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Acaricidal, repellent and oviposition deterrent activities of Datura stramonium L. against adult Tetranychus urticae (Koch)

Abstract

The ethanol extracts obtained from both leaf and seed in the Thorn apple (Datura stramonium L.) (Solanaceae) were investigated for acaricidal, repellent and oviposition deterrent properties against adult two-spotted spider mites (Tetranychus urticae Koch) (Acari: Tetranychidae) under laboratory conditions. Leaf and seed extracts, which were applied in 167,250 and 145,750 mg/l concentrations, respectively (using a Petri leaf disc-spray tower method), caused 98 and 25% mortality among spider mite adults after 48 h. The simple logistic regression analysis showed that the independent variable, an increase in the dose of leaf extract was associated with a significant increase in the death rate of T. urticae females, but an increase in the dosage of seed extracts did not have a significant effect. Using probit analysis and estimating the parameters with a confidence limit of 95%, we determined the LC50 values of leaf extract to be 70,593 mg/l. According to Pearson’s χ2 test, mites showed the strongest run off to bean leaf surfaces sprayed with both leaf and seed extracts (in sub-lethal doses: <7,500 mg/l and <25,000 mg/l, respectively) and moved towards surfaces that had not been sprayed with extracts. Furthermore, repeated measures ANOVA indicated a significant difference between the number of eggs laid on unsprayed bean leaves compared to bean leaves that were sprayed with leaf and seed extracts at sub-lethal doses, 2,500 and 25,000 mg/l concentrations, respectively. These results suggest that D. stramonium extracts could be used to manage the two-spotted spider mite.

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Acknowledgements

We thank the Scientific and Technical Research Council of Turkey (Projects Grant No. 108 O 363) for financial assistance and American Journal Experts for a critical reading of the manuscript.

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Correspondence to Nabi Alper Kumral.

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Communicated by T. Haye.

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Kumral, N.A., Çobanoğlu, S. & Yalcin, C. Acaricidal, repellent and oviposition deterrent activities of Datura stramonium L. against adult Tetranychus urticae (Koch). J Pest Sci 83, 173–180 (2010). https://doi.org/10.1007/s10340-009-0284-7

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  • DOI: https://doi.org/10.1007/s10340-009-0284-7

Keywords

  • Acaricidal
  • Oviposition deterrent
  • Repellent
  • Spider mite
  • Thorn apple