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Determination of Hormone Antagonists in Waste-Water Samples by Micellar Electrokinetic Chromatography

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Abstract

The contamination of water represents a major problem in many parts of the world. Endocrine disrupting compounds belong to the water contaminants and affect, besides other things, hormonal equilibrium in the living organisms. Such compounds are of different chemical structures and there is a demand for their fast determination in water samples. In our work, we focused on the development of a simple and fast method for separation and determination of five model hormone antagonists, exemestane, toremifene, letrozole, anastrozole, and mifepristone, by micellar electrokinetic chromatography. Within the best conditions, 50 mM sodium borate pH 9.5, 50 mM SDS, 15% 1-propanol, 20 kV, the separation of all the compounds lasted 8 min. The method was successfully validated with LODs of 1.2–7.7 µg mL−1, LOQs of 4.0–25.6 µg mL−1 and intraday repeatability of peak areas of 0.8–1.1%. Moreover, it was applied to the analysis of spiked water samples from a local waste-water treatment plant using SPE as a pretreatment step.

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Acknowledgements

The financial support of the research by the projects NPU LO 1305 (Ministry of Education, Youth and Sports of the Czech Republic), IGA_PrF_2018_027 (Palacký University in Olomouc, Czech Republic), and InnovaBalt (REGPOT-CT-2013-316149) project for M. Rucins is gratefully acknowledged.

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Authors and Affiliations

  1. Department of Analytical Chemistry, Regional Centre of Advanced Technologies and Materials, Palacký University in Olomouc, 17. listopadu 12, 77146, Olomouc, Czech Republic

    Martins Rucins, Daniel Baron & Jan Petr

  2. Latvian Institute of Organic Synthesis, Aizkraukles str. 21, Riga, 1006, Latvia

    Martins Rucins & Aiva Plotniece

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  1. Martins Rucins
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  2. Daniel Baron
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  3. Aiva Plotniece
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  4. Jan Petr
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Correspondence to Jan Petr.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Published in the topical collection Advances in Chromatography and Electrophoresis & Chiranal 2018 with guest editor Jan Petr.

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Rucins, M., Baron, D., Plotniece, A. et al. Determination of Hormone Antagonists in Waste-Water Samples by Micellar Electrokinetic Chromatography. Chromatographia 81, 1607–1612 (2018). https://doi.org/10.1007/s10337-018-3631-0

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  • DOI: https://doi.org/10.1007/s10337-018-3631-0

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