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Fungal endophytes as promising tools for the management of bean stem maggot Ophiomyia phaseoli on beans Phaseolus vulgaris

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Abstract

Common bean, Phaseolus vulgaris, is an important food and cash crop in Africa. Its production is seriously affected by the bean stem maggot (BSM), Ophiomyia spp., which attacks seedlings. We evaluated the ability of eleven fungal isolates to colonize bean plants and the effects of inoculation on BSM feeding and oviposition, pupation, and adult emergence. All fungal isolates were able to colonize different bean plant parts (root, stem, and leaves), except isolates of Metarhizium anisopliae and Beauveria bassiana isolate ICIPE 273. Colonization was generally higher on the roots than on the stem and leaves and varied significantly between the fungal isolates. BSM feeding and oviposition were significantly reduced in all the fungus-inoculated bean plants which in turn affected pupation and adult emergence as compared to the control. Metarhizium anisopliae ICIPE 20 outperformed the other isolates in interfering with BSM lifecycle. Although M. anisopliae ICIPE 78 recorded a high number of punctures similar to the control, a significant reduction in the number of pupae and adult emergence was observed, suggesting possible BSM growth inhibition. This study clearly demonstrates that fungal endophytes can be considered as promising tools for the management of BSM in East Africa.

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Acknowledgments

This study was funded icipe’s Innovative Seed Research Grant “Bean Stem Maggot”. The authors are very grateful to Mrs Gloria Muthoni for technical assistance. We acknowledge the statistical analysis assistance from Dr. Daisy Salifu and taxonomical assistance from Dr. Robert Copeland of icipe and Dr. Fathiya Khamis for molecular analysis.

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Authors and Affiliations

  1. International Centre of Insect Physiology and Ecology (icipe), P.O. Box 30772-00100, Nairobi, Kenya

    Beritah Mutune, Sunday Ekesi, Saliou Niassy & Nguya K. Maniania

  2. Jomo Kenyatta University of Agriculture and Technology, P.O. Box 62000-00200, Nairobi, Kenya

    Beritah Mutune, Viviene Matiru & Christine Bii

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  1. Beritah Mutune
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  2. Sunday Ekesi
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  3. Saliou Niassy
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  4. Viviene Matiru
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  6. Nguya K. Maniania
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Correspondence to Nguya K. Maniania.

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Communicated by S. T. Jaronski.

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Mutune, B., Ekesi, S., Niassy, S. et al. Fungal endophytes as promising tools for the management of bean stem maggot Ophiomyia phaseoli on beans Phaseolus vulgaris . J Pest Sci 89, 993–1001 (2016). https://doi.org/10.1007/s10340-015-0725-4

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  • DOI: https://doi.org/10.1007/s10340-015-0725-4

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