Stilbenes from grapevine root: a promising natural insecticide against Leptinotarsa decemlineata

Journal of Pest Science volume 91, pages897–906(2018)Cite this article

Abstract

Stilbenes are phenolic compounds which are produced in large amounts in vine and are involved in plant defence as phytoalexins. Oligomeric forms have recently proven to be the most active compounds against a wide range of parasites such as fungi, bacteria or algae. The aim of this study was to investigate the activity of a grapevine root extract which is a stilbene oligomer pool against Leptinotarsa decemlineata, a major pest of Solanaceae crops. Analysis by UHPLC-DAD-MS of the stilbene-enriched extract obtained from grapevine root (Riparia Gloire de Montpellier rootstock) highlighted twelve stilbenes at 25% (w/w). The major stilbenes found in root extract were isolated such as the tetramers vitisin B, vitisin A and hopeaphenol; the dimers ampelopsin A and E-ε-viniferin and the monomer E-resveratrol. The insecticidal effects of this extract as well as the main compounds were investigated against L. decemlineata larvae. The extract caused chronic toxicity, inhibited larval development and, to a lesser extent, inhibited food intake. The high concentrations of vitisin A and vitisin B in grapevine root contributed to this effect as they are the most toxic compounds. Outdoor pot experiments revealed the efficacy of stilbene-enriched extract with high mortality of L. decemlineata and protection of potato plants. The extract also revealed an absence of toxicity against non-targeted organisms such as earthworms (Eisenia fetida). Thus, these results strongly suggest that grapevine roots are a promising source of bioactive stilbenes for the development of natural insecticides.

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Acknowledgements

Dr. Pavela would like to thank the Ministry of Agriculture in the Czech Republic for financial support of botanical pesticide and basic substance research. Financial support for this work was provided by Project No. QJ1610082.

Funding

The authors also wish to thank the Conseil Regional d’Aquitaine for their financial support in this research. The work was supported by the Bordeaux Metabolome Facility and MetaboHUB (ANR-11-INBS-0010 Project).

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Affiliations

  1. Faculté des Sciences Pharmaceutiques, Unité de Recherche Œnologie EA 4577, USC 1366 INRA, Equipe Molécules d’Intérêt Biologique (Gesvab) - Institut des Sciences de la Vigne et du Vin, Université de Bordeaux, CS 50008 - 210, Chemin de Leysotte, 33882, Villenave d’Ornon, France

    Julien Gabaston, Toni El Khawand, Pierre Waffo-Teguo, Alain Decendit, Tristan Richard & Jean-Michel Mérillon

  2. Crop Research Institute, Research Team – Biologically Active Substances in Crop Protection, Drnovska 507, 161 06, Prague 6 - Ruzyne, Czech Republic

    Roman Pavela

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  1. Julien Gabaston
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  2. Toni El Khawand
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  3. Pierre Waffo-Teguo
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  4. Alain Decendit
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  5. Tristan Richard
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  6. Jean-Michel Mérillon
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Contributions

RP, PWT and JMM conceived the project. JG, TK, TR, AD and RP performed the experiments. JG, JMM and RP wrote the manuscript. All authors read and approved the manuscript.

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Correspondence to Jean-Michel Mérillon.

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Communicated by M.B. Isman.

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Gabaston, J., El Khawand, T., Waffo-Teguo, P. et al. Stilbenes from grapevine root: a promising natural insecticide against Leptinotarsa decemlineata. J Pest Sci 91, 897–906 (2018). https://doi.org/10.1007/s10340-018-0956-2

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Keywords

  • Botanical insecticide
  • Solanaceae
  • Colorado potato beetle
  • Vine root extract
  • Stilbene
  • Vitisins