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Achieving Higher Signal Response Than Splitless GC Injection by High-Pressure Headspace Sampling and Full Evaporation Technique

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Abstract

This paper aims to improve the signal response in headspace (HS) analysis over than the GC analysis with a classical splitless injection mode, and this was achieved by the development of high-pressure headspace (HP-HS) analysis method. Based on the theoretical analysis on the sampling principle of HS, the HP-HS concept was proposed in section one, indicative of possibility to achieve excellent signal response. In the proposed HP-HS technology, the complete transfer of gas sample from HS to GC column is achieved in a very short purge-off time in the splitless injection of GC using the high-pressure carrier gas. The sampling size was increased by adopting high-pressure auxiliary gas. The dilution effect of venting process on detected substance was greatly reduced by controlling the terminal pressure of venting process at a high level. With the HP-HS analysis technology, the optimal equilibration temperature and signal response for analytes with high or medium volatility in ten common solvents was determined, and the results showed that the HP-HS-GC method can achieve very high signal response and very low solvent effect on GC. For aqueous samples, the signal response of HP-HS-GC can be up to three times than that of the splitless GC analysis. The present technology has a great potential, in particular, in high sensitivity analysis of complicated samples with serious substrate effect.

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Acknowledgements

The authors acknowledge the Natural Science Foundation of China (31700507, 21576105), the National Key Research and Development Program of China (2017YFB0307900), and the FAFU’s Fund for Distinguished Young Scholars (XJQ201601) to sponsor this research.

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Authors and Affiliations

  1. College of Material Engineering, Fujian Agriculture and Forestry University, Fuzhou, 350002, China

    Jiawei Yang, Tong Zeng, Huimin Xu, Yonghao Ni, Liulian Huang, Lihui Chen & Hui-Chao Hu

  2. Limerick Pulp and Paper Centre, University of New Brunswick, P. O. Box 4400, Fredericton, Canada

    Yonghao Ni

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  1. Jiawei Yang
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  2. Tong Zeng
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  3. Huimin Xu
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  4. Yonghao Ni
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  7. Hui-Chao Hu
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Correspondence to Hui-Chao Hu.

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Yang, J., Zeng, T., Xu, H. et al. Achieving Higher Signal Response Than Splitless GC Injection by High-Pressure Headspace Sampling and Full Evaporation Technique. Chromatographia 85, 507–517 (2022). https://doi.org/10.1007/s10337-022-04161-z

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  • DOI: https://doi.org/10.1007/s10337-022-04161-z

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