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Identification and Quantification of Characteristic Peptides (Oligopeptides) in Shuxuetong (SXT) Injection by LC–MS/MS

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Abstract

The prevalence of cerebrovascular disease (CVD) is increasing worldwide. SXT injection is commonly used for the treatment of CVD in China. First, Identification of Peptides in SXT by nano-LC–MS/MS. Further, the standards of nine peptides of SXT injection were analyzed by liquid chromatography–mass spectrometry (LC–MS/MS). A LC–MS/MS was established for determining the content of the four peptides in SXT injection. The results showed that there were differences in the content of the four polypeptide components. This method provides a reference for the quality control of SXT injection. This research strategy also provides a reference for the structural characterization, content determination in other complex systems and quality control of related drugs.

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Acknowledgements

This work was supported by the National Key R&D Program of China (2021YFC1712900) and the Tianjin Research Innovation Project for Postgraduate Students (2021YJSB287). Moreover, we would also like to acknowledge YouBo Pharmaceutical Co., Ltd for freely providing the drugs used in this work.

Funding

This work was supported by the National Natural Science Foundation of China (grant no. 81303182, 81173523) and the National Key R&D Program of China (2021YFC1712900).

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Author notes

  1. Zhenyan Yu, Xiaomei Liu and Yanchao Xing are co-first authors.

Authors and Affiliations

  1. Tianjin University of Traditional Chinese Medicine, Tianjin, China

    Zhenyan Yu, Xiaomei Liu, Yanchao Xing, Xiaoming Wang, Xianrui Wang & Lifeng Han

  2. Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, 300250, China

    Yuhong Huang & Guixiang Pan

Authors
  1. Zhenyan Yu
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  2. Xiaomei Liu
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  3. Yanchao Xing
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  4. Xiaoming Wang
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  6. Yuhong Huang
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by ZY, XH and YX. The first draft of the manuscript was written by ZY and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Guixiang Pan.

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Yu, Z., Liu, X., Xing, Y. et al. Identification and Quantification of Characteristic Peptides (Oligopeptides) in Shuxuetong (SXT) Injection by LC–MS/MS. Chromatographia 85, 1029–1039 (2022). https://doi.org/10.1007/s10337-022-04201-8

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