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Comprehensive Quantification of Miconazole Nitrate, Mupirocin, and Mometasone Furoate: a Dual Analysis via HPLC and HPTLC with Comparative Evaluation Against Greenness Parameters

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Abstract

Combination treatment enhances efficacy, minimizes side effects, and improves convenience for patients with diverse treatment schedules. This compilation focuses on the simultaneous estimation of Miconazole nitrate (MNE), Mupirocin (MRN), and Mometasone furoate (MMT) using HPLC and HPTLC in an ointment which is wisely used combination for treatment of skin disorders. The optimized isocratic HPLC method consists of a C18 column, 0.02% triethylamine: methanol (15:85; % v/v), at a flow rate of 0.9 mL/min. The retention times were found to be 4.716 ± 0.082 min for MNE, 13.716 ± 0.188 min for MRN, and 2.241 ± 0.034 min for MMT using the developed method. The method was validated indetail and the quantification range found 20–180 µg/mL for both MNE and MRN and 1–9 µg/mL for MMT. In an alternative HPTLC method, toluene, ethyl acetate, and ethanol (10:3:2; v/v/v) were used as mobile phase on silica gel 60F254-coated aluminum sheets, with scanning at 240 nm. The MNE, MRN and MMT drugs spot eluted at Rf values of 0.65, 0.36, and 0.80, respectively. In-depth validation was performed and assessed statistically, including, specificity, precision, accuracy, reproducibility, and accuracy. The HPTLC method found linear in ranges of 1200–5200 ng/band for MNE and MRN, and 60–260 ng/band for MMT. Consequently, these developed chromatographic methods assessed according to green analytical principle and found sustainable. The developed methods can be effectively applied for the quantitative analysis of commercially available dosage forms.

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All the data generated or analysed during this study are included in this published article.

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Author notes

  1. Aarti Sachin Zanwar and Anuj N. Nahata are First authors and Contributed equally.

Authors and Affiliations

  1. Shobhaben Pratapbhai Patel School of Pharmacy and Technology Management, SVKMs NMIMS-Deemed to Be University, V.L. Mehta Road, Vile Parle (W), Mumbai, Maharashtra, 400056, India

    Anuj N. Nahata & Mital Patel

  2. Department of Pharmacy, Sumandeep Vidyapeeth Deemed to Be University, Piparia, Waghodia, Vadodara, Gujarat, 391760, India

    Aarti Sachin Zanwar, Ashim Kumar Sen & Dhanya B. Sen

  3. Faculty of Pharmacy, The Maharaja Sayajirao University of Baroda, Vadodara, 390001, Gujarat, India

    Sachin Zanwar

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  1. Aarti Sachin Zanwar
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Contributions

Aarti Sachin Zanwar, Ashim Kumar Sen, Dhanya B Sen, Sachin Zanwar: Writing – original draft, Material preparation, data collection and analysis, Validation, Investigation, Formal analysis. Anuj N. Nahata: Writing – original draft, GREEnness Assessment. Mital Patel: Review and final editing, supervision, project administration, conceptualization.

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Correspondence to Mital Patel.

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Zanwar, A.S., Nahata, A.N., Sen, A.K. et al. Comprehensive Quantification of Miconazole Nitrate, Mupirocin, and Mometasone Furoate: a Dual Analysis via HPLC and HPTLC with Comparative Evaluation Against Greenness Parameters. Chromatographia (2024). https://doi.org/10.1007/s10337-024-04338-8

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