Journal of Pest Science

, Volume 90, Issue 3, pp 947–960 | Cite as

Efficacy of entomopathogenic fungi against adult Diaphorina citri from laboratory to field applications

  • John J. Saldarriaga Ausique
  • Celeste Paola D’AlessandroEmail author
  • Marcos Roberto Conceschi
  • Gabriel Moura Mascarin
  • Italo Delalibera Júnior
Original Paper


The Asian citrus psyllid, Diaphorina citri, is the most serious threat to citrus crops worldwide, and its management relies exclusively on frequent applications of chemical insecticides. Entomopathogenic fungi may play an important role for regulating this citrus pest. Two virulent fungal strains, Isaria fumosorosea ESALQ-1296 and Beauveria bassiana ESALQ-PL63, were selected among 17 fungal isolates under laboratory conditions and caused 77.8 and 78.4% adult mortality, respectively, while in semifield trials the adult mortality reached up to 83.5% with B. bassiana and 80.6% with I. fumosorosea. The bioefficacy of these two fungi on D. citri adults was assessed through monthly applications during 1 year in a commercial citrus grove located in Itapetininga, São Paulo, Brazil. The application of I. fumosorosea ESALQ-1296 and B. bassiana ESALQ-PL63 was as effective as the chemical insecticides in most trials. The fungal-induced mortality of D. citri adults ranged from 96.1% in December 2011 to 57.8% in October 2012. This mortality level tended to build up with the increase in the maximum relative humidity; the percentage of sporulated cadavers was positively correlated with both higher relative humidity and rainfall but negatively associated with maximum temperatures. These results support the use of I. fumosorosea or B. bassiana as an eco-friendly biopesticide for the integrated management of D. citri.


Microbial control Hypocrealean fungi Asian citrus psyllid Huanglongbing Citrus 



We thank the Foundation of Citrus Growers of São Paulo State (Fundecitrus), Koppert Sistemas Biológicos LTDA and the São Paulo State Research Foundation (FAPESP Project No. 2011/51556-3) for financial support. The first author was a recipient of a scholarship from the Coordination of Improvement of Higher Education Personnel (CAPES PEC/PG—Brazil 2010–2014).


This study was funded by the Foundation of Citrus Growers of São Paulo State (Fundecitrus), Koppert Sistemas Biológicos LTDA and the São Paulo State Research Foundation (FAPESP Project No. 2011/51556-3).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

10340_2017_846_MOESM1_ESM.docx (274 kb)
Supplementary material 1 (DOCX 274 kb)


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Copyright information

© Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  • John J. Saldarriaga Ausique
    • 1
  • Celeste Paola D’Alessandro
    • 2
    Email author
  • Marcos Roberto Conceschi
    • 2
  • Gabriel Moura Mascarin
    • 3
  • Italo Delalibera Júnior
    • 2
  1. 1.Plant Protection Laboratory, Institute of Agricultural ScienceFederal Rural University of AmazonBelémBrazil
  2. 2.Department of Entomology and Acarology, “Luiz de Queiroz” College of Agriculture (ESALQ)University of São Paulo (USP)PiracicabaBrazil
  3. 3.EMBRAPA Rice and BeansSt. Antônio de GoiásBrazil

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