Assessment of a commercial spider venom peptide against spotted-wing Drosophila and interaction with adjuvants
Chemical control of insect pests in food crops is dominated by broad-spectrum insecticides from a few classes, and there is an urgent need for alternative modes of action. We examined the efficacy of a spider venom peptide, GS-omega/kappa-Hxtx-Hv1a (hereafter, Hv1a) for control of spotted-wing Drosophila and evaluated the importance of phagostimulants and adjuvants for its efficacy. Topical and residual activity of Hv1a was low, with only 17.5% of exposed adult D. suzukii dying after 72 h. In contrast, 100% adult mortality was observed after 24 h when three adjuvants were added to Hv1a. Survival of eggs of D. suzukii oviposited into blueberries was also reduced by exposure to Hv1a combined with the same adjuvants, indicating that Hv1a activity against D. suzukii in the laboratory, but requires penetration of the insect cuticle for efficacy. In a field trial in blueberries, Hv1a gave comparable control to phosmet, and significantly reduced infestation in fruit. This biopesticide adds a new mode of action to the options available for integrated pest management of this and other insect’s pests.
KeywordsAdjuvants Blueberries Biopesticide Drosophila
The authors acknowledge the excellent assistance of Elizabeth Espeland with bioassays and maintaining cultures of Drosophila suzukii. The authors would like to thank the anonymous reviewers for their valuable comments and suggestions to improve the quality of the paper.
This study was funded in part by the USDA National Institute for Food and Agriculture through the Specialty Crop Research Initiative (award 2015-51181-24252) and the Organic Research and Extension Initiative (award 2015-51300-24154). This research was supported in part by chemical companies providing pesticides and/or research funding.
Compliance with ethical standards
Human and animal rights statement
All applicable international, national and/or institutional guidelines for the care and use of animals were followed. This article does not contain any studies with human participants performed by any of the authors. Informed consent was obtained from all individual participants included in the study.
- Environmental Protection Agency (2014) GS-omega/kappa-Hxtx-Hv1a; Exemption From the Requirement of a Tolerance. https://www.federalregister.gov/documents/2014/02/26/2014-04092/gs-omegakappa-hxtx-hv1a-exemption-from-the-requirement-of-a-tolerance. Accessed 16 Oct 2017
- Fanning PD, Grieshop MJ, Isaac R (2017) Efficacy of biopesticides on spotted wing drosophila, Drosophila suzukii Matsumura in fall red raspberries. J App Entomol 142(1–2):26–32Google Scholar
- Jiang H, Zhu Y, Chen Z (1995) Insect resistance of transformed tobacco plants with gene of the spider insecticidal peptide. Acta Bot Sin 38:95–99Google Scholar
- Mota-Sanchez, Wise (2017) Arthropod Pesticide Resistance Database. Assessed 15 Oct 2017Google Scholar
- R Core Team (2016) R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. URL https://www.R-project.org/
- Van Timmeren S, Diepenbrock LM, Bertone MA, Burrack HJ, Isaacs R (2017b) A filter method for improved monitoring of Drosophila suzukii (Diptera: Drosophilidae) larvae in fruit. J Integr Pest Manag 8:23Google Scholar
- Wise J, Whalon M (2009) A systems approach to IPM integration, ecological assessment and resistance management in tree fruit orchards. In: Ishaaya I, Rami Horowitz A (eds) Biorational control of arthropod pests: application and resistance management. Springer, Dordrecht, pp 325–345CrossRefGoogle Scholar
- Wise JC, VanWoerkom A, Isaacs R (2017) Control of spotted wing Drosophila in blueberries, 2016. ATM 42:tsx064-tsx064 https://doi.org/10.1093/amt/tsx064