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Isolation and Purification of Three Analogues from Clematis akebioides by Molecularly Imprinted Solid-Phase Extraction and HSCCC

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Abstract

An efficient strategy of using molecularly imprinted solid-phase extraction and high-speed counter-current chromatography (HSCCC) were established for separation and purification of three analogues from Clematis akebioides for the first time. The three analogues, including 26.30 mg caffeic acid, 84.20 mg p-coumaric acid, and 44.40 mg ferulic acid, were obtained from 200.00 mg of the crude sample. The molecularly imprinted polymer was prepared with caffeic acid as template molecule and acrylamide as functional monomers. The results showed that the caffeic acid and its analogues could be well enriched. Then, the extract was purified by HSCCC using a two-phase solvent system consisting of chloroform/acetonitrile/water/acetic acid (10:5:4:0.0004, v/v) and a continuous sample introduction system was adopted. The three organic acids were identified by 1H-NMR and 13C-NMR.

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Acknowledgements

This work was supported by the priority academic program development of Jiangsu higher Education Institutions (1107047002), Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources (Guangxi Normal University) (CMEMR2016-B06 to ZXL), and Instrument Function Development Project of CAS (no. 2017g107).

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Authors and Affiliations

  1. School of Chemistry and Chemical Engineering, Southeast University, Nanjing, 211189, People’s Republic of China

    Hongliu Liu, Chengcheng Gong & Zhixin Liao

  2. Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, 810001, People’s Republic of China

    Tao Chen & Yulin Li

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  1. Hongliu Liu
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  2. Chengcheng Gong
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  3. Zhixin Liao
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  4. Tao Chen
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  5. Yulin Li
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Correspondence to Zhixin Liao or Yulin Li.

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Liu, H., Gong, C., Liao, Z. et al. Isolation and Purification of Three Analogues from Clematis akebioides by Molecularly Imprinted Solid-Phase Extraction and HSCCC. Chromatographia 80, 1651–1658 (2017). https://doi.org/10.1007/s10337-017-3406-z

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  • DOI: https://doi.org/10.1007/s10337-017-3406-z

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