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Preparation and Chromatographic Features of Fibrous Core–Shell HPLC Packing Material

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Abstract

In this study, fibrous core–shell silica particles were successfully synthesized via a one-step oil–water biphase stratification coating strategy. The core–shell silica particles were composed of 3-µm non-pore silica cores and thin shells (50–100 nm), which have radial-like direct channels and a large pore size (19.89 nm). The fibrous core–shell silica particles were further modified by n-octadecyltrichlorosilane and used as stationary-phase media in high-performance liquid chromatography (HPLC). The chromatographic properties of the particles were systematically studied in small-molecule and protein separation processes. The results showed that the back pressure was as low as 8.5 MPa under the 1.0-mL min−1 flow velocity. Furthermore, fibrous core–shell silica particles with an 80-nm shell were used for separating seven small molecules within 10 min and six proteins within 6 min. This work demonstrates that the fibrous core–shell silica particles could be used as an HPLC stationary phase with good performance and low back pressure, and that they have great potential for application to HPLC separation in the future.

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Acknowledgements

We thank Prof. Bai Quan’s group at Northwest University (Xi’an, China) for the non-porous SiO2 microspheres. This work was supported financially by Natural Science Foundation of Shaanxi Province (CN). (Grant No. 2018JM2039).

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Authors and Affiliations

  1. Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi’an, 710062, People’s Republic of China

    Wei Zhang, Shu-ming Li & Jing Zhang

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  1. Wei Zhang
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  2. Shu-ming Li
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  3. Jing Zhang
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Correspondence to Jing Zhang.

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Zhang, W., Li, Sm. & Zhang, J. Preparation and Chromatographic Features of Fibrous Core–Shell HPLC Packing Material. Chromatographia 81, 1249–1256 (2018). https://doi.org/10.1007/s10337-018-3571-8

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  • DOI: https://doi.org/10.1007/s10337-018-3571-8

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