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Oligosaccharide Profile Analysis and Quality Control of Atractylodes macrocephala Koidz. Using HPLC-HRMS/MS and a Simple HPLC-ELSD Method

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Abstract

Oligosaccharides in Atractylodes macrocephala Koidz. (AMK) have a wide range of clinical effects, especially in terms of improving immunity. In this study, the oligosaccharide fingerprint of AMK was first established by high-performance liquid chromatography coupled with evaporative light-scattering detection. This method was confirmed to be accurate and reliable. Twenty-six batches of samples from four regions were detected, and the oligosaccharide fingerprint similarity of AMK was evaluated (> 0.900). Second, the oligosaccharide profile of AMK was analyzed using high-performance liquid chromatography coupled to high-resolution tandem mass spectrometry. Fourteen inulin-type oligosaccharides with a degree of polymerization of 2–15 were identified, including sucrose, 1-kestose, and nystose. Finally, two representative compounds, 1-kestose and nystose, were selected for quantification analysis. The established method had good regression equations, precision, repeatability, and stability. The average contents of 1-kestose and nystose were 0.688% and 0.827%, respectively. This study provides valuable information on the quality evaluation and discrimination of different varieties of AMK.

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Acknowledgements

This study was financially supported by National Development and Reform Commission standardization project of traditional Chinese medicine (NO: ZYBZH-C-JS-33). Thank you for the help in instrumental analysis provided by Center for Instrumental Analysis of Nanjing University of Chinese Medicine.

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Authors and Affiliations

  1. School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, People’s Republic of China

    Na Liu, Yi Shu, Yin-yu Yan, Guo-ping Peng, Hong-mei Wen, Chen-xiao Shan, Xiao-bin Cui & Xin-zhi Wang

  2. Anhui Jiren Pharmaceutical Company Limited., Bozhou, 236800, Anhui, People’s Republic of China

    Cheng-bin Zuo & Xiang-yu Li

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  1. Na Liu
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Correspondence to Hong-mei Wen.

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Liu, N., Shu, Y., Yan, Yy. et al. Oligosaccharide Profile Analysis and Quality Control of Atractylodes macrocephala Koidz. Using HPLC-HRMS/MS and a Simple HPLC-ELSD Method. Chromatographia 85, 47–54 (2022). https://doi.org/10.1007/s10337-021-04107-x

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