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Characterization of Lipstatin and the Minor Components from Streptomyces toxytricini Fermentation Broth by HPLC–ESI–Q-TOF–MS

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Abstract

As the major product, lipstatin was originally isolated from the fermentation broth of Streptomyces toxytricini. Moreover, several lipstatin analogs are also produced in the fermentation process. In this study, a high-performance liquid chromatography coupled with electrospray ionization–quadrupole time-of-flight–mass spectrometry (HPLC–ESI–Q-TOF–MS) method was established for rapid separation and identification of multiple constituents in the fermentation broth of Streptomyces toxytricini. Based on the systematic investigation of lipstatin fragmentation pathways, 7 lipstatin analogs were identified as hydroxylipstatin (1), dehydrolipstatin (2, 4), seco-lipstatin (3), desmethyllipstatin (5), methyllipstatin (7), and dihydrolipstatin (8) for the first time. This study enriches the chemical profiling of fermentation broth of Streptomyces toxytricini and could provide valuable information for the biosynthetic study and the separation of lipstatin and further investigation of the preparation of tetrahydrolipstatin.

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Funding

This work was funded by the Science and Technology Planning Project of Fujian Province (2018R1009-12)

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Authors and Affiliations

  1. Fujian Institute of Microbiology, No. 25 Jinbu Road, Cangshan, Fuzhou, 350007, Fujian, China

    Huang-jian Yang, Zhu-lan Zhang, Ling-bin Yan, Xian Cheng, Zhou-qin Chen, De-sen Wang & Yun-yang Lian

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  1. Huang-jian Yang
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  2. Zhu-lan Zhang
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  5. Zhou-qin Chen
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Correspondence to Huang-jian Yang or Yun-yang Lian.

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Yang, Hj., Zhang, Zl., Yan, Lb. et al. Characterization of Lipstatin and the Minor Components from Streptomyces toxytricini Fermentation Broth by HPLC–ESI–Q-TOF–MS. Chromatographia 82, 1791–1800 (2019). https://doi.org/10.1007/s10337-019-03807-9

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  • DOI: https://doi.org/10.1007/s10337-019-03807-9

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