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Detection of 2,2′-Azobis(2-amidinopropane) Dihydrochloride in Polyvinylpyrrolidone by Capillary Electrophoresis with Field-Amplified Sample Injection

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Abstract

A sensitive method was developed for detecting 2,2′-azobis(2-amidinopropane) dihydrochloride in polyvinylpyrrolidone by capillary electrophoresis with indirect UV detection. The detection sensitivity was improved significantly using the field-amplified sample injection technique. The background electrolyte was composed of 25 mM imidazole-HCl solution (pH 4.0), in which imidazole acted as the cation probe for the indirect detection at UV 214 nm. Effects of the experimental parameters including pH, the concentration of imidazole as well as the sample injection time on sample preconcentration were investigated and optimized. Under the optimized conditions, baseline separation of 2,2′-azobis(2-amidinopropane) ion with other ions in the polyvinylpyrrolidone samples was achieved within 8 min. The RSD% for the repeatability of the migration time and the peak area were determined as 0.95% and 3.47%, respectively. The limit of detection (LOD, S/N = 3) and the limit of quantitation (LOQ, S/N = 10) were determined as 1.11 × 10−5 M and 2.77 × 10−5 M, respectively. The measured recovery at three concentration levels was in the range of 101–104%. This method was successfully applied to detect 2,2′-azobis(2-amidinopropane) in the commercial polyvinylpyrrolidone samples.

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Acknowledgements

This work was financially supported by the National Natural Science Foundations of China (21775158, 21375140, 21175146) and the Strategic Priority Research Program of the Chinese Academy of Sciences (Grant No. XDB20020200).

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Authors and Affiliations

  1. School of Physical Science and Technology, ShanghaiTech University, Haike Road 100, Shanghai, 200120, China

    Chunli Lou & Jingwu Kang

  2. State Key Laboratory of Bioorganic and Natural Products Chemistry, Center for Excellence in Molecular Synthesis, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, 345 Lingling Lu, Shanghai, 200032, China

    Chunli Lou, Mengmeng Zheng, Yao Xu, Yuting Shen & Jingwu Kang

  3. University of Chinese Academy of Sciences, Yuquan Road 19, Beijing, 100049, China

    Chunli Lou, Mengmeng Zheng, Yao Xu, Yuting Shen & Jingwu Kang

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  1. Chunli Lou
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  2. Mengmeng Zheng
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  3. Yao Xu
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  4. Yuting Shen
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Correspondence to Jingwu Kang.

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Lou, C., Zheng, M., Xu, Y. et al. Detection of 2,2′-Azobis(2-amidinopropane) Dihydrochloride in Polyvinylpyrrolidone by Capillary Electrophoresis with Field-Amplified Sample Injection. Chromatographia 82, 1579–1583 (2019). https://doi.org/10.1007/s10337-019-03765-2

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  • DOI: https://doi.org/10.1007/s10337-019-03765-2

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