Mono-, di-, and tetramethylglycolurils were synthesized, isolated, and purified. For the first time, the cis- and the trans-isomers of N,N-dimethylglycoluril were isolated as individual substances by semi-preparative HPLC method. The structures of the synthesized compounds were confirmed by 1H NMR, 13C NMR, and HR–HPLC–MS. The EI mass spectra of individual substances were obtained by the GC–MS. Retention and resolution of N-methyl glycolurils were investigated in the reversed-phase HPLC mode for different stationary phases: C18, SB–Aq, and Luna 5u PFP(2). The retention of N-methyl glycolurils depended on the amount of CH3 groups and distance between the CH3 groups in the structure. The stationary phases provided different selectivity for glycoluril and its N-methyl derivatives due to different shape selectivity. Complete separation of the N-methyl derivatives of glycoluril was achieved in 4.5 min on the stationary phase with pentafluorophenyl propyl ligand in a gradient mode.
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This research was supported by “The Tomsk State University competitiveness improvement programme” under Grant No. 220.127.116.118.
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All authors declare no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors. No compliance with ethical standards was involved.
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Kurgachev, D.A., Kotelnikov, O.A., Novikov, D.V. et al. Isolation, Identification, and Chromatographic Separation of N-Methyl Derivatives of Glycoluril. Chromatographia 81, 1431–1437 (2018). https://doi.org/10.1007/s10337-018-3599-9