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Toxicity of biocide GCSC-BtA on arthropod pests under different temperature conditions

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Abstract

The present research dealt with the toxicity of GCSC-BtA (Germany–China Scientific Cooperation-Bacillus thuringiensis-Abamectin), a new type biocide developed by conjugating a toxin from Bacillus thuringiensis (B.t.) with Abamectin from Streptomyces avermitilis, on arthropod pests under different temperature conditions. The results showed that GCSC-BtA possessed higher toxicity than B.t. crystal, Abamectin or Cypermethrin, with pest mortalities of 97.9, 93.7, 96.4, 82.3 and 96.7% for Tetranychus cinnabarinus (Boisd.) (Acari, Tetranychidae), Frankliniella occidentalis Pergande (Thys., Thripidae), Aphis fabae Scopoli (Hom., Aphididae), Plutella xylostella (L.) (Lep., Plutellidae) and Cameraria ohridella Deschka et Dimić (Lep., Gracillariidae), respectively. Toxicities of GCSC-BtA to T. cinnabarinus and P. xylostella decreased significantly at the older developmental stage with an exception that egg stage had the highest tolerance, in which LC50s of GCSC-BtA were 0.0001, 0.0019 and 0.0708 mg/ml for nymph, adult and egg of T. cinnabarinus, and that 0.0399, 0.2035 and 0.9033 mg/ml for the 2nd instar larvae, 4th instar larvae and egg of P. xylostella, respectively. Also, the biocide was more effective to the lower stage of A. fabae than the higher one with LC50s of 0.0023, 0.0086 and 0.0171 mg/ml against 1st instar nymph, 3rd instar nymph and adult, respectively. In general, toxicity of the new type of biocide was positively related to temperature against all the tested pests, except against T. cinnabarinus, where it was almost similar at the three temperature conditions. GCSC-BtA displayed significantly lower toxicities at 15/20°C than 20/25°C or 25/30°C against F. occidentalis, A. fabae, P. xylostella and C. ohridella. However, no significant difference was found in the toxicities against F. occidentalis, A. fabae and C. ohridella above 20°C, whereas toxicity against P. xylostella increased as temperature raised. Therefore, to achieve higher efficacy in the field, application rate and time should be devised according to these factors.

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Acknowledgements

The authors are grateful to the Deutsche Forschungsgemeinschaft (DFG) and BMZ of Germany and National Natural Science Foundation of China (NSFC) (PN: 30471175) for their supports.

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Authors and Affiliations

  1. Department of Entomology and Plant Protection, Institute of Phytopathology, University of Bonn, Nussallee 9, 53115, Bonn, Germany

    Y. J. Zhu & C. Sengonca

  2. Biotechnology Center, Fujian Academy of Agricultural Sciences, Fuzhou, 350003, Fujian, People’s Republic of China

    B. Liu

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  1. Y. J. Zhu
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  2. C. Sengonca
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  3. B. Liu
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Correspondence to C. Sengonca.

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Communicated by Andreas Vilcinskas

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Zhu, Y.J., Sengonca, C. & Liu, B. Toxicity of biocide GCSC-BtA on arthropod pests under different temperature conditions. J Pest Sci 79, 89–94 (2006). https://doi.org/10.1007/s10340-005-0118-1

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  • DOI: https://doi.org/10.1007/s10340-005-0118-1

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