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Rapid discrimination of two cryptic species within Brontispa longissima (Gestro) (Coleoptera: Chrysomelidae) by PCR–RFLP

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Abstract

Brontispa longissima (Coleoptera: Chrysomelidae) is a serious invasive pest of coconut palm (Cocos nucifera) supposedly originating from Indonesia and New Guinea. It has recently invaded Southeast and East Asia, where it has caused serious damage to coconut plants. Brontispa longissima as currently defined contains two cryptic species: we herein referred to one as the “Asian clade”, which is distributed over a wide area, including Asia and the Pacific region; and we referred to the other one as the “Pacific clade”, which is found in a limited area in the Pacific region. We developed a PCR–RFLP method for differentiating the two clades. Digestion of the PCR product of a 1,014-bp region within the mitochondrial cytochrome oxidase I gene (COI) with BslI, HpyCH4III, or NlaIV resulted in clade-specific patterns as estimated by the sequence data. We applied the method to specimens newly obtained from various locations to investigate the geographical distribution of B. longissima. Although B. longissima collected from Samoa in April 2003 had been placed in the Pacific clade, specimens collected from the same island in April 2010 were placed in the Asian clade, suggesting that the predominant clade may have been changing from the former to the latter. On Timor, specimens included both clades in apparently segregated habitats.

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Acknowledgments

We are grateful to Wilco Liebregts, Ketut Sumiartha, Wayan Susila, Matias Tavares, Donny Tungga, and Sri Wahyuni for their help in collecting specimens. We also thank Kayoko Furukawa for her technical assistance. This research was supported by a JIRCAS project (Development of Biological Control against Invasive Insect Pests on Coconut Trees) and a Grant-in Aid for Scientific Research (A) (no. 21255008).

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  1. Shun-ichiro Takano

    Present address: Yokohama Plant Protection Station, 1-16-10 Shin-yamashita, Naka-ku, Yokohama, 231-0801, Japan

Authors and Affiliations

  1. Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, 744 Motooka, Fukuoka, 819-0395, Japan

    Shun-ichiro Takano

  2. Biodiversity Division, National Institute for Agro-Environmental Sciences, 3-1-3 Kannondai, Tsukuba, Ibaraki, 305-8604, Japan

    Atsushi Mochizuki

  3. Faculty of Agriculture, Kyushu University, 744 Motooka, Fukuoka, 819-0395, Japan

    Keiji Takasu

  4. National Agricultural Research Center for the Hokkaido Region, Sapporo, Hokkaido, 062-8555, Japan

    Kazuhiko Konishi

  5. Indonesian Coconut and Palmae Research Institute, Jl. Bethesda II, Mapanget Kotak Pos 1004, Manado, 65001, North Sulawesi, Indonesia

    Jelfina C. Alouw & Donata S. Pandin

  6. Japan International Research Center for Agricultural Sciences, 1-1 Ohwashi, Tsukuba, Ibaraki, 305-8686, Japan

    Satoshi Nakamura

Authors
  1. Shun-ichiro Takano
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  2. Atsushi Mochizuki
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  3. Keiji Takasu
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  4. Kazuhiko Konishi
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  5. Jelfina C. Alouw
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  6. Donata S. Pandin
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  7. Satoshi Nakamura
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Corresponding author

Correspondence to Shun-ichiro Takano.

Additional information

Communicated by A. Juen.

Atsushi Mochizuki contributed equally to this study.

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Takano, Si., Mochizuki, A., Takasu, K. et al. Rapid discrimination of two cryptic species within Brontispa longissima (Gestro) (Coleoptera: Chrysomelidae) by PCR–RFLP. J Pest Sci 86, 151–155 (2013). https://doi.org/10.1007/s10340-012-0474-6

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  • DOI: https://doi.org/10.1007/s10340-012-0474-6

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