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Development and Validation of HPLC–MS/MS Method for the Simultaneous Determination of 8-Hydroxy-2′-deoxyguanosine and Twelve Cosmetic Phenols in Human Urine

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Abstract

A simple and sensitive method based on liquid–liquid extraction (LLE) and liquid chromatography electrospray ionization tandem mass spectrometry (LC–ESI–MS/MS), was developed and validated for the simultaneous determination of 8-hydroxy-2′-deoxyguanosine and twelve cosmetic phenols, including five benzophenone (BP)-type ultraviolet (UV) filters, five parabens, bisphenol A (BPA) and triclosan (TCS) in human urine. Urine samples were treated by a combination of enzymatic deconjugation with β-glucuronidase/arylsulfatase and extraction with ethyl acetate. The efficiencies of different extraction procedures were investigated. Both chromatographic and mass spectrometric conditions were optimized. As a result, under the optimized conditions, the limits of detection (LODs) for the analytes in the range from 0.01 to 0.23 μg/L were achieved. The intra-day and inter-day precisions were within the range of 0.8–6.1% and 1.8–9.5% (relative standard deviation, RSD), respectively. The correlation coefficients (R2) and recoveries for the analytes were ranged from 0.991–0.999 and 80.0–108%, respectively. The developed method initially combines the simultaneous analysis of urinary 8-hydroxy-2′-deoxyguanosine, BP-type UV filters, parabens, BPA and TCS, and was successfully applied to urine samples from children in South China.

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Acknowledgements

This work was supported by grants from the Natural Science Foundation of Guangdong Province, China (GDNSFC project no. 2015A030313869), the Guangzhou Key Laboratory of Environmental Exposure and Health (No. GZKLEEH201610), and the Shenzhen Municipal Government Research Projects (nos. JCYJ20160428143348745, JCYJ20170306160146913, JCYJ20170306160932340).

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Authors and Affiliations

  1. Department of Clinical Laboratory, The People’s Hospital of Longhua, Shenzhen, 518109, China

    Xueyan Chen & Weichuan Zhong

  2. Food Inspection and Quarantine Center, Shenzhen Entry-Exit Inspection and Quarantine Bureau, Shenzhen, 518067, China

    Wenjun Chen

  3. School of Public Health (Shenzhen), Sun Yat-Sen University, Guangzhou, 510275, China

    Shaoyou Lu

  4. Shenzhen Center for Disease Control and Prevention, Shenzhen, 518055, China

    Shaoyou Lu, Zhi Tang, Zhou Zhu, Li Kang & Shicheng Liao

Authors
  1. Xueyan Chen
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  2. Wenjun Chen
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  3. Shaoyou Lu
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  4. Zhi Tang
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  5. Zhou Zhu
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  6. Weichuan Zhong
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  7. Li Kang
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  8. Shicheng Liao
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Correspondence to Shaoyou Lu.

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The authors declare that they have no conflict of interest.

Ethical Approval

The purpose of this study and the uses of urine samples were fully explained, and informed consent was obtained from the parents of the school children.

Human and Animal Rights

The study involving human participants have been approved by the ethics committee of Shenzhen Center for Disease Control and Prevention.

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Chen, X., Chen, W., Lu, S. et al. Development and Validation of HPLC–MS/MS Method for the Simultaneous Determination of 8-Hydroxy-2′-deoxyguanosine and Twelve Cosmetic Phenols in Human Urine. Chromatographia 82, 1415–1421 (2019). https://doi.org/10.1007/s10337-019-03757-2

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  • DOI: https://doi.org/10.1007/s10337-019-03757-2

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