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Optimization of an Alkaline Hydrolysis Preparation of Capilliposide-A from Lysimachia capillipes Hemsl. Using Response Surface Methodology Coupled with HPLC-ELSD Analysis

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Abstract

Capilliposide-A, a rare secondary saponin found in Lysimachia capillipes, has been reported to exhibit good biological activities. However, it is difficult to obtain a sufficient of capilliposide-A for further research through column chromatography and chemical synthesis. The aim of this work was to establish an efficient approach for the convenient preparation of this steroid saponin based on alkaline hydrolysis. The hydrolysis conditions were optimized by response surface methodology after a preliminary investigation of the affecting factors by single-factor experiments. Under the optimal conditions, the macroporous resin that adsorbed capilliposide-B and capilliposide-C was hydrolyzed in an 8% (w/v) NaOH solution at 35 °C for 65 h, and the yield of capilliposide-A was 68.90%. The results demonstrated that this newly developed approach is efficient for the preparation of capilliposide-A, and this approach is also crucial for further development and clinical applications.

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Acknowledgements

This work was supported by the Science and Technology Develop Project of Changshu (CS202014).

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Authors and Affiliations

  1. College of Biomedical Engineering and Instrument Science, Zhejiang University, Hangzhou, 310027, China

    Wei Li, Xiaoyong Zhang, Xuezhao Chen, Zhuoheng Zhong & Jingkui Tian

  2. Institute of Cancer and Basic Medicine, Chinese Academy of Sciences, Cancer Hospital of the University of Chinese Academy of Sciences, Zhejiang Cancer Hospital, Hangzhou, 310002, China

    Wei Li, Wei Zhu, Shouxin Li & Jingkui Tian

  3. Changshu Qiushi Technology Co., Ltd, Suzhou, 215500, China

    Shouxin Li

  4. School of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, China

    Xin Pan

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  1. Wei Li
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  2. Xiaoyong Zhang
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  5. Wei Zhu
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Correspondence to Jingkui Tian.

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Li, W., Zhang, X., Chen, X. et al. Optimization of an Alkaline Hydrolysis Preparation of Capilliposide-A from Lysimachia capillipes Hemsl. Using Response Surface Methodology Coupled with HPLC-ELSD Analysis. Chromatographia 85, 167–175 (2022). https://doi.org/10.1007/s10337-021-04124-w

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  • DOI: https://doi.org/10.1007/s10337-021-04124-w

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