Abstract
Nicotiana tabacum was found to have the potent anti-herbivore defense capacity by secondary metabolites of 17-hydroxygeranyllinalool diterpene glycosides (HGL-DTGs). A heart-cutting two-dimensional LC coupled with tandem MS method was developed to analysis the HGL-DTGs. And 10 types of HGL-DTGs were determined qualitatively and quantitatively. In the novel method, reversed-phase liquid chromatography (RPLC) had been employed in the first dimension for separation of HGL-DTGs with other interference compounds. Then target elution, including HGL-DTGs, was cut to the trap column by online dilution from the additional pump. The analytes of HGL-DTGs were reserved and enriched on the trap column. After switch of the 6-port valve, the analytes were eluted to the second dimensional separation. The HGL-DTGs were then determined by the tandem MS under the multiple reaction monitoring mode (MRM). The intra-day and inter-day RSD of the HGL-DTGs were between 0.24 and 7.21%. Consequently, the new method was successfully utilized to detect the HGL-DTGs levels in a wide range of the tobacco samples, which showed fairly good accuracy and reproducibility. The new strategy offered a simple and fast way for further investigation of the HGL-DTGs in Nicotiana tabacum.
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Abbreviations
- 2D-LC–MS/MS:
-
Two-dimensional liquid chromatography–tandem mass spectrometry;
- LC-LC:
-
Heart-cutting
- RPLC:
-
Reversed-phase liquid chromatography;
- HGL-DTGs:
-
17-Hydroxygeranyllinalool diterpene glycosides;
- MRM:
-
Multiple reaction monitoring;
- ACN:
-
Acetonitrile
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Chen, M., Wang, T., Wang, W. et al. Analysis of 17-Hydroxygeranyllinalool Diterpene Glycosides in Nicotiana tabacum by Using Heart-Cutting 2D-LC Coupled with Tandem MS Technique. Chromatographia 85, 931–937 (2022). https://doi.org/10.1007/s10337-022-04188-2
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DOI: https://doi.org/10.1007/s10337-022-04188-2