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Genetic variation and mtCOI phylogeny for Bemisia tabaci (Hemiptera, Aleyrodidae) indicate that the ‘B’ biotype predominates in Iran

Journal of Pest Science volume 81, pages 199–206 (2008)Cite this article

Abstract

Despite a large number of investigations on the molecular genetics and population structure of the whitefly Bemisia tabaci (Gennadius) complex, no such study had been conducted in Iran. The genetic variation of B. tabaci was examined using polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) for 18 field collections from cucumber, eggplant, and tomato in four provinces of Iran. PCR amplification and restriction digestion with two enzymes detected 388 RFLP fragments, of which 16 fragments showed polymorphisms. Cluster analysis of these data placed all B. tabaci individuals within a single group, and there was no evidence for between- or within-population genetic variation. Phylogenetic (Clustal W) analysis of 42 B. tabaci mtCOI sequences (n = 21 field collections) from Iran, and a comparison with well-studied haplotype or biotype reference sequences available in public sequence databases, revealed that the Iranian B. tabaci populations were most closely related to the B biotype at 0–1.2% nucleotide identity. The B biotype is a well-known member of a sister clade from the Middle East–North African region of the world, owing to its nearly worldwide distribution and invasive characteristics. This report indicates that a single major haplotype of B biotype is prevalent in Iran and that its closest relative is the B biotype. Also, given the extent of known variation in the Middle East and African continent, data indicate somewhat surprisingly that the B. tabaci collections sampled in Iran had limited genetic variation and population substructure. Knowledge that the B biotype of B. tabaci predominates in Iran is important for designing effective pest management strategies given that biotypes of B. tabaci are known to differ greatly with respect to insecticide resistance, host range, virus–vector interactions, and other key biological characteristics.

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References

  • Abdullahi I, Winter S, Atiri GI, Thottappilly G (2003) Molecular characterization of whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae) populations infesting cassava. Bull Entomol Res 93(2):97–106

    Article  PubMed  CAS  Google Scholar 

  • Abdullahi I, Atiri GI, Thottappily G, Winter S (2004) Discrimination of cassava-associated Bemisia tabaci in Africa from polyphagous populations, by PCR-RFLP of the internal transcribed spacer regions of ribosomal DNA. Appl Entoml 128(2):8

    Google Scholar 

  • Avise JC (1994) Molecular markers, natural history and evolution. Chapman and Hall, New York, p 511

    Google Scholar 

  • Basu AN (1995) Bemisia tabaci (Gennadius): Crop pest and principal whitefly vector of plant viruses. West View Press, New Delhi, p 183

    Google Scholar 

  • Bananej K, Ahoonmanesh A, Kheyr-Pour A (2002) Host range of an iranian isolate of watermelon chlorotic stunt virus as determined by whitefly-mediated inoculation and agroinfection, and its geographical distribution. J Phytopath 150(8–9):423–430

    Article  Google Scholar 

  • Bedford ID, Markham PG, Brown JK, Rosell RC (1994) Geminivirus transmission and biological characterization of white-fly (Bemisia tabaci) biotypes from different world regions. Ann Appl Biol 125:311–325

    Article  Google Scholar 

  • Berry S, Rey MEC, Rogan D, Fondong VN, Fauquet CM, Brown JK (2004) Molecular evidence for distinct Bemisia tabaci geographically genotypes from cassava in Africa. Ann Entomol Soc Am 97:852–859

    Article  CAS  Google Scholar 

  • Brown JK (2000) Molecular markers for the identification and global tracking of whitefly vector-begomovirus complexes. Virus Res 71:233–260

    Article  PubMed  CAS  Google Scholar 

  • Brown JK (2007) The Bemisia tabaci complex: genetic and phenotypic variability drives begomovirus spread and virus diversification. Plant Dis. Feature Article Jan 07; http://www.apsnet.org/online/feature/btabaci/

  • Brown JK, Bird J (1992) Whitefly-transmitted geminiviruses and associated disorders in the Americas and the Caribbean Basin. Plant Dis 76:220–225

    Google Scholar 

  • Brown JK, Idris AM (2005) Genetic differentiation of the whitefly Bemisia tabaci (Genn.) mitochondria COI and geographic congruence with the coat protein of the plant virus genus: Begomovirus. Ann Entomol Soc Am 98:827–837

    Article  Google Scholar 

  • Brown JK, Coats S, Bedford ID, Markham PG, Bird J, Frohlich DR (1995) Characterization and distribution of esterase electromorphs in the whitefly, Bemisia tabaci (Genn.) (Homoptera: Aleyrodidae). Biochem Genet 33:205–214

    Article  PubMed  CAS  Google Scholar 

  • Brown JK, Perring TM, Cooper AD, Bedford ID, Markham PG (2000) Genetic analysis of Bemisia (Homoptera: Aleyrodidae) populations by isoelectric focusing electrophoresis. Biochem Genet 38:13–25

    Article  PubMed  CAS  Google Scholar 

  • Burban C, Fishpool DC, Fargette D, Thouvenel JC (1992) Host-associated biotypes within West African populations of the whitefly Bemisia tabaci (Genn.) (Hom., Aleyrodidae). J Appl Entomol 113:416–423

    Article  Google Scholar 

  • Byrne DN, Bellows TS (1991) Whitefly biology. Ann Rev Entomol 36:431–457

    Article  Google Scholar 

  • Byrne FJ, Cahill M, Denholm I, Devonshire AL (1995) Biochemical identification of interbreeding between B-type and non-B-type strains of the tobacco whitefly Bemisia tabaci. Biochem Genet 33:13–23

    Article  PubMed  CAS  Google Scholar 

  • Cervera MT, Cabezas JA, Simon B, Martinez-Zapater JM, Beitia F, Cenis JL (2000) Genetic relationships among biotypes of Bemisia tabaci (Hemiptera: Aleyrodidae) based on AFLP analysis. Bull Entomol Res 90:391–396

    Article  PubMed  CAS  Google Scholar 

  • Carr SM, Wilson AC (1987) Evolutionary inferences from restriction maps of mitochondrial DNA from nine taxa of Xenopus frogs. Evol 41:176–188

    Article  CAS  Google Scholar 

  • Costa HS, Brown JK (1991) Variation in biological characteristics and in esterase patterns among populations of Bemisia tabaci (Genn.) and the association of one population with silverleaf symptom development. Ent Exp Applic 61:211–219

    Article  Google Scholar 

  • Costa HS, Brown JK, Sivasupramaniam S, Bird J (1993) Regional distribution, insecticide resistance, and reciprocal crosses between the 'A' and 'B' biotypes of Bemisia tabaci. Insect Sci Applic 14:127–138

    Google Scholar 

  • De Barro PJ, Driver F (1997) Use of RAPD PCR to distinguish the B biotype from other biotypes of B. tabaci (Gennadius) (Hemiptera: Aleyrodidae). Aust J Entomol 36:149–152

    Article  Google Scholar 

  • De Barro PJ, Driver F, Trueman JWH, Curran J (2000) Phylogenetic relationships of world populations of Bemisia tabaci (Gennadius) using ribosomal ITS1. Mol Phylo Evol 16(1):29–36

    Article  CAS  Google Scholar 

  • De Barro PJ, Trueman JWH, Frohlich DR (2005) Bemisia argentifolii is a race of B. tabaci (Hemiptera: Aleyrodidae): the molecular genetic differentiation of B. tabaci populations around the world. Bull Entomol Res 95:193–203

    Article  PubMed  Google Scholar 

  • Ewing BL, Hillier MC, Wendl , Green P (1998) Base-calling of automated sequencer traces using phred. I. Accuracy assessment. Gen Res 8:175–85

    CAS  Google Scholar 

  • Frohlich DR, Torres-Jerez I, Bedford ID, Markham PG, Brown JK (1999) A phylogeographical analysis of the Bemisia tabaci species complex based on mitochondrial DNA markers. Mol Entomol 8:1683–1691

    Google Scholar 

  • Gawel NJ, Bartlett AC (1993) Characterisation of differences between whiteflies using RAPD-PCR. Insect Mol Biol 2:33–38

    Article  PubMed  CAS  Google Scholar 

  • Gennadius P (1889) Disease of tobacco plantations in the Trikonia.The aleyrodid of tobacco. Ellenike Georgia 5:1–3

    Google Scholar 

  • Gill RJ (1990) The morphology of whiteflies. In: Gerling P (ed) Whiteflies: their bionomics, pest status and management. Intercept Ltd, Andover, p 1346

    Google Scholar 

  • Gocmen HS, Devran ZB (2002) Determination of genetic variation in populations of Bemisia tabaci in Antalya. Turk J Agric For 26:211–216

    CAS  Google Scholar 

  • Habibi J (1975) The cotton whitefly Bemisia tabaci Gen.; biology and methods of control. Entomol Phytopath Appl 38:13–36

    Google Scholar 

  • Hajimorad MR, Kheyr-pour A, Alavi V, Ahoomanesh A, Bahar M, Rezaian MA, Gronenborn B (1996) Identification of whitefly transmitted tomato yellow leaf curl geminivirus from Iran and a survey of its distribution with molecular probes. Plant Path 45(3):418–425

    Article  Google Scholar 

  • Javan Moghaddam H (1993) Aspects of Bemisia tabaci in Iran and world. Proceedings of the 13th plant protection congress of Iran, Rasht, pp 300–307

  • Jones DR (2003) Plant viruses transmitted by whiteflies. Eur J plant pathol 9:95–219

    Google Scholar 

  • Legg JP, French R, Rogans D, Okao-Okuja G, Brown JK (2002) A distinct Bemisia tabaci (Gennadius) (Hemiptera: Sternorrhyncha: Aleyrodidae) genotype cluster is associated with the epidemic of severe cassava mosaic virus disease in Uganda. Mol Ecol 11:1219–1229

    Article  PubMed  CAS  Google Scholar 

  • Martin JH (1987) An identification guide to common whitefly species of the world. Trop Pest Manag 33:298–322

    Google Scholar 

  • Massumi H, Samei A, Hosseini Pour A, Shaabanian M, Rahimian H (2007) Occurrence, distribution, and relative incidence of seven viruses infecting greenhouse-grown cucurbits in Iran. Plant Dis 91(2):159–163

    Article  Google Scholar 

  • Mills LS, Allendorf FW (1996) The one migrant per generation rule in conservation and management. Entomol Conserv Biol 10:1509–1518

    Article  Google Scholar 

  • Mound LA (1963) Host-correlated variation in Bemisia tabaci (Gennadius) (Homoptera: Aleyrodidae). Proc Royal Entomol Soc London Ser 38:171–180

    Google Scholar 

  • Mound LA, Halsey SH (1978) Whitefly of the world: a systematic catalogue of the Aleyrodidae (Homoptera) with host plant and natural enemy data. Wiley, New York, p 340

    Google Scholar 

  • Moya A, Guirao P, Cifuentes D, Beitia F, Cenis JL (2001) Genetic diversity of Iberian populations of Bemisia tabaci (Hemiptera: Aleyrodidae) based on random amplified polymorphic DNA-polymerase chain reaction. Mol Ecol 10:891–897

    Article  PubMed  CAS  Google Scholar 

  • Murphy RW, Sites JW, Buth DG, Haufler CH (1996) Proteins: isozyme electrophoresis. In: Hillis DM, Moritz C, Mable BK (eds) Molecular systematics. Sinauer Associcates, Massachusetts, pp 51–120

    Google Scholar 

  • Nei M, Li WH (1979) Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci USA 76:5269–5273

    Article  PubMed  CAS  Google Scholar 

  • Nei M, Kumar S (2000) Molecular evolution and phylogenetic. Oxford University Press, New York, p 333

    Google Scholar 

  • Perring TM, Cooper AD, Rodriguez RJ, Farrar CA, Bellows TS Jr (1993) Identification of a whitefly species by genomic and behavioral studies. Science 259:74–77

    Article  PubMed  CAS  Google Scholar 

  • Qiu BLB, Coats SA, Ren SX, Idris AM, XU Caixia and Brown JK (2007) Phylogenetic analysis (mtCOI) and evidence for generalized polyphagy for the Bemisia tabaci (Genn.) complex (s.o. Homoptera: Aleyrodidae) endemic to Southeast Asia reveals a high-diversity hot spot, second only to Africa. Prog Nat Sci (in press)

  • Rosell RC, Bedford ID, Frohlich DR, Gill RJ, Markham PG, Brown JK (1997) Analyses of morphological variation in distinct populations of Bemisia tabaci. Ann Entomol Soc Am 90:575–589

    Google Scholar 

  • Russell LM (1957) Synonyms of Bemisia tabaci (Gennadius) (Homoptera: Aleyrodidae). Bull Brook Entomol Soc 52:122–123

    Google Scholar 

  • Samii MA (1993) Study of dispersal and comparative biology in population of Bemisia tabaci species complex and their genetic variation in Iran using RAPD-PCR marker. PhD thesis. Agriculture faculty, Tarbiat Modarres University

  • Schneider S, Roessli D and Excoffier L (2000) Arlequin ver 2.0: A software for population genetic data analysis. Univ of Geneva Genetic and Biometric Lab Geneva Switzerland

  • Simon C, Frati F, Beckenbach A, Crespi B, Liu H, Flook P (1994) Evolution, weighting, and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers. Ann Entomol Soc Am 87:651–701

    CAS  Google Scholar 

  • Sseruwagi P, Maruthi MN, Colvin J, Rey MEC, Brown JK, Legg JP (2006) Colonization of non-cassava plant species by cassava whiteflies (Bemisia tabaci) in Uganda. Entomol Exp Appl 119:145–153

    Article  CAS  Google Scholar 

  • Viscarret MM, Torres-Jerez I, Agostini de Manero E, López SN, Botto EE, Brown JK (2003) Mitochondrial DNA evidence for a distinct clade of New World Bemisia tabaci (Genn.) (Hemiptera:Aleyrodidae) from Argentina and Bolivia, and presence of the Old World B biotype in Argentina. Ann Entomol Soc Am 96:65–72

    Article  CAS  Google Scholar 

  • Wright S (1969) Evolution and the genetics of population. In: The Theory of Gene Frequencies vol 2. University of Chicago Press, Chicago

    Google Scholar 

  • Yousif MT, Kheyr-Pour A, Gronenborn B, Pitrat M, Dogimont C (2007) Sources of resistance to Watermelon Chlorotic Stunt Virus in melon. Plant Breed 126:4, 422–427

    Article  CAS  Google Scholar 

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Acknowledgments

We thank the staff of Insect Taxonomy Research Department, Iranian Research Institute of Plant Protection; Tehran, Iran for kind help, and colleagues of Cellular and Molecular Biology Research Center of Shahid Beheshti University, M.C., Tehran, Iran for useful comments.

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Authors and Affiliations

  1. Biology Department, Shahid Beheshti University, G.C., Tehran, Iran

    H. Rajaei Shoorcheh

  2. Cellular and Molecular Biology Research Center, Shahid Beheshti University, M.C., Tehran, Iran

    B. Kazemi

  3. Insect Taxonomy Research Department, Iranian Research Institute of Plant Protection, P.O. Box 1454, Tehran, 19395, Iran

    S. Manzari & A. Sarafrazi

  4. Department of Plant Sciences, University of Arizona, Tucson, AZ, 85721, USA

    J. K. Brown

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  1. H. Rajaei Shoorcheh
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  3. S. Manzari
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Correspondence to H. Rajaei Shoorcheh.

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Communicated by M. Traugott.

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Rajaei Shoorcheh, H., Kazemi, B., Manzari, S. et al. Genetic variation and mtCOI phylogeny for Bemisia tabaci (Hemiptera, Aleyrodidae) indicate that the ‘B’ biotype predominates in Iran. J Pest Sci 81, 199–206 (2008). https://doi.org/10.1007/s10340-008-0206-0

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