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Rapid Detection of Short-Chain Fatty Acids in Biological Samples


An increasing number of researchers are focusing on short-chain fatty acids (SCFAs), which are closely related to various aspects of the human body. This study presented a pretreatment method for mouse feces that combined acidification and extraction. Then, a direct injection process into gas chromatography was developed. The limits of detection using this method are 0.5–0.8 mg L−1, and the corresponding sample contents are 0.015–0.024 mg g−1. The limits of quantification are 0.8–1.0 mg L−1, and the corresponding sample contents are 0.024–0.030 mg g−1. The linear relationship of the calibration curve is good and has a correlation coefficient of > 0.999. The recovery rate of the spiked standard is 80–102%. This method had been successfully applied to analysing and determining SCFAs in biological samples.

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This work has been supported by the Foundation (No. ZXKF20180304) of Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control.


This work has been supported by the Foundation (No. ZXKF20180304) of Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control.

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Correspondence to Chaozheng Zhang or Hua Zhao.

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Zhang, C., Fan, L. & Zhao, H. Rapid Detection of Short-Chain Fatty Acids in Biological Samples. Chromatographia 83, 305–310 (2020).

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  • Short-chain fatty acids
  • Acidification
  • Extraction
  • Gas chromatography
  • Biological samples