Skip to main content
SpringerLink
Log in

Quantitative UPLC-MS/MS to Detect DMPC and DPPC Applied to Paraquat Poisoning in Cells and Serum

  • Original
  • Published:
Chromatographia Aims and scope Submit manuscript

Abstract

Background

1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and 1,2-dipalmitoyl-sn-glycerol-3-phosphorylcholine (DPPC) are two common phosphatidylcholines that are the critical components of bilayer membrane structure.

Methods

To detect DMPC and DPPC, an ultra-high-performance liquid chromatography–mass spectrometry (UPLC-MS/MS) method was established. DMPC and DPPC were separated on an amide column with gradient elution and determined by multiple reaction monitoring at m/z 678.6 \(\to\) 184.3 (DMPC) and 734.5 \(\to\) 184.03 (DPPC).

Results

The method was validated and applied to determine the concentration of DMPC and DPPC in paraquat (PQ)-poisoned cells and serum. DMPC and DPPC showed a good linear relationship in both extracellular media. The precision and accuracy of DMPC and DPPC were within 15%. The extracellular concentrations of DMPC and DPPC increased gradually from 12 to 72 h. Spearman analysis of DMPC and DPPC showed that the correlation coefficient was 0.774 in the control group and 0.621 in the PQ group. In conclusion, a rapid and specific UPLC-MS/MS method for detecting DPPC and DMPC was developed and validated. There was a significant correlation between DMPC and DPPC. PQ poisoning decreased the correlation between DPPC and DMPC.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

Data availability

The datasets generated and/or analyzed during the current study are not publicly available but are available from the corresponding author on reasonable request.

References

  1. Zhou L, Zhao M, Ennahar S, Bindler F, Marchioni E (2012) Anal Bioanal Chem 403:291–300. https://doi.org/10.1007/s00216-012-5772-6

    Article  CAS  PubMed  Google Scholar 

  2. Lind TK, Darre L, Domene C, Urbanczyk-Lipkowska Z, Cardenas M, Wacklin HP (2015) Biochim Biophys Acta 1848:2075–2084. https://doi.org/10.1016/j.bbamem.2015.05.015

    Article  CAS  PubMed  Google Scholar 

  3. Zawisza I, Wittstock G, Boukherroub R, Szunerits S (2008) Langmuir 24:3922–3929. https://doi.org/10.1021/la703651n

    Article  CAS  PubMed  Google Scholar 

  4. Shafique N, Kennedy KE, Douglas JF, Starr FW (2016) J Phys Chem B 120:5172–5182. https://doi.org/10.1021/acs.jpcb.6b02982

    Article  CAS  PubMed  Google Scholar 

  5. Zhou L, Narsimhan G, Wu X, Du F (2014) Colloids Surf B Biointerfaces 123:419–428. https://doi.org/10.1016/j.colsurfb.2014.09.037

    Article  CAS  PubMed  Google Scholar 

  6. Lyu Y, Fitriyanti M, Narsimhan G (2019) Colloids Surf B Biointerfaces 173:121–127. https://doi.org/10.1016/j.colsurfb.2018.09.049

    Article  CAS  PubMed  Google Scholar 

  7. Langner M, Hui S (2000) Biochim Biophys Acta 1463:439–447. https://doi.org/10.1016/s0005-2736(99)00236-9

    Article  CAS  PubMed  Google Scholar 

  8. Severcan F, Baykal U, Suzer S (1996) Anal Bioanal Chem 355:415–417. https://doi.org/10.1007/s0021663550415

    Article  CAS  PubMed  Google Scholar 

  9. Puhse M, Jeworrek C, Winter R (2008) Chem Phys Lipids 152:57–63. https://doi.org/10.1016/j.chemphyslip.2008.01.002

    Article  CAS  PubMed  Google Scholar 

  10. Lopes S, Simeonova M, Gameiro P, Rangel M, Ivanova G (2012) J Phys Chem B 116:667–675. https://doi.org/10.1021/jp210088n

    Article  CAS  PubMed  Google Scholar 

  11. Corrales Chahar F, Diaz SB, Ben Altabef A, Gervasi CA, Alvarez PE (2019) Chem Phys Lipids 218:125–135. https://doi.org/10.1016/j.chemphyslip.2018.12.010

    Article  CAS  PubMed  Google Scholar 

  12. Domracheva I, Muhamadejev R, Petrova M, Liepinsh E, Gulbe A, Shestakova I, Duburs G, Arsenyan P (2015) Toxicol Rep 2:377–383. https://doi.org/10.1016/j.toxrep.2015.01.009

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. El Khoury ED, Patra D (2013) J Phys Chem B 117:9699–9708. https://doi.org/10.1021/jp4061413

    Article  CAS  PubMed  Google Scholar 

  14. Siwy CM, Delfing BM, Smith AK, Klimov DK (2020) J Chem Inf Model 60:4030–4046. https://doi.org/10.1021/acs.jcim.0c00590

    Article  CAS  PubMed  Google Scholar 

  15. Makyla-Juzak K, Chachaj-Brekiesz A, Dynarowicz-Latka P, Dabczynski P, Zemla J (2018) J Membr Biol 251:641–651. https://doi.org/10.1007/s00232-018-0041-z

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  16. Zhang Z, Huang B, Wang X, Hu L (2020) Lat Am J Pharm 39:2502–2506

    CAS  Google Scholar 

  17. Suntres ZE (2002) Toxicology 180:65–77. https://doi.org/10.1016/s0300-483x(02)00382-7

    Article  CAS  PubMed  Google Scholar 

Download references

Funding

This work was supported by fund of the Zhejiang Provincial Natural Science Foundation of China (LY19H230001), Wenzhou Science and Technology Bureau (Y20180106), the Health Department of Zhejiang province (2021KY781).

Author information

Authors and Affiliations

  1. Department of Pharmacy, Wenzhou Central Hospital, Wenzhou, 325000, China

    Lichuan Hu

  2. Department of Pharmacy, The First Affiliated Hospital of Wenzhou Medical University, Nanbaixiang Street, Ouhai District, Wenzhou, 325000, China

    Guofeng Xie, Qin Lan, Zheng Yu & Lufeng Hu

  3. Department of Pathology, Integrated Chinese and Western Medicine Hospital of Wenzhou, Wenzhou, China

    Lihe Zhu

Authors
  1. Lichuan Hu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Guofeng Xie
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Qin Lan
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Zheng Yu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Lufeng Hu
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Lihe Zhu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Lufeng Hu or Lihe Zhu.

Ethics declarations

Conflict of interest

The authors declare no conflict of interest.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary Information

Below is the link to the electronic supplementary material.

Supplementary file1 (DOCX 87 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Hu, L., Xie, G., Lan, Q. et al. Quantitative UPLC-MS/MS to Detect DMPC and DPPC Applied to Paraquat Poisoning in Cells and Serum. Chromatographia 85, 147–153 (2022). https://doi.org/10.1007/s10337-021-04113-z

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10337-021-04113-z

Keywords

Search

Navigation