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Journal of Pest Science

, Volume 88, Issue 1, pp 1–7 | Cite as

A pest alien invasion in progress: potential pathways of origin of the brown marmorated stink bug Halyomorpha halys populations in Italy

  • Michele Cesari
  • Lara Maistrello
  • Francesco Ganzerli
  • Paride Dioli
  • Lorena Rebecchi
  • Roberto Guidetti
Rapid Communication

Abstract

The brown marmorated stink bug, Halyomorpha halys, is an agricultural and household pest native of far East. In the last years, it has spread to and established in countries outside its area of origin, most notably in North America (United States and Canada), causing severe economic losses in agricultural crops. Recently, H. halys has been found in Europe (Switzerland, Germany, France, Hungary, and Greece) and since September 2012, it has also been found in Italy. However, the modalities of introduction and spreading of this pest on the Italian territory are unknown. Tracing back the diffusion modes of the species by analyzing the genetic structure and composition of populations in their initial phase of colonization could be useful also in the view to implement better pest control strategies. The present study aimed to identify the potential pathways of entry of H. halys by detecting the genetic diversity of specimens collected from Northern Italy and Canton Ticino (Southern Switzerland). The analyses of 1,175 base pairs of mtDNA cytochrome c oxidase I and II genes (cox1 and cox2) on 42 specimens led to the identification of four combined haplotypes: one, found in Emilia Romagna region, is the same found in China and North America but never observed before in Europe. The other combined haplotypes are new but consistent in part with haplotypes previously found in Switzerland. Present data indicate that the Italian invasion may have occurred from two different pathways, both from Switzerland and from Asia and/or North America.

Keywords

Heteroptera Pentatomidae Invasive species Haplotype network Agricultural pest DNA barcoding 

Notes

Acknowledgments

This research was supported by the Grant “Innovative tools and protocols for monitoring and sustainable control of the alien stink bug Halyomorpha halys, a new phytosanitary threat, and of other harmful heteropterans for the fruit crops of the territory of Modena” (2013.065) of “Fondazione Cassa di Risparmio di Modena,” Modena, Italy. We want to thank all the people who provided us the H. halys specimens.

Supplementary material

10340_2014_634_MOESM1_ESM.docx (15 kb)
Table S1 Sequences retrieved from GenBank and BOLD Systems for appropriate comparisons (DOCX 16 kb)
10340_2014_634_MOESM2_ESM.pdf (16 kb)
Table S2 Uncorrected p-distance (below the diagonal) and Kimura 2-parameters (above the diagonal) distances computed among all specimens. The analysis was carried out on 657 bp of cox1 gene (PDF 17 kb)
10340_2014_634_MOESM3_ESM.docx (44 kb)
Table S3 Uncorrected p-distance (below the diagonal) and Kimura 2-parameters (above the diagonal) distances computed among all specimens. The analysis was carried out on 518 bp of cox2 gene (DOCX 44 kb)
10340_2014_634_MOESM4_ESM.docx (19 kb)
Table S4 Uncorrected p-distance (below the diagonal) and Kimura 2-parameters (above the diagonal) distances computed among all specimens. The analysis was carried out on 1,175 bp of both cox1 and cox2 genes (DOCX 20 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Michele Cesari
    • 1
  • Lara Maistrello
    • 1
  • Francesco Ganzerli
    • 1
  • Paride Dioli
    • 2
  • Lorena Rebecchi
    • 1
  • Roberto Guidetti
    • 1
  1. 1.Department of Life SciencesUniversity of Modena and Reggio EmiliaModenaItaly
  2. 2.Museo Civico di Storia Naturale di MilanoMilanItaly

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