Matrix-assisted laser desorption ionization–time of flight mass spectrometry reveals Enterococcus and Enterobacter spp. in major insect species involved in food security with resistance to common antibiotics
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Stored-product insects can transfer a wide range of serious pathogens involved in human health. The close contact of these insects with the food production chain makes these species extremely dangerous as carriers of severe infections. In addition, pathogenic bacteria, such as members of Enterococcus, are often resistant to antibiotics commonly used for human therapy. Herein we identified, using Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS), Enterococcus and coliform species isolated from 17 strains of different insect species associated with durable stored products. The antibiotic susceptibility of the isolated strains was also evaluated. MALDI-TOF MS revealed mainly the presence of Enterococcus (E. faecium, E. phoeniculicola and E. casseliflavus) and Enterobacter (Eb. cloacae and Eb. asburiae). E. casseliflavus was resistant to all antibiotics tested, while E. faecium and E. phoeniculicola were resistant to sulphonamides. Among E. faecium isolates, approx. 20% were found to be resistant to tetracycline, while Eb. cloacae and Eb. asburiae showed resistance to erythromycin. The current series of data clearly indicates that certain bacteria of the genera Enterococcus and Enterobacter are very common in stored-product insects, and, under certain circumstances, may seriously endanger public health, through potential introduction of antibiotic resistance.
KeywordsStored-product insects Amylaceous commodities Enterococcus Enterobacter MALDI-TOF-MS Antibiotic resistance
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Conflict of interest
The authors declare that they have no conflict of interest.
This article does not contain any studies with animals performed by any of the authors.
- Gorham JR (1977) Training manual for analytical entomology in the food industry. FDA technical bulletin no, FDA US Department of Health, WashingtonGoogle Scholar
- Healthcare Infection Control Practices Advisory Committee (HICPAC) (2016) Antibiotic stewardship statement for antibiotic guidelines—The Recommendations of the Healthcare Infection Control Practices Advisory CommitteeGoogle Scholar
- Hubert J, Stejskal V, Athanassiou CG, Throne JE (2018) Health hazards associated with arthropod infestation of stored products. Annu Rev Entomol 63:553–573. https://doi.org/10.1146/annurev-ento-020117-043218 CrossRefGoogle Scholar
- Lebreton F, Willems RJL, Gilmore MS (2014) Enterococcus diversity, origins in nature, and gut colonization. In: Gilmore MS, Clewell DB, Ike Y, Shankar N (eds) Enterococci: from commensals to leading causes of drug resistant infection. Massachusetts Eye and Ear Infirmary, BostonGoogle Scholar
- Omaye ST, Wirtz RA, Fruin JT (1981) Toxicity of substituted p-benzoquinones found in the secretion of tenebrionid flour beetles. Proc West Pharmacol Soc 24:169–171Google Scholar
- Tanada Y, Kaya HK (1993) Insect Pathology. Academic Press, San DiegoGoogle Scholar
- Zurek L, Gorhamm JR (2010) Insects as vectors of foodborne pathogens. In: Voeller JG (ed) Wiley handbook of science and technology for homeland security. Wiley, Hoboken, pp 1169–1683Google Scholar