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Preparation of Synthetic Amanitin Epitope Imprinted Polymers via Thiol-ene Click Reaction for Recognition and Extraction α- and β-Amanitins from Mushrooms

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Abstract

Extraction and purification of α- and β-amanitins from mushroom samples are still challenges. Surface-imprinted core–shell microspheres were synthesized combining target recognition determinant imprinting and thiol-ene click reaction, and applied to the extraction of α- and β-amanitins in mushroom samples. The highly efficient thiol-ene click reaction method offers a simple condition during the crosslinking process of molecularly imprinted polymers (MIP), and the functional monomer using could be successfully introduced onto the silica microspheres. The synthesized SiO2@MIP were characterized by scanning electron microscopy (SEM), Fourier transform infrared (FTIR), and thermogravimetric analysis (TGA). The measurement of SiO2@MIP resultants using high-performance liquid chromatography (HPLC) clearly demonstrates that SiO2@MIP not only display excellent affinity, but also show good selective recognition to α- and β-amanitins. The imprinted microspheres were successfully applied as sorbents for the extraction of α- and β-amanitins from mushrooms, attaining recoveries of 76.8–79.2%. Furthermore, the samples were effectively purified according to the chromatogram.

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Acknowledgements

This work was financially supported by a grant from the Natural Science Foundation of China (21275057), the Scientific and Technological Innovation Foundation of Foshan Gaoming (2014F08), and Project of Education Science Planning in Guangdong Province (2017GXJK186).

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Authors and Affiliations

  1. School of Environmental and Chemical Engineering, Foshan University, Foshan, 528000, China

    Song Xu, Jiean Tan, Xiaojuan Chen & Xin Chen

  2. School of Chemistry and Environment, South China Normal University, Guangzhou, 510006, China

    Yong Liang

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  1. Song Xu
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  2. Jiean Tan
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  3. Xiaojuan Chen
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  4. Xin Chen
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  5. Yong Liang
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Correspondence to Song Xu or Yong Liang.

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Xu, S., Tan, J., Chen, X. et al. Preparation of Synthetic Amanitin Epitope Imprinted Polymers via Thiol-ene Click Reaction for Recognition and Extraction α- and β-Amanitins from Mushrooms. Chromatographia 82, 1355–1363 (2019). https://doi.org/10.1007/s10337-019-03751-8

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  • DOI: https://doi.org/10.1007/s10337-019-03751-8

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