LC–MS/MS Determination of Catecholamines in Urine Using FMOC-Cl Derivatization on Solid-Phase Extraction Cartridge
A method for the determination of catecholamine derivatives in human urine is proposed that includes the derivatization of target compounds on a solid-phase extraction cartridge and determination of the analytes by a UHPLC method with tandem mass-spectrometric detection. 9-Fluorenyl-methoxycarbonyl chloride was used as the derivatization agent. The limits of detection for the analytes were 2.5 ng mL−1 for 9-fluorenyl-methoxycarbonyl-adrenaline, 5 ng mL−1 for 9-fluorenyl-methoxycarbonyl-octopamine, and 25 ng mL−1 for 9-fluorenyl-methoxycarbonyl-dopamine. The proposed procedure was tested on real samples obtained from volunteers.
KeywordsCatecholamines Derivatization FMOC-Cl UHPLC–MS/MS Solid-phase extraction
The studies were conducted as part of the Project No. 4.2612.2017/PC by the Ministry of Education and Science of the Russian Federation and were supported by the Russian Foundation for Basic Research, project No. 16-43-230404 r_a, using scientific equipment of the Ecological Analytical Core Facility Center of the Kuban State University, unique identifier RFMEFI59317Х0008.
Compliance with Ethical Standards
Conflict of interest
The authors declare that they had no conflict of interest.
All procedures performed in studies involving human participants were in according with the ethical standards fo the institutional and with the 1964 Helsinki decloration and its later amendments.
Informed consent was obtained from all individual participants included in the study.
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