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Evaluation of Poly(glycidyl methacrylate)-Coated Column for Enantioseparation with Azithromycin Lactobionate and Clindamycin Phosphate as Chiral Selectors in Capillary Electrophoresis

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Abstract

In this study, a poly(glycidyl methacrylate) nanoparticle (PGMA NP)-coated column system with two antibiotics as selector was constructed for enantioseparation. The PGMA NP coating inside the capillary columns could be easily introduced by a simple ring open reaction. Five basic drugs were used as the model to evaluate the enantioselectivity of Azithromycin Lactobionate (AL) and Clindamycin phosphate (CP)-based separation system. Factors that influence the chiral separation resolution were systematically investigated, such as chiral selector concentration, background electrolyte (BGE) pH, applied voltage and system temperature. Under the optimized conditions, improved enantioseparations were obtained for all enantiomers and the maximum column efficiency of model drugs could reach 120,000 plates/m. The repeatability of relative standard deviations for EOF representing run-to-run, day-to-day and column-to-column was less than 4.32%. In addition, molecular modeling with AutoDock was applied to elucidate the explored potential mechanism of AL/CP for recognizing racemic drugs.

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Abbreviations

AL:

Azithromycin lactobionate

APTS:

3-Aminopropyltriethoxysilane

ATE:

Atenolol

CHL:

Chlorphenamine

CP:

Clindamycin phosphate

ESM:

Esmolol

GMA:

Glycidyl methacrylate

NEF:

Nefopam

PRO:

Propranolol

PGMA:

Poly(glycidyl methacrylate)

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Acknowledgements

This study was funded by the National Natural Science Foundation of China (No. 82003705), the National Key Research and Development Program of China (No. 2016YFD0501101) and the project of Food Science Discipline Construction of Shanghai University and the National Natural Science Foundation of China (No. 31201306).

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Authors and Affiliations

  1. School of Medicine, Shanghai University, Shanghai, 200444, People’s Republic of China

    Xiaodong Sun

  2. School of Life Sciences, Shanghai University, Shanghai, 200444, People’s Republic of China

    Qin Chen

  3. Key Laboratory of Drug Quality Control and Pharmacovigilance (Ministry of Education), China Pharmaceutical University, Nanjing, 210009, People’s Republic of China

    Wen Ding, Cheng Chen, Tao Yu & Ke Yang

  4. State Key Laboratory of Natural Medicines, China Pharmaceutical University, No. 24 Tongjiaxiang, Nanjing, 210009, Jiangsu, People’s Republic of China

    Wen Ding, Cheng Chen, Tao Yu & Ke Yang

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  1. Xiaodong Sun
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  3. Cheng Chen
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Correspondence to Qin Chen or Ke Yang.

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Sun, X., Ding, W., Chen, C. et al. Evaluation of Poly(glycidyl methacrylate)-Coated Column for Enantioseparation with Azithromycin Lactobionate and Clindamycin Phosphate as Chiral Selectors in Capillary Electrophoresis. Chromatographia 84, 499–505 (2021). https://doi.org/10.1007/s10337-021-04029-8

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  • DOI: https://doi.org/10.1007/s10337-021-04029-8

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