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Simultaneous Cell Disruption Extraction and Purification Followed by Two-phase Derivatization Procedure for the Determination of Organotin Compounds in Wet Biological Samples by GC–MS

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Abstract

Freeze-dried biological samples used for the determination of organotin compounds (OTCs) involve complicated or tedious pretreatment (e.g., freeze-drying) that may result in loss of analyst or contamination. In the present study, a rapid and accurate method for determination of five kinds of OTCs—monobutyltin (MBT), dibutyltin (DBT), tributyltin (TBT), diphenyltin (DPhT) and triphenyltin (TPhT) in fresh biota samples—was developed and further compared with freeze-dried tissues. It involved a sample preparation method of simultaneous extraction and purification of OTCs in fresh biological samples, which was developed with ultrasonic cell disruption and primary secondary amine (PSA) adsorption, and followed by two-phase (organic solvent and cell membrane phases) derivatization before the analysis by gas chromatography–mass spectrometry. The extraction efficiency from biota matrices was relatively better with the acetate/acetic acid and tropolone into methanol as extraction agents and primary secondary amine as grease-removing agents. The retention of cell membrane-like materials as well as 25% of ethyl acetate as co-extraction reagents used after centrifugation improved the targeted OTCs’ recoveries. The proposed method, under the optimal condition, has shown satisfactory performance in wet biological samples. The limits of detection obtained were less than 10 ng Sn g−1 ww (wet weight) for all analytes. Spiked recoveries were within the range 89–118% and the relative standard deviations were less than 7% (n = 5) when quantified by matrix-matched calibration curves. Finally, the applicability of this new method was further verified by comparing the results from wet and freeze-dried oyster tissues collected from two coastal cities in China.

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Acknowledgements

The authors thank the support from the State Key Laboratory of Pollution Control and Resource Reuse (no. RCRRE 16011) and Key Laboratory of Yangtze River Water Environment of the Ministry of Education (no. YRWEF201601).

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Authors and Affiliations

  1. State Key Laboratory of Pollution Control and Resources Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai, China

    Chunzhao Chen, Ling Chen, Lingling Wu & Qinghui Huang

  2. State Key Laboratory of Marine Environmental Science, College of the Environment and Ecology, Xiamen University, Xiamen, Fujian, China

    Zhaoying Chen

  3. John A. Reif, Jr. Department of Civil and Environmental Engineering, New Jersey Institute of Technology, Newark, NJ, USA

    Wen Zhang

Authors
  1. Chunzhao Chen
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  2. Ling Chen
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  3. Zhaoying Chen
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  4. Wen Zhang
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  5. Lingling Wu
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  6. Qinghui Huang
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Correspondence to Qinghui Huang.

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Funding

This study was funded by the State Key Laboratory of Pollution Control and Resource Reuse (no. RCRRE 16011) and Key Laboratory of Yangtze River Water Environment of the Ministry of Education (no. YRWEF201601).

Conflict of interest

Author Chunzhao Chen declares that she has no conflict of interest. Author Ling Chen declares that she has no conflict of interest. Author Zhaoying Chen declares that she has no conflict of interest. Author Wen Zhang declares that he has no conflict of interest. Author Lingling Wu declares that she has no conflict of interest. Author Qinghui Huang declares that he has no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Chen, C., Chen, L., Chen, Z. et al. Simultaneous Cell Disruption Extraction and Purification Followed by Two-phase Derivatization Procedure for the Determination of Organotin Compounds in Wet Biological Samples by GC–MS. Chromatographia 80, 1659–1667 (2017). https://doi.org/10.1007/s10337-017-3399-7

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  • DOI: https://doi.org/10.1007/s10337-017-3399-7

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