Pest Sci 95, 249–262 (2022). https://doi.org/10.1007/s10340-021-01369-0

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