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HTLC: A Viable Approach to Fast LC of Biogenic Amines in Food with Legacy Equipment

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Abstract

Nowadays, there is a demand for fast liquid chromatography (LC) of biogenic amines in food with the equipment available in the average laboratory. To this end, high-temperature liquid chromatography (HTLC) is presented in this study as a viable option, working on a conventional LC platform at moderately high temperatures up to 80 °C. The LC platform was adapted by including an appropriate length of stainless-steel microbore tubing as a preheater. Biogenic amines as benzoyl derivatives showed good thermostability on a thermally rugged column (150 × 4.6 mm, 5 µm). As a model application the HTLC conditions were developed for wine separation. The separation selectivity changed considerably with temperature in the range 36–84 °C, and baseline resolution was obtained only at temperatures well above ambient. At 73 °C oven temperature, the system allowed a flow rate as high as 3 mL min−1 with the backpressure not exceeding 195 bar (2800 psi). The separation took less than 5 min, comparably fast to documented fast LC separations, and typically at least three to five times faster than a conventional separation.

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Acknowledgements

The authors are grateful to the financial support from the National Key Research and Development Program of China (Grant number 2016YFD0400301).

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Authors and Affiliations

  1. Department of Food Science and Nutrition, Fuli Institute of Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang R&D Center for Food Technology and Equipment, Zhejiang University, Hangzhou, 310058, China

    Jian-Jun Zhong, Tian Ding, Xingqian Ye & Donghong Liu

  2. Department of Nutrition and Food Safety, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, 310006, China

    Ningbo Liao

  3. College of Life Sciences, Tarim University, Alar, 843300, China

    Yunfeng Pu

Authors
  1. Jian-Jun Zhong
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  2. Ningbo Liao
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  3. Yunfeng Pu
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  4. Tian Ding
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  5. Xingqian Ye
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  6. Donghong Liu
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Correspondence to Donghong Liu.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Zhong, JJ., Liao, N., Pu, Y. et al. HTLC: A Viable Approach to Fast LC of Biogenic Amines in Food with Legacy Equipment. Chromatographia 81, 1097–1102 (2018). https://doi.org/10.1007/s10337-018-3539-8

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  • DOI: https://doi.org/10.1007/s10337-018-3539-8

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