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Simultaneous Determination of Three Herbicides in Honey Samples Using an Aqueous Biphasic System Coupled with HPLC–MS/MS

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Abstract

A simple and environmentally friendly method was developed for simultaneous determination of residue from three triazine herbicides (simetryn, prometryn, and atrazine) from honey samples. The selected herbicides were extracted and purified using an aqueous biphasic system (ABS) based on an ethylene oxide/propylene oxide copolymer (EOPO-2500) and choline acetate ([Ch][Ac]), and determined by HPLC–MS/MS. The partitioning behavior of the analytes using the ABS was investigated, and the analytes mainly concentrated in the copolymer phase. At three spiked levels (2.0, 20.0, 100.0 μg kg−1), the recoveries of the analytes were determined in the range of 81–108%, with relative standard deviations no higher than 10.5%. The limits of quantification for the herbicides ranged from 0.1 to 1.5 µg kg−1. The proposed method is very promising for sample preparation, taking into account its simple operation, mild conditions, and biocompatibility, without the use of any organic solvents during the extraction process.

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Acknowledgements

This project was financially supported by the National Key Research and Development project of China (grant no. 2017YFD08007001). The authors would like to acknowledge Dr. Robin D. Rogers of The University of Alabama for his support in the research of the aqueous biphasic system.

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Authors and Affiliations

  1. College of Plant Protection, Shenyang Agricultural University, 120 Dongling Road, Shenyang, 110161, People’s Republic of China

    Hongzhe Tian, Haoliang Fu, Chunqi Xu & Chenghao Xu

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  1. Hongzhe Tian
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  2. Haoliang Fu
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  3. Chunqi Xu
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  4. Chenghao Xu
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Correspondence to Hongzhe Tian.

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Tian, H., Fu, H., Xu, C. et al. Simultaneous Determination of Three Herbicides in Honey Samples Using an Aqueous Biphasic System Coupled with HPLC–MS/MS. Chromatographia 82, 1571–1577 (2019). https://doi.org/10.1007/s10337-019-03781-2

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