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Efficacy of entomopathogenic Steinernema and Heterorhabditis nematodes against Holotrichia oblita

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Abstract

The white grub Holotrichia oblita Faldermann (Coleoptera: Scarabaeidae) causes serious damage in peanut fields in China. The development of an environmentally friendly control method for this pest is urgently needed. The efficacy of entomopathogenic nematodes against this pest in the laboratory and peanut fields was evaluated in this study. Both Steinernema longicaudum Shen and Wang and Heterorhabditis bacteriophora Poinar (Rhabditida: Steinernematidae and Heterorhabditidae) had promising control efficacy against H. oblita larvae in the laboratory. S. longicaudum X-7 and H. bacteriophora H06 at 1.0 × 104 and 5.0 × 103 IJs per plant caused a similar percentage reduction of the grub larvae and percentage of the injured legumes to chlorpyrifos. The peanut yields from the nematode-treated plots at 5.0 × 103 IJs per plant were at least 55 and 15 % higher than those from the water control and the chlorpyrifos-treated plots. No significant differences were found in the percentage reductions of the grub larvae at different larval stage applications. The peanut yields in plots treated by S. longicaudum X-7 and H. bacteriophora H06 were also not significantly different. The cost-benefit analysis showed that S. longicaudum X-7 and H. bacteriophora H06 are promising agents for H. oblita larvae control in peanut fields. Our findings indicate that entomopathogenic nematodes have good potential for safe management of H. oblita in peanut production.

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References

  • Abbott WS (1925) A method of computing the effectiveness of an insecticide. J Econ Entomol 18:265–267

    Article  CAS  Google Scholar 

  • Bedding RA (1981) Low cost in vitro mass production of Neoaplectana and Heterorhabditis species (Nematoda) for field control of insect pests. Nematologica 27(1):109–114

    Article  Google Scholar 

  • Choo HY, Kaya HK, Huh J, Lee DW, Kim HH, Lee SM, Choo YM (2002) Entomopathogenic nematodes (Steinernema spp. and Heterorhabditis bacteriophora) and a fungus Beauveria brongniartii for biological control of the white grubs, Ectinohoplia rufipes and Exomala orientalis, in Korean golf courses. Biocontrol 47:177–192

    Article  Google Scholar 

  • Du X, Liu Q, Zhang L, Liang L, Xie N, Zhang S (2009) Application technology of Steinernema longicaudum BPS strain in peanut fields for chafer grub control. Agrochemicals 48(5):379–388

    Google Scholar 

  • Ehlers R-U (2001) Mass production of entomopathogenic nematodes for plant protection. Appl Microbiol Biotechnol 56:623–633

    Article  CAS  PubMed  Google Scholar 

  • Forschler BT, Gardner WA (1991) Field efficacy and persistence of entomogenous nematodes in the management of white grubs (Coleoptera: Scarabaeidae). J Econ Entomol 84:1454–1459

    Article  Google Scholar 

  • Gaugler R (1998) Ecological consideration in the biological control using entomopathogenic nematodes. Agr Ecosyst Environ 24:351

    Article  Google Scholar 

  • Georgis R, Gaugler R (1991) Predictability in biological control using entomopathogenic nematodes. J Econ Entomol 84(3):713–720

    Article  Google Scholar 

  • Georgis R, Koppenhöfer AM, Lacey LA, Be´lair G, Duncan LW, Grewal PS, Samish M, Tan L, Torr P, van Tol RWHM (2006) Successes and failures in the use of parasitic nematodes for pest control. Biol Control 38:103–123

    Article  Google Scholar 

  • Glazer I, Gol’berg A (1989) Laboratory evaluation of steinernematid and heterorhabditid nematodes for control of the beetle Maladera matrida Argaman (Coleoptera: Scarabaeidae). Phytoparasitica 17:3–11

    Article  Google Scholar 

  • Glazer I, Gol’berg A (1993) Field efficacy of entomopathogenic nematodes against the beetle Maladera matrida (Coleoptera: Scarabaeidae). Bioc Sci Technol 3:367–376

    Article  Google Scholar 

  • Grewal PS (2002) Formulation and application technology. In: Gaugler R (ed) Entomopathogenic nematology. CABI Publishing, Wallingford, pp 265–288

    Chapter  Google Scholar 

  • Grewal PS, Power KT, Grewal SK, Suggars A, Haupricht S (2004) Enhanced consistency in biological control of white grubs (Coleoptera: Scarabaeidae) with new strains of entomopathogenic nematodes. Biol Control 30:73–82

    Article  Google Scholar 

  • Grewal PS, Koppenhöfer AM, Choo HY (2005) Lawn, turfgrass and pasture applications. In: Grewal PS, Ehlers R-U, Shapiro-Ilan DI (eds) Nematodes as biocontrol agents. CABI Publishing, Wallingford, pp 115–146

    Chapter  Google Scholar 

  • Guo WX, Yan X, Zhao GY, Han RC (2013) Efficacy of entomopathogenic Steinernema and Heterorhabditis nematodes against white grubs (Coleoptera: Scarabaeidae) in peanut fields. J Econ Entomol 106(3):1112–1117

    Article  CAS  PubMed  Google Scholar 

  • Han RC (1996) The effects of inoculum size on yield of Steinernema carpocapsae and Heterorhabditis bacteriophora in liquid culture. Nematologica 42:546–553

    Article  Google Scholar 

  • Khatri-Chhetri HB, Timsina GP, Manandhar HK, Moens M (2011) Potential of Nepalese entomopathogenic nematodes as biocontrol agents against Holotrichia longipennis Blanch. (Coleoptera: Scarabaeidae). J Pest Sci 84:457–469

    Article  Google Scholar 

  • Koppenhöfer AM, Fuzy EM (2003a) Steinernema scarabaei for the control of white grubs. Biol Control 28:47–59

    Article  Google Scholar 

  • Koppenhöfer AM, Fuzy EM (2003b) Effects of turfgrass endophytes (Clavicipitaceae: Ascomycetes) on white grub (Coleoptera: Scarabaeidae) control by the entomopathogenic nematode Heterorhabditis bacteriophora (Rhabditida: Heterorhabditidae). Environ Entomo 32:392–396

    Article  Google Scholar 

  • Koppenhöfer AM, Fuzy EM (2004) Effect of white grub developmental stage on susceptibility to entomopathogenic nematodes. J Econ Entomol 97:1842–1849

    Article  PubMed  Google Scholar 

  • Koppenhöfer AM, Fuzy EM (2008) Early timing and new combinations to increase the efficacy of neonicotinoid–entomopathogenic nematode (Rhabditida: Heterorhabditidae) combinations against white grubs (Coleoptera: Scarabaeidae). Pest Manag Sci 64:725–735

    Article  PubMed  Google Scholar 

  • Koppenhöfer AM, Brown IM, Gaugler R, Grewal PS, Kaya HK, Klein MG (2000a) Synergism of entomopathogenic nematodes and imidacloprid against white grubs: greenhouse and field evaluation. Biol Control 19:245–252

    Article  Google Scholar 

  • Koppenhöfer AM, Brown IM, Kaya HK, Gaugler R (2000b) Synergism of entomopathogenic nematodes and imidacloprid against white grubs: greenhouse and field evaluation. Biol Control 19:245–251

    Article  Google Scholar 

  • Koppenhöfer AM, Cowles RS, Cowles EA, Fuzy EM, Baumgartner L (2002) Comparison of neonicotinoid insecticides as synergists for entomopathogenic nematodes. Biol Control 24:90–97

    Article  Google Scholar 

  • Lee W, Choo HY, Kaya HK, Lee SM, Smitley DR, Shin HK, Park CG (2002) Laboratory and Weld evaluation of Korean entomopathogenic nematode isolates against the oriental beetle Exomala orientalis (Coleoptera: Scarabaeidae). J Econ Entomol 95:918–926

    Article  PubMed  Google Scholar 

  • Li S, Ding W, Han F (1993) Application of entomophagous nematode Taishan No. 1 against peanut grubs. Acta Phytophylacica Sin 26(1):55–60

    Google Scholar 

  • Li S, Liu A, Wu Y, Luo J, Yin H (2003) Species, distribution, damage and integrated management methods of dominant white grubs in Henan province. J Henan Agric Sci 4:16–18

    Google Scholar 

  • Li J, Sun C, Kang Y, Ma J (2007) Control effect of entomopathogenic nematodes to peanuts grubs. Agrochemica 46(1):62–63

    Google Scholar 

  • Li YF, Gao ZL, Dang ZH, Pan WL, Tao LM (2008) Comparison on the toxicities of 18 insecticides to Holotrichia oblita Faldermann and Anomala corpulenta Motschulsky. Chin Agric Sci Bull 24(3):296–299

    Google Scholar 

  • Liu Q, Li J, Xu X, Sun C, Kang Y, Zhou H, Hu D, Ma J, Li S (2007) The preliminary study on grub control with Rhabditis (Oscheius) spp in peanut fields. Acta Agric Boreal Sin 22:250–253

    Google Scholar 

  • Liu Q, Du X, Zhang L, Zhang S, Xie N, Liang L (2009a) Effectiveness of Steinernema longicaudum BPS for chafer grub control in peanut plot. Plant Prot 35(6):150–153

    Google Scholar 

  • Liu S, Li K, Liu C, Wang Q, Yin J, Cao Y (2009b) Identification of a strain of Heterorhabditis (Nematoda: Heterorhabditidae) from Hebei and its virulence to white grubs. Acta Entomo Sin 52(9):959–966

    CAS  Google Scholar 

  • Ma J, Chen SL, Moens M, Han RC, De Clercq P (2013) Efficacy of entomopathogenic nematodes (Rhabditida: Steinernematidae and Heterorhabditidae) against the chive gnat, Bradysia odoriphaga. J Pest Sci 86:551–561

    Article  Google Scholar 

  • Mukuka J, Strauch O, Hoppe C, Ehlers R-U (2010) Fitness of heat and desiccation tolerant hybrid strains of Heterorhabditis bacteriophora (Rhabditidomorpha: Heterorhabditidae). J Pest Sci 83:281–287

    Article  Google Scholar 

  • Shapiro-Ilan DI, Han RC, Dolinksi C (2012) Entomopathogenic nematode production and application technology. J Nematol 44(2):206–217

    PubMed Central  PubMed  Google Scholar 

  • Susurluk A, Ehlers R-U (2008) Field persistence of the entomopathogenic nematode Heterorhabditis bacteriophora in different crops. Biocontrol 53:627–641

    Article  Google Scholar 

  • Susurluk IA, Kumral NA, Bilgili U, Açıkgöz E (2011) Control of a new turf pest, Dorcadion pseudopreissi (Coleoptera: Cerambycidae), with the entomopathogenic nematode Heterorhabditis bacteriophora. J Pest Sci 84:321–326

    Article  Google Scholar 

  • Toepfer S, Kurtz B, Kuhlmann U (2010) Influence of soil on the efficacy of entomopathogenic nematodes in reducing Diabrotica virgifera virgifera in maize. J Pest Sci 83:257–264

    Article  Google Scholar 

  • Wang XL (2004) The regularity and reason of outbreak and control resources against white grubs in peanut field. J Anhui Agric Sci 32(2):287–303

    Google Scholar 

  • Wang BL, Wang HL (2006) The outbreak and integrated management of white grubs in peanut fields. J Henan Agric Sci 7:66–68

    Google Scholar 

  • Yan X, Liu XJ, Han RC, Chen SR, De Clercq P, Moens M (2010) Osmotic induction of anhydrobiosis in entomopathogenic nematodes of the genera Heterorhabditis and Steinernema. Biol Control 53:325–330

    Article  Google Scholar 

  • Yan X, Moens M, Han RC, Chen SL, De Clercq P (2012) Effects of selected insecticides on osmotically treated entomopathogenic nematodes. J Plant Dis Protect 119(4):152–158

    CAS  Google Scholar 

  • Yan X, Han RC, Moens M, Chen SL, De Clercq P (2013) Field evaluation of entomopathogenic nematodes for biological control of striped flea beetle, Phyllotreta striolata (Coleoptera: Chrysomelidae). Biocontrol 58:247–256

    Article  Google Scholar 

  • Zhang YL, Yuan YH, Yuan GH, Guo XR, Luo MH (2006) A study on the attraction of Holotrichia oblita (Faldermann) to castor leaves. J Henan Agric Univ 40(1):53–57

    Google Scholar 

  • Zhang J, Zhao Z, Liang J, Zheng X, Wu W, Lin J (2009) Residue determination of chlorpyrifos in apple fruit, leaf and orchard soil. Chin Agric Sci Bull 25(01):185–189

    Google Scholar 

Download references

Acknowledgments

This study was supported by the National High-Technology Research and Development Project (863 Project; 2011AA10A201), Nonprofit Sector Project (201003025), National Natural Science Foundation of China (31101494), Guangdong Provincial Science & Technology Project (2012B050700008), Guangzhou Science & Technology Project (2013J2200090) and funding from Guangdong Academy of Sciences for Excellent Young Scientists (rcjj201201).

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. South China Botanical Garden, Chinese Academy of Sciences, 723 Xinke Road, Guangzhou, 510650, China

    Wenxiu Guo

  2. Guangdong Key Laboratory of Integrated Pest Management in Agriculture & Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Guangdong Entomological Institute, 105 Xingang Road West, Guangzhou, 510260, China

    Wenxiu Guo, Xun Yan, Guoyu Zhao, Jinghua Chen & Richou Han

  3. University of Chinese Academy of Sciences, 19A Yuquan Road, Beijing, 100049, China

    Wenxiu Guo

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  1. Wenxiu Guo
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  2. Xun Yan
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  3. Guoyu Zhao
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  4. Jinghua Chen
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  5. Richou Han
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Corresponding author

Correspondence to Richou Han.

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Communicated by R.-U. Ehlers.

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Guo, W., Yan, X., Zhao, G. et al. Efficacy of entomopathogenic Steinernema and Heterorhabditis nematodes against Holotrichia oblita . J Pest Sci 88, 359–368 (2015). https://doi.org/10.1007/s10340-014-0626-y

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  • DOI: https://doi.org/10.1007/s10340-014-0626-y

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