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Modeling and Optimization of Chromatographic Purification of Arglabin from CO2 Extract of Artemisia glabella Kar. et Kir.

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Abstract

The dried aerial parts of the plant Artemisia glabella Kar. et Kir. contain about 1.1% of the very important sesquiterpene lactone arglabin. This compound can be extracted by supercritical CO2 giving a 20 time concentrated extract containing about 22% arglabin. The CO2 extract can be efficiently treated by hydrostatic countercurrent chromatography (CCC) able to produce in a single run 95% pure arglabin. Both mobile and stationary phase are liquid in CCC. The biphasic waterless liquid system heptane:ethyl acetate:acetonitrile 2:1:2 v/v/v was used to separate arglabin from its two major co-extracted compounds: argolide and eucalyptol. A three-factor Box–Behnken design was used to optimize the CCC preparative purification of the CO2 extract by a FCPC-5L instrument. Injected amount, mobile phase flow rate, and rotor rotation speed were used to optimize arglabin productivity. An optimized run was able to treat 90 g of extract producing 20 g of 95% pure arglabin in 50 min, a theoretical productivity of 400 mg/min.

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Funding

The work was carried out under the grant project AP08052030 “Modeling and optimization of the technology of original drugs” funded by the Science Committee of the Ministry of Education and Science of the Republic of Kazakhstan.

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Authors and Affiliations

  1. JSC “International Research and Production Holding “Phytochemistry”, Karaganda, Republic of Kazakhstan

    S. M. Adekenov, I. A. Khabarov, G. A. Yakovenko & A. S. Adekenova

  2. Institut des Sciences Analytiques, CNRS, Université de Lyon 1, Villeurbanne, France

    A. Berthod

Authors
  1. S. M. Adekenov
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  2. I. A. Khabarov
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  3. G. A. Yakovenko
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  4. A. S. Adekenova
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  5. A. Berthod
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Correspondence to S. M. Adekenov.

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Adekenov, S.M., Khabarov, I.A., Yakovenko, G.A. et al. Modeling and Optimization of Chromatographic Purification of Arglabin from CO2 Extract of Artemisia glabella Kar. et Kir.. Chromatographia 84, 1077–1086 (2021). https://doi.org/10.1007/s10337-021-04093-0

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  • DOI: https://doi.org/10.1007/s10337-021-04093-0

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